LOCUS       sequence-304781-        6434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the upx defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG031 (upx)
FEATURES             Location/Qualifiers
     source          1..6434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4214..4261
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4481..5140)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5055..5074
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5141..5243)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5635..5652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5769..6314)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5849..5868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagggct gtttggttga
      121 attaaaaata cgaactaaaa ccaacaagag tcggaaaaaa cttcaaaatg ctgcttatgg
      181 ataatagtca tcttaaaaat gtacggaaaa agagactaaa atcagaaaaa catctgttat
      241 acattgactt aaagtcatca tctccgctat gagtcctcaa tccaagttga caaatgttta
      301 gccaggagtt cccgtgaacg agcatctctc tcatatggat gtacatacct tgtttgaaga
      361 aatggacgag caggctgatg gaataacgtt taaatactca tttgatgaca tagcaaagag
      421 caacgcattg gttgtcactg agtttgtcaa ttttgagcgt gacagcacgg tagctttact
      481 cgccagcctt cttactctcc cggcacacca atctcagtgt ttgcgctttg agcttctgac
      541 gagccttgca ctaattcact gcaaaggtca gcagatagca aatatcgatg acgtgaaacg
      601 ctggtatgtc actattgggg agtcgagtag tatcgttgga gaagatcctg ctgaggacgt
      661 cttcgtcgcc cttgttgata ataaaaaagg tgattaccgt gtgctagagg gggtttggga
      721 ggcggcaggt ttttatacac aattaatggt cgaaattgta tccgacatgc cggatacgca
      781 ccgctatcgc tcgctgaaac ttgctataca ggcaattctc cgtctctcag atgtcatttg
      841 tgctcgctct ggcctttatc gttttcagga aggcgcagac gaattccctg actctcttga
      901 caccgctggt cttgatgaga aaacgctctg ttcaagggta acgttgtccg agcgttctct
      961 tcgagctgag gggatcaaac ttgctgactt agcacctttc attcttgaac cttctcatat
     1021 aagtatgctt ggaaatcagg tccctgggga gggaatgctt gaacaacggc cattgctccg
     1081 cacacgcgat ggtattgtgg ttgtacttcc taccgccatg accattgcac ttcgccaggc
     1141 agtgataaca tttgcaaagc gcacagaaga attgagcgag ctagacaaag cgttagctaa
     1201 cgtctacagc cttactttct ccgagatgcc ggtcttcggt aatggaggaa ggttaagaag
     1261 actgacatgg gagaagtaca aaatgagccg aacaacgatg gtaacctcca tcgtggatgc
     1321 tggtcatttg atggtacttc agttcgtttt gccttccata cagcaatatg ccgataccgg
     1381 tttcaacaac ttgctacagc tagatgaaga gaccacgcaa tttctagata actctgttga
     1441 acaaattaca gttgacctcg ccaaacaacc cggctttcag cgtggcatcg tcgtgcgcat
     1501 tgcatgtggg tggggggcgg gttttatggg ggtccctccc caactgccag atggttgggg
     1561 atttgaatgg atgtctggtg cggactttgt ccggttcggg gcattacccg atatgtcacc
     1621 aattgccttc tggcgtgtgc aagacgcagt cgaaacgatc aggcaagctg gtgttcgatt
     1681 aatcaatatg agcggaactc tcaatcttct tgggtggata cgtgccaatg atggccatat
     1741 ggttcctcat gaccagttac cagatgaccg tatcacaccg gaacacccgc taatgttaat
     1801 gattcccacg aatttactcc gtggtatacg aatagcggca gacacaggat atgaccggca
     1861 tcgcattagt gacaacaatg gtaaatggca tcgagtgatg aggccttcgg cagaagattt
     1921 ctttcccacc gagcgtcaga gcaagtgcta cgcatcaatt gatgatcttg aagcgcaacg
     1981 gctgacctgt gtatatgagg ggcagggtaa tctttgggta acgctcgaag ctccagaaat
     2041 ggaagattgg atgctcctcg ttgagcttgc caaaatggtt cgaacatgga ttgggcggat
     2101 tggcgaggca ctggaggtct tgagtgagca accaataaaa aaatcattaa aggtgtatct
     2161 gcattttgat ggtaacgaca atatcggcag atttgatggt gagaattttt ctgatgatat
     2221 gaatacattt tggcgacttg aacgaatcca tgagcatggg gcgattcgtg tggttcttca
     2281 agatgggtat cttgcaggtt ttcgtctacc ggataaccgt gcagaacgag ctctggtgcg
     2341 cgcactcggt acggcgtttg ccacacttct tcggatgaaa gagccagtag acaaaggggt
     2401 cactgttgag cagatagcgg tgcccaatga cagagcgcgc agcttccaca taatgcaggc
     2461 ttatgacttc aaccaatatt taggccgttc actaactaaa cgtcttttag ctattgaaga
     2521 tatcgactca gccgcagccc gaattgagct agcatggcgt gctgtttcga cagatgcacc
     2581 atcacgatat cagggtaaaa aggaagttgg aaagctcctt aatgatgtgg ttgatgtgct
     2641 gatccaagac ttactaagcg aactttcaag atttgaccgt aaacagacag taatgcgatt
     2701 acttgaaaac gttgtaaagg cacgttgtga agaggcgcac tggcgtagta ctgcagcagc
     2761 ggtccttggc ttgcatgcag gagaagaggg tgtcgaagag acgatagctc aagaaatgag
