LOCUS       sequence-30478-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Boy AL, Zhai Z, Habring-Muller A, Kussler-Schneider Y, Kaspar P, 
            Lohmann I.
  TITLE     Vectors for efficient and high-throughput construction of 
            fluorescent Drosophila reporters using the PhiC31 site-specific 
            integration system.
  JOURNAL   Genesis. 2010 Apr 22. [Epub ahead of print]
  PUBMED    20506180
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2010 Apr 22. [Epub ahead of print]"
COMMENT     Sequence Label: pHPdesteGFP
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    492..616
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(759..778)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcgtt
      301 taaacaggcc tctcgactct agcgcgtacc ctagaggatc agcttggctg cagattgttt
      361 agcttgttca gctgcgcttg tttatttgct tagctttcgc ttagcgacgt gttcactttg
      421 cttgtttgaa ttgaattgtc gctccgtaga cgaagcgcct ctatttatac tccggcgctc
      481 ctcgagatcg aaccactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata
      541 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
      601 atatccagtc actatggtcg aggtgcagac tggctgtgta taagggagcc tgacatttat
      661 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
      721 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc
      781 cagctttcat ccccgatatg ca
//
