LOCUS       sequence-304780-        5939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the TerY-P defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG030 (TerY-P)
FEATURES             Location/Qualifiers
     source          1..5939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3719..3766
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3986..4645)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4560..4579
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4646..4748)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5140..5157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5274..5819)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5354..5373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcacgtga tgaatgaagc
      121 ggctaaatac attaatgata attataattt aattcattaa aatcagtaat atataaatat
      181 aaaagttgtg aaatgtgata ttcgtcaaag catgtcaaaa agttttgact gttctttagg
      241 catcattcgc aattgtctaa caacttgata ggataggaac aatctcaaaa aggaaaatga
      301 catatggcat acgaagctca aatcagccgt actaatccag cagcatttct tttcgtcgtc
      361 gatcagtcag gttcaatgtc cgacaaaatg tcttccggcc gaagcaaggc tgagtttgtc
      421 gccgatgctc ttaatcgaac tttaatgaac ctaatcactc gctgcactaa gtctgaaggc
      481 gtacgtgatt atttcgaaat tggtgttttg ggttatggcg gtcaaggggt ttctaatggt
      541 ttctctggtt cactgggagg acaagtcctc aatccaattt ctgctctcga acagaatcca
      601 gccagagtag aagatcgcaa acggaagatg gatgatggag ctggcggaat catcgagaca
      661 gcaattaagt ttccagtatg gttcgatcct attgctagtg gcggcacgcc tatgcgtgaa
      721 gccctgacca gagccgccga agagttggtg acttggtgtg atgcccatcc ggattgctat
      781 cctccgacta tcctgcatgt gactgacggc gaatcaaacg acggtgaccc ggaagagatt
      841 gccaatcatc tacgacaaat tcgcaccaat gacggtgaag ttctgattct taatatccat
      901 gtcagttctc tcggaaatga tccaatcaga ttcccctcct cagacactgg cttaccggat
      961 gcctacgcta aactgctttt ccgtatgtcc agccctcttc cggaacatct ggtgcgtttc
     1021 gcgcaggaaa aaggtcatac ggtcggtata gaatctcgtg gattcatgtt caacgctgag
     1081 gctgccgaac tcgtcgattt cttcgacatc ggaacccgcg cttctcagtt gcgttgattc
     1141 agcaatgaaa ctggagttct tagggacagt tccgaaagat cctgaatacc ctaaggcgaa
     1201 tgaagataaa tttgccttct ccgaagatgg gagaaggctg gcgctatgtg atggcgcgag
     1261 tgagtccttc aactcaaagt tatgggccga tcttcttgct cgtaaattta ctgcagatcc
     1321 gaaagtaaat cctgaatggg tagcatctgc tttagcggaa tattctgcca cgcatgactt
     1381 cccttctatg tcctggtccc agcaagcggc attcgaaaga ggcagttttg cgacactaat
     1441 aggtgtagag gaatttgaag agcatcaggc ggtagagatt cttgctattg gagatagcat
     1501 caccatgctg gttgattgcg ggaaactcat ttgcgcatgg cctttcgata atccagaaaa
     1561 atttaatgag cggccaacac tgcttgctac gctgtacgct cataacaatt tcgtcggtgg
     1621 aagcactttc tggacacggc atgggaaaac tttttacctt gaaaaactca cccaacccaa
     1681 actcctctgt atgacagatg cgctcggcga atgggcactg aaacaagcgc tggcagagga
     1741 ttctggtttt atcgaattac tttcgctgca aactgaagaa gagcttgcag agttagttct
     1801 gagagagcgt gcagcaaaac gtatgcatat cgacgactca acgctgcttg tactatcgtt
     1861 ttaacgcgga aagtaaagat gccttaccca tctcttgaac aatacaacca agcgtttcag
     1921 ctacatagta agctgctaat cgatcctgaa ttgaaatctg gtaccgttgc cacgacaggg
     1981 ttgggtctcc ccctagccat cagcggtggc tttgcactga cctatacaat caaatcaggc
     2041 gctaagaaat acgccgttcg ttgctttcat agagagtcaa aagccttaga acgccgttat
     2101 gaggctatat ccaggaagat ttcaagcctt cgctctccct actttctcga tttccagttt
     2161 cagccccaag gggtcaaagt cgaaggaata tcatacccta tcgtcaaaat ggcatgggcc
     2221 aagggagaga cgctaggaga attccttgag gtcaacaggc gttctgcaca agcaatagcg
     2281 aaactatctg catcgattga atcacttgcc gcctaccttg aaaaagaaaa aattgcacat
     2341 ggtgatttcc agactggaaa cctgatggtc tccgacggag gtgcaaccgt ccagttaatc
     2401 gactatgacg gcatgttcgt tgatgagatt aagacattag gaagctcgga gttggggcat
     2461 gtcaattttc agcatccccg tcgtaaagca acgaatccgt tcaatcacac tctggatcgt
     2521 ttctcactaa tttcactctg gctggctctt aaagccttgc aaatcgatcc gtccatttgg
