LOCUS       sequence-304775-        5753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the DUF4297 + HerA defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG025 (DUF4297 + HerA)
FEATURES             Location/Qualifiers
     source          1..5753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3533..3580
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3800..4459)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4374..4393
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4460..4562)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4954..4971)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5088..5633)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5168..5187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagtgcg ccttatgtga
      121 ttacaacgaa aataaaaacc atcacacccc atttaatatc agggaaccgg acataacccc
      181 atgagtgcaa tagaaaattt cgacgcccat acgcccatga tgcagcagta ttgaaaaata
      241 taacatatcc aactgattgt attgaaaatt taaaatagcc atataacaaa aggttacaca
      301 taagctactt tttggggttt caggcaagaa actaaaaatt attaacgcca tcaaattatt
      361 cacatcttaa taattagcat tgaaatttaa tgtttttggt tctttgtaca tgtcaatggc
      421 ttgtctttgt ggcagaatca taaagctatg caatcattgc attgttatta acacagcata
      481 tttttatata cttttaacac cttacctcaa aaaggataac aaagtggaca gaagtgcggt
      541 tgatacaatt cgtgggtatt gttatcaggt tgataaaacg attattgaga ttttttcgtt
      601 accacaaatg gatgactcga ttgatataga gtgcattgaa gatgttgatg tctacaacga
      661 tgggcattta actgcgatac aatgcaaata ttatgaaagt accgattata accactccgt
      721 tatatcaaag cccataagat taatgttgtc acactttaag gacaataaag aaaaaggggc
      781 taattattat ctttatgggc attataaatc cggtcaagaa aagttaacac tcccattaaa
      841 agttgacttt ttcaaatcta atttcctcac ctacaccgaa aaaaaaatca aacatgaata
      901 ccatattgaa aatgggctta ccgaagagga tctacaagcc tttttggatc ggttagttat
      961 aaatatcaat gcaaaatcat ttgatgatca aaaaaaagaa actatacaaa taataaaaaa
     1021 ccatttccaa tgtgaagatt atgaggcaga gcattatctt tattctaatg ctttcagaaa
     1081 aacatatgat atctcttgta ataaaaaaga tagaaggata aaaaaatctg attttgttga
     1141 aagtatcaac aaatcaaaag tcttatttaa catatggttt tatcaatatg aaggaagaaa
     1201 agaatattta agaaaattaa aagaatcttt catacgcaga agtgtaaaca cctcacctta
     1261 tgctcgtttt ttcatcttag aatttcaaga caaaactgat ataaaaacag ttaaagactg
     1321 tatatataaa atacaatcaa attggtctaa tttatctaaa agaacagatc gaccatattc
     1381 tcctttttta ctttttcatg gcaccagcga tgccaattta tacgaattaa agaatcaatt
     1441 attcaatgaa gatctaattt tcactgatgg gtaccctttt aaaggaagtg tatttacccc
     1501 caagatgtta atcgaaggtt tttcaaataa agaaatccac ttccaattta tcaacgacat
     1561 agatgatttc aatgaaacac tgaacagtat taatataaga aaagaagttt accagtttta
     1621 tacggaaaac tgccttgata tcccatccca actaccccag gtaaacatac aagttaaaga
     1681 ctttgccgac ataaaggaga tagtgtaatg agcaggaata atgatattaa tgcagaagta
     1741 gtatcggtat cgccaaataa attaaaaatt tccgtagacg atcttgaaga atttaagata
     1801 gcagaagaaa aattaggtgt aggatcttat ttaagggttt cagataatca agatgttgct
     1861 cttctggcga tcatagataa tttttctatt gaagttaaag aaagccaaaa gcagaaatac
     1921 atgatagaag caagtccaat aggtcttgtt aaaaatggaa aattctatcg cggtggagat
     1981 tcacttgcac ttcctcctaa aaaagtggaa ccagcgaaat tagacgaaat aatatccata
     2041 tactcagata gtatagatat aaatgaccgt tttacttttt caagcttatc gcttaatacc
     2101 aaagtatccg tacctgtgaa tgggaataga tttttcaata aacatatcgc tatcgtaggt
     2161 tcaacgggtt caggtaaatc ccacactgtt gcaaaaatac ttcaaaaagc cgtagatgaa
     2221 aagcaagaag gttataaggg attaaacaat tctcatataa ttatttttga tatacattct
     2281 gaatatgaaa atgcattccc taattcaaat gtattaaatg tagatacatt aacccttcca
     2341 tattggctat taaatggtga cgagttagaa gagctttttc ttgacacgga agcaaatgat
     2401 cacaatcaaa gaaatgtgtt ccgtcaggca ataacattaa ataaaaagat acattttcaa
     2461 ggagatccag ccacaaagga aataataagc tttcactcgc catattattt cgacattaat
     2521 gaagtcatca attatattaa caatagaaat aatgaaagaa aaaataaaga taatgaacat
     2581 atttggtcag atgaggaagg aaatttcaag tttgacaatg aaaatgctca taggttattc
     2641 aaagagaatg taactcctga tggaagttca gccggtgctt taaatggaaa acttctcaat
     2701 tttgttgatc gattacaaag taaaatattt gataagagat tagattttat tctgggtgaa
     2761 ggtagcaaat ccgtaacatt taaagaaaca ttagaaactt taataagcta tggaaaagat
     2821 aaatcaaaca taacaatact tgatgtaagc ggtgttcctt ttgaagtact tagcatatgt
     2881 gtatcattga tatctcgatt aatttttgaa tttggctatc attcaaaaaa aataaaaaga
     2941 aaatctaatg aaaaccaaga tatcccaata ttaattgttt acgaagaagc acataaatat
     3001 gctcccaaaa gtgatctgag caaatacagg acatccaaag aagcaattga gaggattgca
     3061 aaagagggta gaaaatacgg agtaaccctt ctccttgcaa gtcagagacc ttctgaaatt
     3121 tcagaaacaa tattttctca gtgtaatact tttatctcaa tgcgattaac taacccagac
     3181 gatcaaaatt atgttaagcg attactcccg gatacagtag gtgatattac aaacctccta
     3241 ccatcgctca aagaaggtga ggccttaatc atgggggatt caatatcaat accttcgatt
     3301 gtaaaaatag aaaaatgtac aataccccca tcgtcaattg acatcaaata tcttgatgaa
     3361 tggagaaaag aatgggtaga ttcggagttt gataagataa ttgaacaatg gagtaaaagt
     3421 taatttcaga agtggattca ctcttgctca agagtgaatc cactaatatc atatcctaat
     3481 gatatagttt aataaaatct attctggaat cattaggctg agagcgcaat aactagcata
     3541 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga
     3601 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata
     3661 agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt
     3721 ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc
     3781 aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcaatac tgttgtaatt
     3841 catttaacat tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca
     3901 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catagtgaaa acgggggcga
     3961 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     4021 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     4081 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     4141 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     4201 tatcccatat caccagctca ccgtctttca tcgccatacg gaactctgga tgagcattca
     4261 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     4321 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     4381 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     4441 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     4501 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     4561 caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag
     4621 gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt
     4681 cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
     4741 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
     4801 aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg
     4861 atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca
     4921 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt
     4981 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc
     5041 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc
     5101 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
     5161 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
     5221 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
     5281 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     5341 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     5401 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
     5461 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     5521 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     5581 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     5641 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag
     5701 cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca tgc
//
