LOCUS       sequence-304777-        7742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the qatABCD defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG027 (qatABCD)
FEATURES             Location/Qualifiers
     source          1..7742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1317..1328
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2977..2988
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      5522..5569
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5789..6448)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6363..6382
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6449..6551)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6943..6960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7077..7622)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7157..7176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagaaat tatttggaat
      121 ggatgatggc gcttgattac tggaacaggt ctatgacatg aaggttatga tttgttcact
      181 gctatgaggt taacacttta acaatttccc ttactattct tgtactaatt ccttccaaat
      241 acttctgctt gagattagga tttatcctct tgtagtgtta tttacaataa agattgtgat
      301 gctgatttaa cccaacgtgt tgtcagttgc cttgctgaac taagttcagt atctagaaat
      361 tagctcttga tacatgagcg aatcagcgaa aattttcatc ccgaccaatt aatgaccgta
      421 atggatagga tgttgctgct atttggcttc catgagggaa catatgtttt taaacgatca
      481 agaaacgtcc actgacctgc tgtactacac cgctatcgcc agcacagtgg ttaggcttgt
      541 tgatgaaacg tcagatgcac ccattacgat tggtgtgcat ggtgattggg gggcgggaaa
      601 atcaagcgta ctaaaaatgc ttgaggctgc ctgcgagaaa aaggataaaa cgcactgtat
      661 ctggtttaac ggatggacgt ttgagggatt cgaagatgct aaaactgtaa tcatcgaaac
      721 catcgtcgag gatcttgttg cctcgcgccc gatgagcacc aaggtggcag aagcagcaaa
      781 aaaggttctt cgtcgaattg actggttgaa aatggccaag aaagcggggg gactggcgtt
      841 taccgcattt actggcatac ccacatttga tcagattaag gggatgtacg aactggcatc
      901 cgactttcta agtgctccgc aggacaagct ttctgctgca gatttcaaag cgtttgctga
      961 aaaagcagga ggcttcatca aagaggccga tactgatagt aatacgctac ccaaacatat
     1021 tcatgctttc cgtgaggagt tcagggcgct gcttgatgct gctgaaattg aaaagctagt
     1081 ggtgatcgtt gacgatcttg atcgctgcct gcctaaaacc gcgattgaaa cgctcgaagc
     1141 tattcgcctt ttcttgtttg tagagaaaac tgcatttgtt atcggtgcag atgaagccat
     1201 gatcgaatat gcggtaaaag accatttccc cgacctgcct caaagcaccg ggccggtaag
     1261 ttatgcacgc aactatcttg aaaagctcat acaggttcca tttcgaatcc ccgcactggg
     1321 aactgcagaa acgcgtatat ataccacgtt gttgcttgca gaaaatgcgt tgggttcgga
     1381 ggacgacaat tttaaagcat tgctcaataa agcacgggaa gagatgaagc gtccttggat
     1441 cagccgcggg cttgacagag aggcagtgat ggcagcgtta aatggaaaga ttccggaggt
     1501 tgtggaaaac gcgctgctat tcagcctaca cgttacccct atgcttagtt cggggacaca
     1561 tggtaatcca aggcagatta aacgcttttt gaactcaatg atgttacgcc aggcgattgc
     1621 tgatgaacgc gggttcggta gtgacattaa gcgtcctgta ctggcaaaaa ttatgcttgc
     1681 tgagcgtttt taccccagcg tatacggaaa gcttgttcag cttgtatcta atcatccaga
     1741 gggaaaaccg gaagctttgg cggagtttga agccttggtc agagggggga aaactgctcc
     1801 gaagagtcgc gctgacagca aagagaattc ctcagagtct gaagacgtcc aaaactggct
     1861 gaagattgat tgggcgatcg gttgggcaaa agcagagccc gcactttctg gagaggatct
     1921 tcgtccatat gtgtttgtca ctcgtgacaa acacagtact ttgagtaatc tggtcgtatc
     1981 aagccatctc attcctataa tggagaaact tcttggtccg aaaattggga tggtgaaaat
     2041 caaaggggat ttagagaaac tgagtccacc ggatgctgat gaattattcg aaatgcttag
     2101 cgataagctt ttccaagaag acagtttcaa tcgaaaacca agaggatttg acggcctcga
     2161 atatctcgta gaaacacaac ctcaccttca aaggagattg attgattttg cacggcgcat
     2221 tcctgtaaaa aaagcagggg gatggcttgc tacccgtatt gcgcaaagcc tagtggaccc
     2281 tacgttaata gaagaatata caaaactgat ccaagaatgg gcgagtcagg acgaaaatct
     2341 gtccctctct aaatcagcaa aagcaaccct ccagttatcg ggatatcaac attaatggga
     2401 acctcaaaag cttacggggg gcctgttcat ggcctaatcc ccgatttcgt ggagaatcca
     2461 tctccaccga ccctgccgcc tgttgaccct gcggatgata gcacgctgga tacgccgctc
     2521 attccaccgg attcgagtgg ctcagggcca cttagcacac cgaaagcaaa ctttactcga
     2581 tactcccgtt caggaagtcg tagttctctg ggtaaggcgg tcgctggata tgtccgcaat
     2641 ggagtggggg gcgcaggcag ggccagccgc cgtatggggg cctcacgcgc tgcagcaggg
     2701 ggactgctcg gtctcatcag cgactatcag cagggaggtg ctactcaggc tcttgagcgc
     2761 ttcaatcttg gtaatttggc agggcagtct gcatcgactg ctcttctctc ccttgttgaa
     2821 tttttatgcc ctccaggtgg ttctgttgac gagggggttg cgcggcaggc tatgctagag
     2881 accatcgccg atatgtctga tgtaggagag gagaattttg atgagctcac tcccgatcaa
     2941 ttaaaagaag tctttattgg tttcgtggtt cactccattg aagggaggct catggcggat
     3001 attggtaaaa atgggatcaa gttaccagac gacatagacg ctatcgtcag tatccaggag
     3061 gacctgcatg attttgttga tggagctact cgtacacagc tccgtgagga gctgaggaat
     3121 cttacagggc tttcagggga tgctatagac agaaaagtgg aggagattta caccgtggca
     3181 tttgaattac ttgcccgaga aggggagaga ttggaatgag ccatcatacc ttagttgccc
     3241 gtttgggcac tgacgataac tccgatttac agctcagccg ccaaagcacg catctgacag
     3301 aaattaattt tctcaaagag aacggtaaac tggatttcgg tctcgggcag gcgctgaatg
     3361 gtttgagtga tcttggttta acgccaatgg atgtctccgt ggatctggca ctactggccg
