LOCUS       sequence-304786-       10082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A S. pneumoniae not integrated, temperature sensitive plasmid that 
            carries the CRISPR system; a Zn inducible wtCas9 and a sgRNA which 
            has its spacer sequence replaced by gfp gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Synefiaridou D, Veening JW
  TITLE     Harnessing CRISPR-Cas9 for Genome Editing in Streptococcus 
            pneumoniae D39V.
  JOURNAL   Appl Environ Microbiol. 2021 Feb 26;87(6). pii: AEM.02762-20. doi: 
            10.1128/AEM.02762-20. Print 2021 Feb 26.
  PUBMED    33397704
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2021-02-26"; volume: "87(6). pii: 
            AEM.02762-20. doi"; pages: " 10.1128/AEM.02762-20. Print 2021 Feb 
            26"
COMMENT     Sequence Label: pDS05
FEATURES             Location/Qualifiers
     source          1..10082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      781..1519
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        1567..1658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1659..2450
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             2703..6809
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSNNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVRKDLIVKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATFIHQSITGLYETRIDLSQLGGD"
     CDS             join(7029..7031,7032..7745)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             9367..9378
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa
       61 ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa
      121 gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac
      181 agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt
      241 caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggagt
      301 attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct
      361 gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca
      421 ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa
      481 tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg ccataccaca
      541 gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga
      601 gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta
      661 aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta
      721 tttaacggga ggaaataatt ctatgagtcg cttttgaatc tagagcggtt cagtagaaaa
      781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atcttttgcc ctgtaaacga
      841 aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa ctgcttaacc
      901 gtggtaactg gctttcgcag agcacagcaa ccaaatctgt ccttccagtg tagccggact
      961 ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg cgaactccca
     1021 gttaccaatg gctgctgcca gtggcgtttt accgtgcttt tccgggttgg actcaagtga
     1081 acagttaccg gataaggcgc agcagtcggg ctgaacgggg agttcttgct tacagcccag
     1141 cttggagcga acgacctaca ccgagccgag ataccagtgt gtgagctatg agaaagcgcc
     1201 acacttcccg taagggagaa aggcggaaca ggtatccggt aaacggcagg gtcggaacag
     1261 gagagcgcaa gagggagcga cccgccggaa acggtgggga tctttaagtc ctgtcgggtt
     1321 tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc accggaaaag
     1381 cgtctgttta tgtgaactct ggcaggaggg cggagcctat ggaaaaacgc caccggcgcg
     1441 gccctgctgt tttgcctcac atgttagtcc cctgcttatc cacggaatct gtgggtaact
     1501 ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg agcgtagcga ccgagtgagc
     1561 tagctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1621 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagggaagcg gtgatcgccg
     1681 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     1741 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     1801 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     1861 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     1921 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     1981 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     2041 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     2101 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     2161 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     2221 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     2281 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     2341 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     2401 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa
     2461 ttcgttcaag tacaagcact ttgggacgtt ctcccttagt gcttttttga tttctcatag
     2521 gccggcctgt tagtcatatg gacacttaag gcaaattgtt cagaactgaa taaagctgac
     2581 gttttgcttc tatcctttct ttgagtttta gtggataatg ataatgaaca aggtgttcat
     2641 aaatctatta taacaagaat tccaatcccg ggtaaagctt ccagcggccg caggaggcaa
