LOCUS       sequence-30480-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Boy AL, Zhai Z, Habring-Muller A, Kussler-Schneider Y, Kaspar P, 
            Lohmann I.
  TITLE     Vectors for efficient and high-throughput construction of 
            fluorescent Drosophila reporters using the PhiC31 site-specific 
            integration system.
  JOURNAL   Genesis. 2010 Apr 22. [Epub ahead of print]
  PUBMED    20506180
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2010 Apr 22. [Epub ahead of print]"
COMMENT     Sequence Label: pHPdestVenus
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    232..356
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(397..702)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(499..518)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tctcgccctt gctcaccatg gtggcgaccg gtggatcgtt taaacaggcc tctcgactct
       61 agcgcgtacc ctagaggatc agcttggctg cagattgttt agcttgttca gctgcgcttg
      121 tttatttgct tagctttcgc ttagcgacgt gttcactttg cttgtttgaa ttgaattgtc
      181 gctccgtaga cgaagcgcct ctatttatac tccggcgctc ctcgagatcg aaccactttg
      241 tacaagaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
      301 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggtcg
      361 aggtgcagac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
      421 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
      481 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
      541 caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
      601 gatcaccatc cgtcggccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
      661 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
      721 cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat
      781 tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta
      841 ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac
      901 tgtcactgta atacgctgc
//