     3421 caacggtgac tgcggcggac acccgaatct cacgtgggca taacgctcaa gatctgtgga
     3481 cgcgcgaaat tgcactttat atcccggtag cttccccgac attatggaat agtcagactg
     3541 gattgctcag caggatgttg aattttctta ccggcgaccg ttggacaatt catttccgct
     3601 cgcgccctgt tattgagcac gggctcattc agcgatcctc taaggaacgt tcggtgaacc
     3661 ctacttctgt ttgcttgttt tccggggggc tcgacagctt catcggtgcc attgatttat
     3721 tatctaatgg gggaaccccc cttctgatca gccactactg ggatacgact accagcgttt
     3781 atcagcagaa gtgtgctcag ctgctgtcgg agcgatatgg acaatcgttc agccatgtgc
     3841 gagctcgtgt tgggtttgaa aaaacaacga ttgagggaga agatggagaa aacacccttc
     3901 gtggccgctc tttcatgttt ttctcgctcg cgacaatggc cgcagacgcc ctcggcgggc
     3961 cggtcacgat aaacgtccct gaaaatggtt tgatctctct caacgttccc ctcgatccgc
     4021 ttcgtgtcgg agcgctaagt actcggacaa cccatccgtt ttacatggcg cgttttaatg
     4081 agctgctggg caaccttggc atcagtgcac atctggaaaa tccctacgcc tacaaaacca
     4141 aaggtgagat ggctatccat tgccatgacc atgcttttct aaggcaacac gcggctgaca
     4201 ccatgtcatg ttcgtctccg caaagtacgc gttggaaccc tgcgctgaat gagcagcaat
     4261 caacacactg tggccgatgt gttccatgct taatcaggcg agcatcattg tttacagctt
     4321 tcggcacgga cgatacgatt taccgtatcc cggatctccg tagccgggta ctggacagct
     4381 ctaagcctga aggtgaacac gttcgggcat ttcaatttgc tctggcaaga ttggcgcgat
     4441 caccgagtcg agcaaaattt gatattcaca aaccagggcc gctcagcgac tatcccgact
     4501 gcttagctga gtatgaaggt gtttatctga gaggaatgaa agaagttgaa cgcctgctga
     4561 gtggagtcat aacgaggccc cttacatgaa attagcagga cagaagcccg ctccacaatg
     4621 ggtcgatttt cactgtcatc tggatctata ccccaatcac tctgcactca tccgtgaatg
     4681 tgacatttca cgtgttgcca cgctagcggt gacgacaacc cccaaggcat ggatgcgtaa
     4741 ccgggagtta acttccgatt ctccttatgt tcgtgtcgca cttggtctac atccccagct
     4801 gattgcggaa cgtgagcatg agatagcgtt actggagcac tatctccctt ctgcacgtta
     4861 cgttggggag atagggcttg atgccagccc gcgcttttat cgcagctttg aagcacagga
     4921 gcggattttt tcccgtattc tgaatgcctg tttcgagcag ggggataaga ttctcagcat
     4981 ccacagcgtt cgcgctgcag ccaaagtgtt gggacatttg gaaaacacca gacttactga
     5041 aaattgcaag gctgtcctac actggttcac tgggagtatc tccgaggctc gacgagctgt
     5101 tgaacttgga tgctatttct ctattaatga agagatgcta cgttctccta aacatcgaaa
     5161 gctggtgtcc tttttgcctt tcgaacgtat cttgacggag accgatggac cttttgtgtt
     5221 tcacgaagaa aaagcgatac accctcgtga tgtgcagcgt acggttcatg aaatcgcgca
     5281 gatccaccac gtatcggaca cagatgctgc tatgagaata ctttataatc ttcgaagttt
     5341 agtcaccaat agttctcaca gtgagaatag ttcatgaatc taattagttg gattaataca
     5401 ggggaatagt tgaatacttc agtcccctaa aagctaatat gctctatgtc atctaatgat
     5461 aagtggctcc aaagagccac ttatcattaa cttttctaaa gggaggtaga agtcgcaata
     5521 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
     5581 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     5641 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     5701 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca ccaggcgttt
     5761 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcaatact
     5821 gttgtaattc atttaacatt ctgccgacat ggaagccatc acagacggca tgatgaacct
     5881 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atagtgaaaa
     5941 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     6001 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     6061 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     6121 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     6181 ggtgaacact atcccatatc accagctcac cgtctttcat cgccatacgg aactctggat
     6241 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     6301 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     6361 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     6421 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     6481 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     6541 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     6601 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     6661 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     6721 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
     6781 cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg
     6841 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
     6901 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     6961 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     7021 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     7081 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     7141 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     7201 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     7261 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     7321 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7381 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     7441 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     7501 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     7561 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     7621 aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt
     7681 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat
     7741 gc
//