     2701 aaatggataa gaaatactca ataggcttag atatcggcac aaatagcgtc ggatgggcgg
     2761 tgatcactga tgattataag gttccgtcta aaaagttcaa ggttctggga aatacagacc
     2821 gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga gagacagcgg
     2881 aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag aatcgtattt
     2941 gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt ttctttcatc
     3001 gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat cctatttttg
     3061 gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat catctgcgaa
     3121 aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg gccttagcgc
     3181 atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct gataatagtg
     3241 atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt gaagaaaacc
     3301 ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg agtaaatcaa
     3361 gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc ttatttggga
     3421 atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt gatttggcag
     3481 aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat aatttattgg
     3541 cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta tcagatgcta
     3601 ttttactttc agatatccta agattaaata gtgaaataac taaggctccc ctatcagctt
     3661 caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa gctttagttc
     3721 gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa aacggatatg
     3781 caggttatat tgatggggga gctagccaag aagaatttta taaatttatc aaaccaattt
     3841 tagaaaaaat ggatggtact gaggaattat tggcgaaact aaatcgtgaa gatttgctgc
     3901 gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg ggtgagctgc
     3961 atgctattct gagaagacaa gaagactttt atccattttt aaaagacaat cgtgagaaga
     4021 ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg cgtggcaata
     4081 gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg aattttgaag
     4141 aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca aactttgata
     4201 aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag tattttacgg
     4261 tttataacga attgacaaag gtcaaatatg ttactgaggg aatgcgaaaa ccagcatttc
     4321 tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat cgaaaagtaa
     4381 ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat agtgttgaaa
     4441 tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat ttgctaaaaa
     4501 ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta gaggatattg
     4561 ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt aaaacatatg
     4621 ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat actggttggg
     4681 gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc aaaacaatat
     4741 tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg atccatgatg
     4801 atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa ggcgatagtt
     4861 tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt attttacaga
     4921 ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca gaaaatatcg
     4981 ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat tcgcgtgagc
     5041 gtatgaaacg tattgaagaa ggtatcaaag aattaggaag tcagattctt aaagagcatc
     5101 ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc caaaatggaa
     5161 gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat gatgtcgatc
     5221 acattgttcc acaaagtttc attaaagacg attcaataga caataaggtc ttaacgcgtt
     5281 ctgataaaaa tcgtggtaaa tcgaataacg ttccaagtga agaagtagtc aaaaagatga
     5341 aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag tttgataatt
     5401 taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt atcaaacgcc
     5461 aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat agtcgcatga
     5521 atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt accttaaaat
     5581 ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt gagattaaca
     5641 attaccatca tgcccatgat gcgtatctta atgccgtcgt tggaactgct ttgattaaga
     5701 aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat gatgttcgta
     5761 aaatgattgc taagtctgag caggaaatag gcaaagcaac cgcaaaatat ttcttttact
     5821 ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag attcgcaaac
     5881 gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa gggcgagatt
     5941 ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag
     6001 tacagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg gacaagctta
     6061 ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt ccaacggtag
     6121 cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg
     6181 ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa aatccgattg
     6241 actttttaga agctaaagga tataaggaag ttagaaaaga cttaatcgtt aaactaccta
     6301 aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt gccggagaat
     6361 tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta tatttagcta