     2821 ccgttatgcg ggcgcagcgt taacttcccg gctaatcatt gaacttgcca tctgtgtgtg
     2881 cccgacaagc ggtggaattg aaccttctga tatggcactc agtaaacttc ttgcacgggc
     2941 atcactgctt tttcgcatag gtggtatgtc agatgccgta cgtttcggtg ctttgcctgc
     3001 tgatattcgc atctccccct taggtgatct cctctttcgc gatgaactcg gcaaaatggt
     3061 gcttgaacca atgctttcaa aagttactaa cgaacggttt gaggaacaag cggcacaatt
     3121 cgagcaacac tatgtgaaaa ctgccggagg ggatgatgag aatagcaaac aagatagtgt
     3181 tgcggctgaa accaccgagg accaaaccga tattttcctt gcattctgga aagcagaaat
     3241 gggcttcact ctcgaggatg gaatgcgatt tatccagttc cttgagtcca tcggaataga
     3301 gcaagaatca gcaatcttcg agatgcgaag aagccaatta gcggatgctg ctaaatcggc
     3361 tgggctcgca gatgaaacta ttgatgcgtt cctcaaccag tttatcctta gcgcgcgtcc
     3421 gaaatgggat gtagtgcccg atggatttga cctttctgat atatatccct ggaggtttgg
     3481 ccgacgcctt tcagttgctg tacgtccctt gttacagatt gaagagagtc acgatccact
     3541 aattgttatc gcaccaggac tcttgaatct gtcccttaaa tacgttttcg atggcgcata
     3601 cactgggcaa tttaagcgtg acttctttcg cacagagggt atgagagaca cttggttagg
     3661 tggagcgcgg gaaggacaca cattcgaaaa aactttggag agagaacttc gtgaaatagg
     3721 ctggacagtt cgacgtggca taggctttcc tgaaattctt cgcaggaatc taccaggtga
     3781 tccgggggat attgatcttc ttgcctggcg ctcagaccgc aatcaagttc tcgttatcga
     3841 atgtaaggac ctctcacttg ctcgtaatta ctcagaagtt gcctcgcaac tatctgaata
     3901 tcaaggtgat gacataaagg gcaaaccaga taaactcaag aaacacctta aacgcgtatt
     3961 actagccaaa gaaaacatcg ataattttgc caagttcact tcgatagcga atcccgagat
     4021 tgtatcgtgg ctcgttttca gtggagcatc tcccattgcc tatgctcaat ccaagattga
     4081 ggctttggca ggaactaatg ttggccgccc aagtgatctt ctgaactttt gatagatatg
     4141 ctgtgcgata agacgccctg gcaactaagt taatcgttcc tactactgat agttttaaat
     4201 caaggcgcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     4261 gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc
     4321 ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg
     4381 gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag
     4441 caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     4501 catcgcaata ctgttgtaat tcatttaaca ttctgccgac atggaagcca tcacagacgg
     4561 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     4621 ccatagtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     4681 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     4741 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     4801 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     4861 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc atcgccatac
     4921 ggaactctgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     4981 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     5041 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     5101 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     5161 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     5221 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
     5281 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
     5341 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg
     5401 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
     5461 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac
     5521 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     5581 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     5641 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     5701 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     5761 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     5821 cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc
     5881 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     5941 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
     6001 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct
     6061 tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt
     6121 tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg
     6181 gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct
     6241 tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac
     6301 caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag atttcagtgc
     6361 aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct
     6421 catgttagtc atgc
//
