LOCUS       sequence-304771-        5745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the AVAST type 5 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG021 (AVAST type 5)
FEATURES             Location/Qualifiers
     source          1..5745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3525..3572
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3792..4451)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4366..4385
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4452..4554)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4946..4963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5080..5625)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5160..5179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaactag ctaagcaata
      121 agggcgatcg gctctcccat agatcgaggc cgaatgatgt tagcaatgtt cactcttggc
      181 tggaatctgc cagaaatcga ggtcatatgg tctgctttga gtgaggagcg caaatggata
      241 aagccctcat gagttctttt tcaatgacct aacttttgag aggcactggg ttagatcatg
      301 tttcatgttt gcaatacaat atatatttaa acttaggttt ataacttaaa tgttagttcc
      361 tgatctaaac cagattatta atcactccta gagtgaaatg agttaagcca agagttgata
      421 aaattaacag ttttttttac aatatctgga tgtttgctag cgaacaggca tctaaaataa
      481 ctatgctgag ctaaacttac aattcaaatt gtaccgagga taaaatgcaa gtacaacatc
      541 atactgaacc aaacttgaag aatgagattg tggctttatt taaggcttct caattgatac
      601 ctttttttgg cagtggattt actagagata ttagagcaaa aaatggtaaa gttcctgatg
      661 ctattaaatt tacggagttg attaggaata tagcggcaga aaaagaaggg ttaacacaaa
      721 cagaaataga tgaaattcta agaatcagcc agcttaaaaa agcgtttgga cttctaaata
      781 tggaggaata tatacccaaa cgaaaatcga aggcattatt aggtaacatt ttttcagagt
      841 gtaaactctc tgatcacgaa aagacaaaaa taataaattt agattggcct catattttca
      901 cgtttaatat tgacgatgct atagaaaacg ttaataggaa atacaaaatt ctgcatccaa
      961 atcgagcagt tcagagagaa tttatatctg ctaataagtg tctattcaaa attcatggcg
     1021 atattactga atttattaaa tacgaagatc aaaatctgat atttacttgg cgtgaatatg
     1081 cacacagtat agaagaaaat aaatccatgc tatccttttt atctgaggaa gccaaaaact
     1141 cagctttcct tttcataggt tgcagtcttg atggagagct tgatttaatg catttatcaa
     1201 gaagcacacc atttaagaaa tcaatttatt tgaagaaagg atatttaaat ttagaagaaa
     1261 aaatagctct ttcggagtac ggcatcgaaa aagtaattac ctttgacact tacgatcaga
     1321 tatatcaatg gttaaataac acacttcaga atgttgagcg aaaatccccc acaagaagtt
     1381 tcgaactcga tgactccaag ttaatgaaag aagaggctat aaatttattc gctaatggag
     1441 gccctgtaac taaaatagtg gataataaaa gaatcctgcg aaattctata actttttctc
     1501 aacgagatgt ctgtgatgat gcaattaaag cactacgtaa tcatgactat atcctaatta
     1561 caggtcgacg tttcagcgga aaatctgtac ttttatttca aattattgag gcaaaaaaag
     1621 aatataatgc ctcttattac tcttcgactg acacattcga tccttccatt aaaaactcat
     1681 tgataaaatt cgagaatcat atattcgttt tcgactctaa tttctttaat gcacaaagca
     1741 ttgatgaaat tttaaccaca agggtgcatc ctagtaacaa agttgtttta tgctcgagtt
     1801 ttggtgacgc agagttatat agattcaagt taaaggataa aaagatatta cataccgaaa
     1861 ttcagattaa aaataacttg attaatgaag aaggtaacta tctcaatgat aagctttctt
     1921 ttgaggggct accactttat aaatcttcag aaacgttgtt gaattttgct tatcgatact
     1981 atagcgagta taaaaataga ctaagtggtt ctaatttatt taataagcaa tttgatgaag
     2041 attcaatgtt tgttttgatt ttaattgcag cttttaataa agccacatat ggtcatatca
     2101 acagtcacaa taaatatttt gatattcaga attttatttc gcaaaatgat agattatttg
     2161 aattggagtc aactaacaca gatccaagtg gagttataat ctgcaattca ccatcctggc
     2221 ttttaagagt tatcagtgag tatattgata agaatcctgc atcttataaa acagtatctg
     2281 atttaataat atctcttgcg tcaaaaggat ttcttgcagc atcaaggaac cttataagct
     2341 ttgataaact aaatgaactt gggaatggaa aaaatgtcca taaatttatc aggggtatat
     2401 ataaggaaat tgcacatacc tatcgtgaag atatgcacta ctggttacaa agggctaagt
     2461 cagaattaat atcggcacac acaattgatg acctcgtcga aggaatgagt tatgcaagca
     2521 aagtaagact cgatagtgcc gagtttaaaa atcaaactta ttacagtgcc acattagtat
     2581 tagcgcagtt gtctgcaagg gctctatcta taaataatga taaaatatat gcgctgagct
     2641 tctttgaaag tagcctagaa tccatccgga attataataa taactcaagg cacataaaca
     2701 aaatgatgga taaaaatgat ggtggcttta gatatgcaat acaatatctt aaggataatc
     2761 cattaataga actccttcct cgtaaggacg aagttaatga attaattaac ttctatgaga
     2821 gtcgtaagaa ataatcatcc ttaaattaat aaatggcaag taactcattc ccttgtcatt
     2881 tattaaactc ttaagagcct tatcccgaaa agtattaatc tgagctaata agattgtttt
     2941 tcagctatgt cattatttta ttgccaatat atttacactt aagcattgac aggtagcgga
     3001 tagttatttt tggcttgtaa ataagccttt taataataga actgtaagac aatcgctctg
     3061 attttttgaa atttatctca atgttaaatt cttccgcttt tggcacaaac gggctagagc
     3121 agacagattt aatgagataa gggtatagat gaattctcca tacccttgaa cgattacttc
     3181 ccagttgatt tgcttggttt cagtcctggg gtattaccgg gtgtatcctt attatcacgt
     3241 ctgcgttgat cgggttttcc tgttgatttt gcaattggtt ttggaccagg tttaagcccc
     3301 ataatcgtac tccttagcca tgtcagaggt tattcctcag tgtggatata aggggagcgg
     3361 taagaattat caagcttgga tgggcggtga aaaatgacta cttgactatt atgtgagcaa
     3421 tgtcagcttt tgacatttag aggccagccc attactgaag taagccaaaa atgagtcgcg
     3481 atgagccctc aacaatgagg gccacctcgg agattgcgca ataactagca taaccccttg
     3541 gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcattt gagaagcaca
     3601 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
     3661 tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt
     3721 catccgctta ttatcactta ttcaggcgta gcaccaggcg tttaagggca ccaataactg
     3781 ccttaaaaaa attacgcccc gccctgccac tcatcgcaat actgttgtaa ttcatttaac
     3841 attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc
     3901 agcaccttgt cgccttgcgt ataatatttg cccatagtga aaacgggggc gaagaagttg
     3961 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     4021 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     4081 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     4141 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     4201 atcaccagct caccgtcttt catcgccata cggaactctg gatgagcatt catcaggcgg
     4261 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     4321 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     4381 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     4441 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     4501 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct
     4561 cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt
     4621 tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat
     4681 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt
     4741 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
     4801 cgccggacat cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt
     4861 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata
     4921 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     4981 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
     5041 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
     5101 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     5161 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
     5221 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
     5281 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     5341 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag
     5401 caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc
     5461 ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct
     5521 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
     5581 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
     5641 aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
     5701 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgc
//
