LOCUS       sequence-304772-        6546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the dsr1 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG022 (dsr1)
FEATURES             Location/Qualifiers
     source          1..6546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4326..4373
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4593..5252)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5167..5186
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5253..5355)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5747..5764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5881..6426)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5961..5980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcatattt tgcgtagcta
      121 gaacgcaatc aaatctagca gtccgctttg ttcggagttc ggacattatg agttggcaag
      181 taaagtagct tgctaggaag ccggatttgc acggtcggta taataagatg taaccccttg
      241 ccttcattta ctcgaatgaa cgtgcacatt ggataggagg aaaaggaatg caattcatta
      301 ccaacggccc tgatattcct gatgagcttt tgcaggcgca cgaggaaggg cgcgttgtgt
      361 tcttctgtgg agcaggcatt tcctaccctg ctggtttacc tggtttcaaa gggttggtag
      421 aactaattta ccagaggaac ggaacaacac tttcagaaat tgagcgtgag gttttcgagc
      481 gtgggcaatt tgacggcaca ttagatttgc tggaacggcg cttaccaggg cagcgtatag
      541 ccgtccgacg cgcgttggaa aaagccctta agccaaagct ccgtcgtagg ggcgctattg
      601 atactcaggc ggcgctgtta cgtttagccc gtagccgcga gggtgccctt cgattggtca
      661 ctaccaactt tgaccgtctc tttcatgtgg cagctaaacg tacaggccag gcttttcagg
      721 cctatgtagc gccgatgctg ccaattccaa aaaacagccg ctgggatgga cttgtatacc
      781 tgcatgggct gttaccggaa aaggcggatg atactgccct gaatcgtctg gttgttacca
      841 gcggtgactt tggcttggct tatctcactg agcgttgggc agctcgcttt gtgagtgagt
      901 tatttcgtaa ctatgtggtc tgcttcgttg gctacagcat caacgacccg gtactgcgct
      961 acatgatgga tgcgcttgca gcagatcgga ggctcggtga agtcacacca caagtatggg
     1021 cactggggga gtgtgagccg gggcaggagc accggaaagc catcgagtgg gaggccaaag
     1081 gggtcactcc tatcctttac accgtaccgg cgggctccac tgatcattca gtgctgcatc
     1141 aaacgttgca cgcttgggca gatacttatc gagatggtat acagggcaaa aaggctatag
     1201 tcgtcaaaca tgctctggcc cgcccgcagg acagcactcg tcaggacgat ttcgttggtc
     1261 ggatgttgtg ggccttgtca gataaatcag gtttaccagc aaaacgcttt gcggaactca
     1321 atcctgcacc gccgctggat tggttattga aagctttctc ggacgaacga tttaaataca
     1381 gcgatctgcc acgcttttgt gtatctccgc atgtcgaaat tgacccgaaa ctccgattca
     1441 gtctggttca gcgtcctgcg ccctatgagc tggccccgca gatgtcgctg gtttctggat
     1501 gtgtcagtgc tagcaaatgg gatgacgtaa tgtcccatat agcccgttgg ctagttcgtt
     1561 atctgggcga ccctaggttg atcatatgga ttgctgaacg cggcggacaa atacacgacc
     1621 gttggatgtt tctgattgag agcgaactag atcgcttagc agcactgatg cgggagcgta
     1681 agacttctga gttagatgaa attctcttgc attcccccct ggctattcct ggtccaccta
     1741 tgtctacttt atggcggctt ctgcttagtg gtcgtgtgaa atcgccattg cagaacctgg
     1801 atttgtatcg ttggcaaaac cgcttaaaga atgaaggctt gacgactaca ttgcgcttgg
     1861 agttacgcgg gttgctttct cccaaggtta tgttgaggcg gccgtttcgc tatagtgaag
     1921 acgattcgag cagcactgat gaacccttgc gaatcaagca attggtggat tgggagctgg
     1981 tgctgactgc tgattacgta cgttcaaccc tgttcgacct tgctgacgag tcatggaaat
     2041 cgtccttgcc atacctgttg gaagattttc agcagttgtt gcgtgatgca ctggacttgt
     2101 tgcgggagtt gggagagtcc gacgatcgtc acgaccgctc gcattgggat ttgccgtcca
     2161 tcactccgca ctggcagaac cgggggttcc gcgattgggt gagcctgatt gaattacttc
     2221 gggattcatg gttagccgtt cgagccaaag acagcgatca ggcctcgcgc attgctcaga
     2281 attggtttga gttgccatat cccaccttca aacgtctggc actgtttgcc gcaagccaag
     2341 acaactgcat accacctgag cggtgggtta attggttgtt agaggacggt tcatggtggt
     2401 tgtgggccac ggatactcgg cgagaggtat tcagactgtt tgttttgcag ggacgacatc
     2461 tgacaggaat tgcacaagag cgtctggaaa ctgctatctt ggcagggcct ccgcgcgaga
     2521 tgtacgagga taatttggaa gcagacaggt ggcattattt ggtggctcat tccgtctggt
     2581 tgtgtctagc gaagctcagg ggagcgggcc ttgttttggg agagtctgcg gctacacgtt
     2641 tgacggaaat atccacagca tacccaaaat ggcaactggc aaccaacgag cgtgatgaat
     2701 tctctcactg gatgagcgga accggtgatc caggcttcga ggagagtata gatgtcgaca
     2761 ttgcgccccg taagtggcag gaattagtgc aatggctcgc aaagcctatg ccagaaagac
     2821 tgcctttcta tgaggacact tggagtgatg tttgccgtac gcgctttttt cacagtctgt
