LOCUS       sequence-304782-        5400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the ppl defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG032 (ppl)
FEATURES             Location/Qualifiers
     source          1..5400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3180..3227
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3447..4106)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4021..4040
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4107..4209)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4601..4618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4735..5280)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4815..4834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagatgg actggtactg
      121 tagattcacc gtggaccagc gaatctatta tgtggtgagc agaacattaa cacatcaatg
      181 taacgccgta atcattgagt ctttgccggg gacgcttgac atctccgaaa gaattatatc
      241 gtgagtctta aggggaatct cttgcttccg gttatacatt taaccggatc tagctataag
      301 actgttacat ctattgggat taggtcagga cagatagcct gaaagctttt atagtgaggg
      361 acttcagaaa taccctagaa aaggaactgt tatggtaggt tcgcgctggt ataaatttga
      421 ttttcataac catactccgg cttcgcatga ttacaaaatt cctgacatca gccccagaga
      481 gtggcttctg gcttatatga aacagcatgt cgattgtgtt gtaatcagcg atcataacag
      541 cggagcctgg gtcgacgtgt tgaagggtga gctggagaat atgtcccggg acgccagcac
      601 cggcgacctg ccggaatttc ggccactgac actctttccg ggggttgaac tgacagcgac
      661 cggtaacgta catattctgg ctgtgctgca cacgcacagt acaagtgccg atgtggaaag
      721 gcttctggcc cagtgcaata ataatagccc cattccgagt gaagtcccta accatcagct
      781 cgttcttcaa ctgggccccg ccggcatcat cagtaatatc cgccgtaatc cgaaggctgt
      841 ttgtattctt gcgcacattg atgcagccaa aggtgtctta agtctgacta atcaggcaga
      901 gctcaccgca gcctttcagg aaagtcccca tgccgttgag attcgacacc gggtggagga
      961 tatcaccgac ggaacccgcc ggcggctgat tgataattta ccgtggctac ggggctctga
     1021 tgcgcaccat cctgaacaag ccggcgtgcg aacctgctgg ctgaaaatgt catcccctga
     1081 ttttgacgga ctcaggcatg cactgctcga tccggaaaac tgtgtgctgt ttgatcagct
     1141 ccctccggag gaacctgcgt catatttgcg cagcctgaaa ttcagaaccc gccactgcca
     1201 tcctgtgggt caggattcgg cctcggtgga attcagcccg ttctataacg ctgtaatcgg
     1261 ctcaagaggc agcgggaagt ccacgctcat tgaaagcatt cgtcttgcaa tgcgcaaaac
     1321 agaaggtctc actgcgaccc aggggagtaa gctggaccag ttcattcgga cggggatgga
     1381 agcggattcc ttcatcgaat gtattttcca caaagaaggc acagatttcc ggctcagttg
     1441 gcgaccagac agtaagcatg aattacatat cttcagtgac ggagaatgga tgcctgacag
     1501 tcactggtcg gctgaccgtt ttccactctc gatttacagc cagaaaatgc tctatgagct
     1561 ggcttcggat actggtgcat tcctgcgcgt ctgtgatgag agcccggtgg ttaacaaacg
     1621 ggcctggaaa gagcgctggg atcagctgga aagggaatat ctgaatgaac aaatcacgtt
     1681 gcggggcctg cgtgccagac agggaagtgc ggattcgctg cggggggaat tatcggatgc
     1741 tgaacgtgcc gtcagtcagc tgcagtcaag cgcctattat ccggtttgca gacagctggc
     1801 cctcgccaga aacgagctgt ccgcagcaac cttacccctg gagcactttg agcggcgtat
     1861 tgcagccatt caggctctgg cagaagaacc gctgcagaga tccgatatcc cgccggaacc
     1921 ttccggtctg ctgatggcat ttatggcgcg cctgtcatct gtgcaacagc agtatgacca
     1981 gcggctcaat actctcctgg cagaatatgc tgcagagctc gcgggtatca ggagagagca
     2041 atcttttatt gccctccgaa cagcagtgag tgaccaggaa acaaatgtag aaagtgaagc
     2101 tgtttccctg cgggccagag ggcttaatcc cgatgttctc aacgaactga tggcacgctg
     2161 tgagtcactg aaaaatgagc tgagaaatta cgacggtctt gatggggcga tctctgcctc
     2221 tgttgcacgg tctgagcagt tgctggctga aatgcgtgcc cacagaatgg cattgacaga
     2281 taaccggaag gcgtttctct cctccctgtc gctcagcgct ctggaaatca aaattcttcc
     2341 cctctgcgcc ccttatgaag atgttatatc tggttaccag acggttaccg gcatcagtaa
     2401 ttttgccgaa cgtatctacg ataacagtga cgggagcgga ttactgagcg actttatcag
     2461 tgaacgtccg ttcagcccgt tgcctgccgc aacagagaaa aaatacaggg cgctggacga
     2521 gctgaaagcg ctgcatcaca gcatccggct ggataattca gaggctgggg cggggcttca
     2581 tggttctttc cggaatcgtc tcaggagtct gaatgaccag cagctggatg ccctgcaatg
     2641 ctggtatcct gatgacggca tccacatacg ttaccagacc cccggggggc agatggaaga
     2701 cattgccttt gcttctccgg ggcaaaaggg agcgagtatg ctgcagttcc tcttatccta
     2761 tggcaccgat cctctactac tggatcaacc ggaggatgac ctggactgcc tgatgctgag
     2821 catgagcgtg atccctgcca tcatgtcgaa caagaaacgc cggcagctga ttatcgtgtc
     2881 gcactctgcc cctatagtgg ttaacggcga tgcagaatat gttatcagta tgcagcacga
     2941 tcgcacaggc ctgtatccag gactctgcgg tgcactgcag gaagctccga tgaaggcact
     3001 gatatgccgt caaatggagg ggggagaaaa agcgtttcgt tcgcgctatg agcgtattct
     3061 tagctgaaga acggaaccgt ccttaaggcg gccatgaccg gagagtgggc ctggcggctg
     3121 aatgcctgga taaaagacgc aaatgtcaga ctgatggcct ctgcgtcttt gcgcaataac
     3181 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg
     3241 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
     3301 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
     3361 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa
     3421 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcaatactgt
     3481 tgtaattcat ttaacattct gccgacatgg aagccatcac agacggcatg atgaacctga
     3541 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat agtgaaaacg
     3601 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     3661 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     3721 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     3781 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     3841 tgaacactat cccatatcac cagctcaccg tctttcatcg ccatacggaa ctctggatga
     3901 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     3961 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     4021 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     4081 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     4141 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     4201 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     4261 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     4321 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     4381 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     4441 taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt
     4501 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     4561 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     4621 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     4681 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     4741 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     4801 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     4861 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     4921 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     4981 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5041 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     5101 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     5161 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     5221 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     5281 gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca
     5341 aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc
//
