LOCUS       sequence-304808-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gateway donor vector encoding ratiometric sensor of lysosomal 
            lumenal pH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Ponsford AH, Ryan TA, Raimondi A, Cocucci E, Wycislo SA, Frohlich F,
            Swan LE, Stagi M
  TITLE     Live imaging of intra-lysosome pH in cell lines and primary neuronal
            culture using a novel genetically encoded biosensor.
  JOURNAL   Autophagy. 2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858.
  PUBMED    32515674
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858."
COMMENT     Sequence Label: pDONOR223  R2pH-LAMP1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..139
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(377..395)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ccgacgttgt aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
       61 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
      121 ttgtacaaaa aagcaggctg ggaattcacc atggcggccc ccggcgcccg gcggccgctg
      181 ctcctgctgc tgctggcagg ccttgcacat ggcgcctcag cagctagcgg tatggtgagc
      241 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      301 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      361 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
      421 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      481 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      541 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
      601 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcataag
      661 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
      721 gacgagatca agcagaggct gaagctgaat gacagcggcc actacgacgc tgaggtcaag
      781 accacctact aggcccagaa gcccgtgcag ctgctcggcg ccgacaacgt caacatcaag
      841 ttgtacgtca cctca
//
