LOCUS       sequence-304783-        6010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the ietAS defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG033 (ietAS)
FEATURES             Location/Qualifiers
     source          1..6010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3790..3837
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4057..4716)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4631..4650
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4717..4819)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5211..5228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5345..5890)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5425..5444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaataga acgatgaagg
      121 atggaagcta catattctcg gtactaagat ttatttttct gacacaaaat gaccatttgg
      181 cgttacataa tcccaaaaaa acgtatcaaa aatctcaaaa tgcgttacga ttagagagta
      241 ttttgattct gcgtgctcat tttttgattg ctgtggcttt ttgttgtggg agtgttgaat
      301 ggattattta tcagaagtgt taaaaatcat tgaaggtgca acaaaggcaa atgcttcgat
      361 ggctagtaat tatgctgggt tgctggcaga taagctcgaa caaaaagggg aggtcaagca
      421 agccagaatg ataagagaaa ggttgcttag agctccccag gcgttggcag gagctcaaag
      481 ggctggaggt gggatatctc tgggctcatt accggtagat attgatagtc gactcaacac
      541 tgttgatgtc agttatccta aattagacag ttcagagatt tttctgcctg cagcaatcag
      601 tacccgtgtt gaagagttta tcactaatgt tcaacgttat gatgagtttg ttaaagctga
      661 tgcagcattg ccgagtcgta tgctcgtgta tggaaagcca ggaacaggta agactatgtt
      721 atctaagtac atcgctaccc gcttagattt tccacttctt acagtgcgtt gcgatacttt
      781 gattagtagt ttattgggac aaaccagcaa aaatcttaga caggttttcg attatgtaat
      841 gcagaggcca tcagtgcttt ttttagacga atttgatgct ttagctggag caagaggtaa
      901 tgagagagat ataggtgagc ttcagcgagt tgtcatttca ctattgcaga atatggatgc
      961 ggcatcagag gatacggtaa ttattgcctc aactaaccat gagcaacttc tggatcctgc
     1021 aatctggagg cgatttagct tcagaattcc aatgcctctg cctgacatac atcagagaga
     1081 gttaatttgg aaaaatcgtt taaagaatat gatatgtagc gatctagatt taagtgattt
     1141 atcaagaaaa tcggaaggat tatccggagc aataattgaa caggtgagct tggatgcacg
     1201 tagggatgca gttattgaag gtgcaagtgt gataaatcac cataaattgt ataggcgttt
     1261 gtatcttgct caatcgctta tggaaggtgt aaatttaagc acttacgaag atgaaattcg
     1321 ttggttacgt tctaaagata aaaaattatt ttctatcaga gttcttgcta atttgtacaa
     1381 acttacatca agagtaattt caaacattct gaaggagtca ggagcatatg agcagaaggg
     1441 gtacacagtt tagtaacgca aaagttacaa acccaatgtt aagaatccct ttttccagta
     1501 gtgacttggg tgcaatagta aacgctggcg gtggggcaaa ggtattggtt gatgtaacag
     1561 ccgaatatag acaagggcta gtaagaaatt taacaaccag taaacattat ttagaatcca
     1621 aactttcaga gtaccctgga agcttgggta ctttggtttt caaattaaga gaccagggaa
     1681 tagccaaaac gcataggccg aacaaaattg ctcaagaggc tggattgcaa aatgccggtc
     1741 atgccaaaat agatgaaatg ttggttgctg ctcatgccgg ctgttttgac gtattagagt
     1801 cagtcatttt acatcggaat attaaagcga ttttggctaa tctaagcgcg attgagcgca
     1861 ttgaaccttg ggatgagaat aggaaggttc caggaggcac tgatggtttg tttgaatcat
     1921 caaacatcct tgtacgacta tttgagtaca caggtgaaga tgcaacttac aacaactatg
     1981 aaaacgttat ttctatatta gaacaacacg gagttaaata tgatgagatt agacaaaaat
     2041 gtggtcttcc cttattaagg ataatggatt tatccccaaa tgatagatat atattagaca
     2101 ttctcattga ttacccgggt ataagaacgt taattcctga accaaaatat tcagcattcc
     2161 cggttagtgt aagtgattct gttggcattg aaacaaatag ctttcccgta ccatcagaag
     2221 aattacccat tgttgctgta tttgacactg gggtaagccc catcgcggca acaattactc
     2281 cttgggtagt gagtagggaa acatacgtaa ttcctcctga tacgagttat gaacatggga
     2341 ctatggtgtc ttcattgata tcaggcgctc attttttaaa tgacaatcat ccatggattc
     2401 ctgatacaaa atctaaaatc catgatgttt gtgccttaga tgaaaatgga tcttatatat
     2461 cagatttaat tctgaggcta gcagatgctg taaataaaag accagatata aaagtctgga
     2521 atttgtcttt gggaggcgga ccatgtaatg agcagacgtt tagtgatttt gcgatggagt
