LOCUS       sequence-304826-       14934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of hyeA3A-BE4max.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14934)
  AUTHORS   Zhang X, Chen L, Zhu B, Wang L, Chen C, Hong M, Huang Y, Li H, Han 
            H, Cai B, Yu W, Yin S, Yang L, Yang Z, Liu M, Zhang Y, Mao Z, Wu Y, 
            Liu M, Li D
  TITLE     Increasing the efficiency and targeting range of cytidine base 
            editors through fusion of a single-stranded DNA-binding protein 
            domain.
  JOURNAL   Nat Cell Biol. 2020 Jun;22(6):740-750. doi: 
            10.1038/s41556-020-0518-8. Epub 2020 May 11.
  PUBMED    32393889
REFERENCE   2  (bases 1 to 14934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-020-0518-8"; journalName: "Nat Cell Biol"; date: 
            "2020-06"; volume: "22"; issue: "6"; pages: "740-750"
COMMENT     Sequence Label: Lenti-117G-hyeA3A-BE4max
FEATURES             Location/Qualifiers
     source          1..14934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1985..2233
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1985..2005
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2156..2175
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2255..2330
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2387..2598
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2664..2684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3813..3836,3837..3839,3840..7913)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3813..7913
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7914..7961
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7971..8024
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8025..8273
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8304..8552
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8595..8615
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8625..8681
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8682..8684,8685..8687,8688..9401)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8682..8684,8685..8687,8688..9398)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8988..9007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9335..9356
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9408..9996
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9461..9481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9879..9890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10068..10301
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10327..10344)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10333..10557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10603..11031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10690..10709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10900..10921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11040..11060)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11045..11374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11225..11360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11287..11306
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11422..11469
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11488..11862
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11992..12113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12029..12048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12083..12102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12162..12178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12162..12178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12175..12197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12186..12202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12210..12216,12217..12234,12235..12240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12255..12276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12393..12410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12564..13152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12644..12663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13323..14114,14115..14183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13946..13965
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14184..14288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14363..14382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        14554..14933
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taatgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     2041 agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     2101 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     2161 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     2221 aggacgaaac accggctatt ggtcaaggca aggcgtttta gagctagaaa tagcaagtta
     2281 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgct
     2341 agctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
     2401 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga
     2461 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     2521 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     2581 tttgccgcca gaacacaggg gcgcgcctct agagcgatcg cgccaccatg aagaggaccg
     2641 ccgatggctc tgagttcgag agccccaaga agaagcggaa ggtggaggca tctccagcaa
     2701 gcggaccaag gcacctgatg gacccccaca tcttcacctc taactttaac aatggcatcg
     2761 gcaggcacaa gacatacctg tgctatgagg tggagcgcct ggacaacggc accagcgtga
     2821 agatggatca gcacagaggc ttcctgcacg gccaggccaa gaatctgctg tgcggcttct
     2881 acggccggca cgcagagctg agatttctgg acctggtgcc tagcctgcag ctggatccag
     2941 cccagatcta tagggtgacc tggttcatca gctggtcccc atgcttttcc tggggatgtg
     3001 caggagaggt gcgcgccttc ctgcaggaga atacacacgt gcggctgaga atctttgccg
     3061 cccggatcta cgactatgat cctctgtaca aggaggccct gcagatgctg agagacgcag
     3121 gagcccaggt gtccatcatg acctatgatg agttcaagca ctgctgggac acatttgtgg
     3181 atcaccaggg ctgtcccttt cagccttggg acggactgga tgagcactcc caggccctgt
     3241 ctggcaggct gagggccatc ctgcagaacc agggcaatag cggaggatct agcggaggat
     3301 caagcggaag cgagactcct ggaaccagcg aaagcgcaac cccagaaagc agcggaggaa
     3361 gtagcggagg aagcatggca atgcagatgc agcttgaagc aaatgcagat acttcagtgg
     3421 aagaagaaag ctttggccca caacccattt cacggttaga gcagtgtggc ataaatgcca
     3481 acgatgtgaa gaaattggaa gaagctggat tccatactgt ggaggctgtt gcctatgcgc
     3541 caaagaagga gctaataaat attaagggaa ttagtgaagc caaagctgat aaaattctgg
     3601 ctgaggcagc taaattagtt ccaatgggtt tcaccactgc aactgaattc caccaaaggc
     3661 ggtcagagat catacagatt actactggct ccaaagagct tgacaaacta cttcaatctg
     3721 gcggatctag cggtggatct agcggctctg agacccctgg aacatccgaa tccgccactc
     3781 cagagagcag cggaggctct tctggaggat cagacaagaa gtacagcatc ggcctggcca
     3841 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     3901 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     3961 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     4021 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     4081 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     4141 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     4201 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     4261 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     4321 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     4381 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     4441 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
     4501 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
     4561 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
     4621 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     4681 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     4741 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
     4801 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     4861 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     4921 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     4981 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
     5041 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     5101 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     5161 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     5221 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
     5281 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     5341 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     5401 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     5461 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     5521 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     5581 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     5641 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     5701 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     5761 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     5821 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     5881 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     5941 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     6001 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     6061 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     6121 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     6181 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     6241 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     6301 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact
     6361 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     6421 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     6481 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     6541 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     6601 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     6661 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     6721 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     6781 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
     6841 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     6901 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     6961 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
     7021 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     7081 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     7141 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     7201 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     7261 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     7321 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     7381 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     7441 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
     7501 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     7561 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     7621 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     7681 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     7741 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     7801 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     7861 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc gacaaaaggc
     7921 cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatccgga gagggcagag
     7981 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaactaat ctgagcgaca
     8041 tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg atgctgcctg
     8101 aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg cacaccgcct
     8161 acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc gagtataagc
     8221 cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg ctgagcggag
     8281 gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag gagacaggca
     8341 agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa gaagtgatcg
     8401 gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt accgacgaaa
     8461 atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg gtcatccagg
     8521 attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg
     8581 gcagcgaatt cgagcccaag aagaagagga aagtcggaag cggagctact aacttcagcc
     8641 tgctgaagca ggctggagac gtggaggaga accctggacc tatggtgagc aagggcgagg
     8701 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     8761 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     8821 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     8881 atggagtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     8941 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     9001 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     9061 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     9121 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     9181 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     9241 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     9301 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     9361 ccgccgggat cactctcggc atggacgagc tgtacaagta ggttaacaat caacctctgg
     9421 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     9481 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     9541 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     9601 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     9661 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     9721 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     9781 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     9841 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     9901 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9961 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
    10021 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
    10081 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
    10141 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10201 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10261 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
    10321 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
    10381 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
    10441 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
    10501 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
    10561 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
    10621 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    10681 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10741 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    10801 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10861 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10921 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10981 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
    11041 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    11101 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    11161 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    11221 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    11281 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    11341 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    11401 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
    11461 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
    11521 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
    11581 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
    11641 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
    11701 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
    11761 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
    11821 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
    11881 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    11941 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    12001 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    12061 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    12121 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    12181 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    12241 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    12301 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    12361 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    12421 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    12481 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    12541 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    12601 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    12661 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    12721 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    12781 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    12841 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    12901 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    12961 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    13021 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    13081 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    13141 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    13201 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    13261 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    13321 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    13381 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    13441 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    13501 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    13561 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    13621 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    13681 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    13741 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    13801 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    13861 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    13921 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    13981 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    14041 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    14101 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    14161 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    14221 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    14281 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
    14341 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
    14401 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
    14461 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
    14521 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
    14581 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
    14641 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
    14701 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
    14761 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
    14821 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
    14881 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgg
//
