LOCUS       sequence-304774-        5642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the SIR2 + HerA defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG024 (SIR2 + HerA)
FEATURES             Location/Qualifiers
     source          1..5642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3422..3469
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3689..4348)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4263..4282
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4349..4451)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4843..4860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4977..5522)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5057..5076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcatagct attgtgacta
      121 tgctaaccat atgaatctat tgtgtgatta tgagtaatga ctttttctaa tatttgattt
      181 ttaatgtagt aacttagcta attttaaaat ttgtaaaagg atgtttatgt cgatttatca
      241 aggtggtaac aagttaaatg aggatgattt tcgttctcac gtttattcct tgtgtcaatt
      301 agataatgtt ggcgttctgt taggtgctgg tgcttctgtc ggttgtggtg ggaaaacgat
      361 gaaagatgta tggaaatcgt ttaagcaaaa ctaccctgag cttttgggag cacttattga
      421 taaatatctt ctggtttcgc aaattgattc tgataacaat ttggtcaatg ttgaactttt
      481 gatagatgaa gcaactaaat ttctttctgt agctaaaact agacgatgtg aagatgaaga
      541 ggaggaattc aggaaaatat taagttcatt atataaagag gttacgaagg ctgcattatt
      601 aacaggagaa cagtttagag agaaaaatca gggtaaaaaa gatgcgttta aatatcacaa
      661 agagttaatt tcaaaattaa tttcaaatag acagcccggt cagtcggctc cggcaatttt
      721 tacaacaaat tatgatttgg ccttagagtg ggctgcagaa gatttaggaa tacagttgtt
      781 taatggtttt tctgggctac atacacggca gttttatccc cagaattttg atttggcttt
      841 cagaaatgta aatgcgaagg gcgaagcaag attcggacat tatcatgcgt atctctataa
      901 attacatggc tcacttacgt ggtatcaaaa tgatagcttg actgttaacg aagttagtgc
      961 atctcaagca tatgatgaat atattaatga cataatcaat aaagatgact tttatcgcgg
     1021 tcaacatttg atttatccag gggcgaataa atatagccat acaatcggct tcgtttatgg
     1081 agagatgttt agacgttttg gggagtttat ttcgaaacct caaacagcgt tgttcataaa
     1141 tgggtttggt ttcggtgatt atcatataaa tagaataata ttaggcgcgt tactgaatcc
     1201 atctttccat gttgttatat attatcctga attgaaagaa gcaattacca aagtaagtaa
     1261 gggtggtggt tcggaagctg agaaagctat tgttacttta aaaaatatgg ctttcaatca
     1321 agtaactgta gttgggggag gaagcaaggc atattttaat agtttcgtag aacatctacc
     1381 ataccctgtg ctctttccac gagataatat tgttgatgag ttggttgaag caattgctaa
     1441 tctttctaaa ggagaaggta atgtcccttt ttaaacttac tgaaatctcg gctattggat
     1501 acgttgtagg attagaaggg gaaagaatta ggataaacct gcatgagggg ttgcaaggca
     1561 gattagcatc gcatagaaag ggggtgagct cagtaacgca accaggagat cttattgggt
     1621 tcgatgcagg taatatatta gttgtcgcaa gagtgacaga tatggcattt gttgaagcgg
     1681 ataaagcgca taaggcaaat gtaggcacat ctgatttagc tgatatacct ctaagacaaa
     1741 ttatcgccta tgcaattggc tttgtgaaaa gggagttaaa tggttatgtt tttatatcag
     1801 aagattggcg cttacctgca ttgggttctt ctgctgttcc tttgacttca gattttttga
     1861 acatcattta tagtattgat aaagaagaac tcccaaaagc ggttgaatta ggtgtggatt
     1921 ctagaactaa aaccgttaag atatttgcaa gtgttgataa attattgtcg cgacacttag
     1981 ccgttcttgg tagtacagga tatggtaaat caaatttcaa tgctttgtta acgaggaagg
     2041 tttctgaaaa ataccctaac tcaagaatag ttatttttga cataaatggt gaatacgcgc
     2101 aagcttttac aggtattcca aatgtaaagc acactattct aggggaatcc ccaaatgttg
     2161 atagtttgga aaaaaagcag caaaagggtg agctatatag tgaagagtat tattgttata
     2221 aaaagatacc atatcaggca ttaggttttg ctgggttaat taaattatta agaccaagtg
     2281 ataaaacaca attgcccgca ttaagaaatg cattaagtgc aattaatcgg actcatttta
     2341 aaagccgtaa tatttacttg gaaaaagatg atggtgaaac ttttcttttg tatgatgatt
     2401 gtcgtgacac aaatcaaagt aaattggctg agtggttgga tttattaagg cgtagacgtc
     2461 ttaaaagaac gaatgtatgg ccaccgttta aaagtttagc gactttggtt gctgaatttg
     2521 gatgtgtagc tgctgaccgt tctaatggaa gtaaacgtga cgcgtttggt tttagtaacg
     2581 tgttgccatt ggtaaaaatc atacaacaac ttgcagagga tataagattt aaatctattg
     2641 ttaatttaaa tggagggggt gagctagcag atggtggaac gcattgggat aaagctatga
     2701 gtgatgaagt tgattacttc tttggtaagg aaaaaggaca agaaaatgat tggaatgttc
     2761 atatagttaa tatgaaaaat ttggcacaag atcatgctcc aatgttactt agtgcattgt
     2821 tggagatgtt tgctgagata ctatttagac gtgggcagga acgttcgtat cctacggtac
     2881 ttttgttgga agaagcgcat cattacctgc gtgaccctta tgctgaaatt gactcacaga
     2941 ttaaagcata tgaacgactt gctaaagaag gtaggaaatt caaatgctct ttaattgtca
     3001 gtactcagcg accctcagag ctttctccta ctgttttggc aatgtgttca aactggtttt
     3061 cgttacgttt gactaatgaa agagatttac aggctctcag atatgcaatg gaaagcggta
     3121 atgaacaaat cttaaaacaa atatcaggtt taccaagagg tgatgctgtt gcatttggtt
     3181 ctgcatttaa tttgcctgta agaatttcaa ttaatcaagc aaggccaggg ccaaaatctt
     3241 cagatgctgt tttttctgaa gaatgggcta attgtacaga attacgttgt taattacctg
     3301 atgtacatgg ctagtgcaag ttggtagcgc atgtctatat gcatttattt gcatgtgttt
     3361 tattgagtga gcgcacaagc ttgatgaccc gacaggtatg tatttagact gaacgcaata
     3421 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
     3481 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     3541 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     3601 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca ccaggcgttt
     3661 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcaatact
     3721 gttgtaattc atttaacatt ctgccgacat ggaagccatc acagacggca tgatgaacct
     3781 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atagtgaaaa
     3841 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     3901 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     3961 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     4021 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     4081 ggtgaacact atcccatatc accagctcac cgtctttcat cgccatacgg aactctggat
     4141 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     4201 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     4261 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     4321 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     4381 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     4441 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     4501 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     4561 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     4621 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
     4681 cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg
     4741 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
     4801 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     4861 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     4921 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     4981 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     5041 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     5101 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     5161 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     5221 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5281 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     5341 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     5401 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     5461 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     5521 aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt
     5581 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat
     5641 gc
//