     2581 tagatcggct cagcgataaa tttggtattt tgtttgtagt tgctgcaggt aattatgtag
     2641 atgaacctat acgtacatgg ccaaatcctg atccgcttgg aggtgctgat ttaatttcct
     2701 ctcctggaga gtcagtccga gcactaacag ttggttcagt ttctcatatg gaagctaatg
     2761 atgctttaag tgaaattgga acaccgacac catatactcg tcgtggccct gggcctgtat
     2821 ttactccaaa gccagatata atccatgctg gcggtggggt tcatagacct tggaatgtag
     2881 gagcaagcag tttaaaggtc gtagggccag ataataggct ttgctctaat tttggtacta
     2941 gttttgctgc tccaattgtg gcaagtttag ctgcgcatac atggcagaga atagccacta
     3001 atacagactt taatgtttca ccatcattga ttaaagcatt attaattcat tccgctcaat
     3061 tatcttctcc tgattactcg ccaagtgaaa gacgctattt gggagcggga attcctaatg
     3121 aagttattga gaccttatat gatagtgatg ataggtttac tctgattttc caaacattct
     3181 tggttcctgg ggtgaggtgg agaaaggata actatcccat accatcggca cttattcaaa
     3241 atggaaaatt taaaggtgag attgtaatta ctgctgcata tgcaccacca ctgaacccta
     3301 atgccggcag tgaatatgtt cgcgcgaacg tagagctaag ttttggctta attgagaata
     3361 atactataaa aggaaaagtg cctatggaag gagaaaacgg tcaatctgga tatgagagag
     3421 ctcaaattga gcatggtgga aagtggtcac cagtaaaaat tcatcgcaag gcatttaata
     3481 aaggaattac ttcgggtaac tgggctcttc aggctaaaac aacgttgaga gcgaatgaac
     3541 cggccttaat ggagccttta cctgtaacta ttgtagtaac tttaaaatca ttagatggaa
     3601 acacacaagt ttatgctgat ggcgtaagag ctttaaatgc taataactgg gctcactatc
     3661 cattgcctgc tcgtgtgcca gtttccgtat aacaactata taaatcaaac ccgctgtagc
     3721 gggtttgatt tatttgtggg tgtgttttat aaaaataccg cccatacaca acaaaataca
     3781 acgcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     3841 aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     3901 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
     3961 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc
     4021 aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc
     4081 gcaatactgt tgtaattcat ttaacattct gccgacatgg aagccatcac agacggcatg
     4141 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     4201 agtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     4261 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     4321 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     4381 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     4441 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcatcg ccatacggaa
     4501 ctctggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     4561 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     4621 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     4681 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     4741 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     4801 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
     4861 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
     4921 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt
     4981 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
     5041 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc
     5101 ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag
     5161 gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact
     5221 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
     5281 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
     5341 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
     5401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg
     5461 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
     5521 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
     5581 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5641 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
     5701 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
     5761 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
     5821 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
     5881 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt
     5941 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg
     6001 ttagtcatgc
//