     6421 gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa ttgtttgtgg
     6481 agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt tctaagcgtg
     6541 ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa catagagaca
     6601 aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg aatcttggag
     6661 ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat acgtctacaa
     6721 aagaagtttt agatgccact tttatccatc aatccatcac tggtctttat gaaacacgca
     6781 ttgatttgag tcagctagga ggtgactaag tcgacctcga gactagtcaa ggtcggcaat
     6841 tctgcagtac taggacgccg ccaagccagc ttaaacccag ctcaatgagc tgggtttttt
     6901 gtttgttaaa aatgaagaag aaactgtgaa gcgtatttag cgtataagag acagccattc
     6961 tacagtttat tcttgacatt gcactgtccc cctggtataa taactatatg agacctagga
     7021 ggacaaacat gtcaaaagga gaagagctgt tcacaggtgt tgtgccgatt ctcgttgagc
     7081 ttgacggaga tgtaaacgga cacaaattct ctgttcgcgg tgaaggtgaa ggagatgcaa
     7141 caaacggcaa gctgacattg aagtttattt gcacaactgg aaagctgccg gttccttggc
     7201 cgacacttgt aacgacgctg acttacggcg ttcaatgctt ctctcgttat ccagaccaca
     7261 tgaaacgcca tgatttcttc aaatctgcaa tgcctgaagg ctacgttcaa gagcgtacga
     7321 tcagcttcaa agatgacgga acgtacaaaa caagagcaga agtgaagttt gaaggtgaca
     7381 cacttgtgaa ccgcattgaa ttgaaaggca ttgatttcaa agaagatgga aacatccttg
     7441 gacacaaact tgaatacaac ttcaacagcc acaacgtata catcactgct gacaaacaaa
     7501 aaaacggcat caaagcaaac ttcaaaatcc gtcataacgt agaggacggt tctgttcagc
     7561 ttgctgatca ttatcagcaa aatacaccga tcggtgacgg cccggttctt cttcctgata
     7621 accattattt atcaactcaa agcgtattat caaaagaccc aaatgaaaag cgtgaccaca
     7681 tggtgctgct tgaatttgtg acagctgctg gtatcactca cggcatggat gagctttata
     7741 agtaaggtct cggtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag
     7801 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttctgtc tcaatcgcaa
     7861 catcaaacca aaataaaaac ccccttcgac tttcgtcagg gggcttttat ttattcaata
     7921 atccctcctc tcaataaatc tattgttgta cttaattcaa cttccatttc tctgtatctt
     7981 tcaatacgct cttttaagtc cttaatttct ttttttaatt cctcattttc agcaaataac
     8041 tctttttctt tgtttgtcat tttatttccc ccgtttcagc atcaagaacc tttgcataac
     8101 ttgctctata tccacactga taattgccct caaaccataa tctaaaggcg ctagagtttg
     8161 ttgaaacaat atcttttaca tcattcgtat ttaaaattcc aaactccgct cccctaaggc
     8221 gaataaaagc cattaaatct tttgtattta ccaaattata gtcatccact atatctaaaa
     8281 gtaaattctt caattctctt ttttggcttt catcaagtgt tatatagcgg tcaatatcaa
     8341 aatcattaat gttcaaaata tcttttttgt cgtatatatg tttattctta gcaatagcgt
     8401 cctttgattc atgagtcaaa tattcatatg aacctttgat ataatcaagt atctcaacat
     8461 gagcaactga actattcccc aattttcgct taatcttgtt cctaacgctt tctattgtta
     8521 caggatttcg tgcaatatat ataacgtgat agtgtggttt tttatagtgc tttccatttt
     8581 gtataatatt actattattc catgtatctt tatctttttt ttcgtccata tcgtgtaaag
     8641 gactgacagc catagatacg cccaaactct ctaatttttc tttccaatca ttaggaattg
     8701 agtcaggata taataaaaat ccaaaatttc tagctttagt atttttaata gccatgatat
     8761 aattacctta tcaaaaacaa gtagcgaaaa ctcgtatcct tctaaaaacg cgagctttcg
     8821 cttatttttt ttgttctgat tcctttcttg catattcttc tatagctaac gccgcaaccg
     8881 cagattttga aaaacctttt tgtttcgcca tatctgttaa ttttttatct tgctcttttg
     8941 tcagagaaat cataactctt tttttcgatt ctgaaatcac catttaaaaa actccaatca
     9001 aataatttta taaagttagt gtatcacttt gtaatcataa aaacaacaat aaagctactt
     9061 aaatatagat ttataaaaaa cgttggcgaa aacgttggcg attcgttggc gattgaaaaa
     9121 ccccttaaac ccttgagcca gttgggatag agcgtttttg gcacaaaaat tggcactcgg
     9181 cacttaatgg ggggtcgtag tacggaagca aaattcgctt cctttccccc catttttttc
     9241 caaattccaa atttttttca aaaattttcc agcgctaccg ctcggcaaaa ttgcaagcaa
     9301 tttttaaaat caaacccatg agggaatttc attccctcat actcccttga gcctcctcca
     9361 accgaaatag aagggcgctg cgcttattat ttcattcagt catcggcttt cataatctaa
     9421 cagacaacat cttcgctgca aagccacgct acgctcaagg gcttttacgc tacgataacg
     9481 cctgttttaa cgattatgcc gataactaaa cgaaataaac gctaaaacgt ctcagaaacg
     9541 attttgagac gttttaataa aaaatcggat aataaatata tataaacgta tatagatttc
     9601 ataaagtcta acacactaga cttatttact tcgtaattaa gtcgttaaac cgtgtgctct
     9661 acgaccaaaa ctataaaacc tttaagaact ttcttttttt acaagaaaaa agaaattaga
     9721 taaatctctc atatctttta ttcaataatc gcatccgatt gcagtataaa tttaacgatc
     9781 actcatcatg ttcatattta tcaggatcct ctagagtcgc gcaaaagaaa aacgaaatga
     9841 tacaccaatc agtgcaaaaa aagatataat gggagataag acggttcgtg ttcgtgctga
     9901 cttgcaccat atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa aagaagttat
     9961 ggaaataaga cttagaagca aacttaagag tgtgttgata gtgcagtatc ttaaaatttt
    10021 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagtgatt
    10081 ac
//