     2881 atgcgttacg taaactatca caagatgatg tgtggcctgt tggtcggtgg cgtgaagctc
     2941 tgcagacttg ggctgaacca gggatgattt tgcgttcgtg gcggtacgcc gcaccgttgg
     3001 tgcttgacat gcctgacgca gtacttcagg agatttccca cgctgtcact tggtggatgg
     3061 aggaggcttc gaagaccatc ctctgccacg aggagattct actggccctt tgtcgtcggg
     3121 ttctgatgat agaaacaagc ccagagtcta gcaccattcg aaacggaatt gagacctatg
     3181 atcctgtttc tacggcgatc aatcatccca ttgggcatgt cacgcaatca ctgatcaccc
     3241 tatggttcaa acagaacccg aatgacaatg atttgcttcc tgttgaattg aaaacacttt
     3301 tcaccaaatt gtgtaatgta cagatagagc tattccgcca tggtcgggtg ttgctggggt
     3361 cgcggctgat cgcatttttt cgcgtagatc gaccttggac cgaacagtat ctattgccct
     3421 tgtttgcttg gagtaatccc gtcgaagcaa aagctgtgtg ggaaggcttc ctctggtcgc
     3481 cacgcctgta tgaaccgttg ctgatagctt tcaagtcaga ttttttggag agcgccaatc
     3541 actattctga tcttggcgag caccggcagc aattcgctat tttcctgact tatgcagctc
     3601 tgggccctac cgagggatat accgtggagg agttccgaac ggcaattagt gctcttccac
     3661 aagaaggtct ggaggtagcc gcgcaggcgt tataccaggc acttgaaggt gcgggcgatc
     3721 agcgcgagga gtattggaaa aatcgtgtcc agccattttg gcaacaggtt tggccaaagt
     3781 cccgcaactt ggccacccca cgcatatccg aatcgttgac tcgtatggtg attgctgccc
     3841 gaggtgaatt tccggcggct ttggcagtgg tgcaggactg gctgcaaccg ctcgaacacc
     3901 ttagctacga cgttcgcctt ttgctagaat cagatatttg cagccgatat cctgcggacg
     3961 ctctatccct gctgaatgcc gtgattgccg aacaacactg ggggcctcga gagttggggc
     4021 aatgcttgct tcaaattgtt caagctgctc cacaactgga gcaagatgtt cgttatcagc
     4081 gattaaatga atattctcga aggcgcagcg tgtgaaagtg acaggcgttg gacagtgcga
     4141 actgtggagc ctaacaaggt aaagacactc taactgataa tgctgcgccg ctcgtgcaat
     4201 gcaatacagt ttttatctag cggtgaatta tggtgttaaa agttagcccc tgacacaggg
     4261 tgggtagttg gctctgtgtc attgatgggt attagttctg atatgagcta atacccacgc
     4321 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaac
     4381 ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc
     4441 agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt
     4501 tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt agcaccaggc
     4561 gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcaa
     4621 tactgttgta attcatttaa cattctgccg acatggaagc catcacagac ggcatgatga
     4681 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatagtg
     4741 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     4801 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     4861 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     4921 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     4981 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcatcgccat acggaactct
     5041 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     5101 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     5161 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     5221 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     5281 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     5341 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
     5401 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
     5461 gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
     5521 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt
     5581 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac
     5641 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg
     5701 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact
     5761 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
     5821 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
     5881 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     5941 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
     6001 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
     6061 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     6121 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6181 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
     6241 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
     6301 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
     6361 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
     6421 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
     6481 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgttag
     6541 tcatgc
//
