LOCUS       sequence-30481-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, Abell
            A, Johnson GL, Hahn KM, Danuser G
  TITLE     Coordination of Rho GTPase activities during cell protrusion.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):99-103.
  PUBMED    19693013
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):99-103."
COMMENT     Sequence Label: pBabe-Ypet-PBD
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(30..48,49..71,72..90,91..113,114..132,133..155,
                     156..174,175..197,198..216,217..239,240..258,259..281,
                     282..300)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        333..371
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     368..392
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgaggccctt tcgtcgtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
       61 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      121 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      181 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      241 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      301 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
      361 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      421 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccagatcc gtaaagtgaa
      481 ttctagcacc atggtgagca agggcgaaga gctgttcacc ggcgtggtgc ccatcctggt
      541 ggagctggac ggcgacgtga acggccacaa gttcagcgtg agcggcgagg gcgagggcga
      601 cgccacctac ggcaagctga ccctgaagct gctgtgcacc accggcaagc tgcccgtgcc
      661 ctggcccacc ctggtgacca ccctgggcta cggcggtgc
//