     2581 gataaatcaa attcggaact ggatgcaatc atttttcgag ctaatgactt tgtagacccc
     2641 ggttcatctt ccatcttagg gatgctatcg ggaattcaac agctttccac ccatgtaaag
     2701 aattttgccg cagtctgcgc ttcagcgatg gaaaaaacgc cttccctcgg tgacttcatt
     2761 gcaagtaaaa acattcccat atcgctagct tcgatcagta tgaatgggga tattccagtc
     2821 agcaggctga aacccggtta tatcggtgcc tacaccgtcc tgtcagcctt ggattacagt
     2881 gcttgccttc agcgagttgg tgataaagtt gaagttatcg gaaagattat tgacgtcaaa
     2941 ctcaataaga cccgaaatgg caaaccatat atctttgtta atttcggaga ttggcgcggt
     3001 aatatcttta aaatatcaat atggagtgaa ggcattagcg ctttaccttc aaaacccgat
     3061 gcctcatgga tagggaaatg gattagtgta atcggcctta tggaaccgcc ttacgttagc
     3121 gggaaataca aatattcaca tatctcaatt acagtaacga ctatcggtca aatgaccgtt
     3181 ctttcagaac cagatgcccg ctggcgtctt gctgggccaa acgaaagtcg acaaacatta
     3241 acttctacta gcagtaatca ggaagccttg gagcgcatta agagtaagag caccacttca
     3301 actcctatgc ccatgaacac taacgccaca actgcaaatc aggcaatcct taacaagtta
     3361 cgggcttcta cgcaaactgt agcggcagca agagcgcaaa ctcagcatgt agtacctaat
     3421 aaatcatcaa cgcattatgt ggcaccgacg ggaacatcag cttcgcagcc agttcaaaat
     3481 attccgagcc ctgctagtac ctcaaagcag caaacctctc aaaaaaatat agttacaaag
     3541 attttgaaat ggctttttgg atgattggta cttgtaaaga acaagcgcaa tttcagtggc
     3601 cgtatcactt gcgcttgagg tgcctgcggg tatgatcttg cgacatacac cactaaaacg
     3661 aattcgtggc ggcactttta gcctgcccct gtgttttccc gaggatttac cgcaataact
     3721 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc
     3781 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     3841 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     3901 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag
     3961 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg caatactgtt
     4021 gtaattcatt taacattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
     4081 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccata gtgaaaacgg
     4141 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     4201 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     4261 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     4321 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     4381 gaacactatc ccatatcacc agctcaccgt ctttcatcgc catacggaac tctggatgag
     4441 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     4501 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     4561 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     4621 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     4681 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     4741 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     4801 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa
     4861 gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
     4921 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
     4981 aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct tactatgttg
     5041 gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt
     5101 gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg
     5161 ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga
     5221 agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
     5281 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     5341 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
     5401 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     5461 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     5521 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     5581 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
     5641 tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     5701 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     5761 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     5821 aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa
     5881 atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt tagtcatgc
//
