LOCUS       sequence-304812-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses ratiometric sensor of lysosomal lumenal pH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Ponsford AH, Ryan TA, Raimondi A, Cocucci E, Wycislo SA, Frohlich F,
            Swan LE, Stagi M
  TITLE     Live imaging of intra-lysosome pH in cell lines and primary neuronal
            culture using a novel genetically encoded biosensor.
  JOURNAL   Autophagy. 2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858.
  PUBMED    32515674
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858."
COMMENT     Sequence Label: pCAG R2pH-LAMP1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             596..661
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cactgtatcc aagtacaatg ttactggtaa caacggaanc tgcctgctgg cctttatggc
       61 actgcaactg aatatcacct acctgaaaaa ggacaacaag acggtgacca gagcgttcaa
      121 catcagccca aatgacacat ctagtgggag ttgcggtatc aacttggtga ccctgaaagt
      181 ggagaacaag aacagagccc tggaattgca gtttgggatg aatgccagct ctagcctgtt
      241 tttcctgcaa ggagtgcgct tgaatatgac tcttcctgat gccctagtgc ccacattcag
      301 catctccaac cattcactga aagctcttca ggccactgtg ggaaactcat acaagtgcaa
      361 cactgaggaa cacatctttg tcagcaagat gctctccctc aatgtcttca gtgtgcaggt
      421 ccaggctttc aaggtggaca gtgacaggtt tgggtctgtg gaagagtgtg ttcaggatgg
      481 taacaacatg ttgatcccca ttgctgtggg cggtgccctg gcagggctgg tcctcatcgt
      541 cctcattgcc tacctcattg gcaggaagag gagtcacgcc ggctatcaga ccatcgacta
      601 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
      661 gtaggaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
      721 tgccctggct cacaaatacc actgagatct ttttncct
//
