LOCUS       sequence-304809-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gateway donor vector encoding ratiometric sensor of lysosomal 
            lumenal pH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Ponsford AH, Ryan TA, Raimondi A, Cocucci E, Wycislo SA, Frohlich F,
            Swan LE, Stagi M
  TITLE     Live imaging of intra-lysosome pH in cell lines and primary neuronal
            culture using a novel genetically encoded biosensor.
  JOURNAL   Autophagy. 2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858.
  PUBMED    32515674
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858."
COMMENT     Sequence Label: pDONOR223  R2pH-LAMP1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             619..684
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(695..794)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(811..830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(812..830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccaccacttg tgcccacaaa ccccactgta tccaagtaca atgttactgg taacaacgga
       61 acctgcctgc tggcctctat ggcactgcaa ctgaatatca cctacctgaa aaaggacaac
      121 aagacggtga ccagagcgtt caacatcagc ccaaatgaca catctagtgg gagttgcggt
      181 atcaacttgg tgaccctgaa agtggagaac aagaacagag ccctggaatt gcagtttggg
      241 atgaatgcca gctctagcct gtttttcctg caaggagtgc gcttgaatat gactcttcct
      301 gatgccctag tgcccacatt cagcatctcc aaccattcac tgaaagctct tcaggccact
      361 gtgggaaact catacaagtg caacactgag gaacacatct ttgtcagcaa gatgctctcc
      421 ctcaatgtct tcagtgtgca ggtccaggct ttcaaggtgg acagtgacag gtttgggtct
      481 gtggaagagt gtgttcagga tggtaacaac atgttgatcc ccattgctgt gggcggtgcc
      541 ctggcagggc tggtcctcat cgtcctcatt gcctacctca ttggcaggaa gaggagtcac
      601 gccggctatc agaccatcga ctacaaagac catgacggtg attataaaga tcatgacatc
      661 gactacaagg atgacgatga caagtaggaa ttccacccag ctttcttgta caaagttggc
      721 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      781 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
      841 gctttccgca t
//
