LOCUS       sequence-30479-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Boy AL, Zhai Z, Habring-Muller A, Kussler-Schneider Y, Kaspar P, 
            Lohmann I.
  TITLE     Vectors for efficient and high-throughput construction of 
            fluorescent Drosophila reporters using the PhiC31 site-specific 
            integration system.
  JOURNAL   Genesis. 2010 Apr 22. [Epub ahead of print]
  PUBMED    20506180
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2010 Apr 22. [Epub ahead of print]"
COMMENT     Sequence Label: pHPdestmCherry
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    317..441
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(482..787)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(584..603)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtggc gaccggtgga
      121 tcgtttaaac aggcctctcg actctagcgc gtaccctaga ggatcagctt ggctgcagat
      181 tgtttagctt gttcagctgc gcttgtttat ttgcttagct ttcgcttagc gacgtgttca
      241 ctttgcttgt ttgaattgaa ttgtcgctcc gtagacgaag cgcctctatt tatactccgg
      301 cgctcctcga gatcgaacca ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat
      361 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      421 acaacatatc cagtcactat ggtcgaggtg cagactggct gtgtataagg gagcctgaca
      481 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
      541 gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca
      601 tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg
      661 acagcagacg tgcactggcc agggggatca ccatccgtcg gccgggcgtg tcaataatat
      721 cactctgtac atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa
      781 actgcatttc accagcccct gttctcgtca gc
//
