LOCUS       sequence-304828-        9106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of AID-BE4max-P2A-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Zhang X, Zhu B, Chen L, Xie L, Yu W, Wang Y, Li L, Yin S, Yang L, Hu
            H, Han H, Li Y, Wang L, Chen G, Ma X, Geng H, Huang W, Pang X, Yang 
            Z, Wu Y, Siwko S, Kurita R, Nakamura Y, Yang L, Liu M, Li D
  TITLE     Dual base editor catalyzes both cytosine and adenine base 
            conversions in human cells.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y.
  PUBMED    32483363
REFERENCE   2  (bases 1 to 9106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y."
COMMENT     Sequence Label: AID-BE4max-P2A-GFP
FEATURES             Location/Qualifiers
     source          1..9106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             417..437
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1029..1052,1053..1055,1056..5129)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1029..5129
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5160..5408
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5439..5687
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5730..5750
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5779..5796)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5785..6009
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6080..6096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6080..6096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6093..6115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6104..6120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6128..6134,6135..6152,6153..6158))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6173..6194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6311..6328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6482..7070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6562..6581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7241..8032,8033..8101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7864..7883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8102..8206)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8285..8304)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8496..8744
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8496..8516
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8667..8686
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8763..8838
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        join(8858..9106,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgccacc atgaagagga ccgccgatgg ctctgagttc gagagcccca
      421 agaagaagcg gaaggtggac agcctgctga tgaaccggag aaagttcctg tatcagttta
      481 agaatgtgag gtgggcaaag ggcaggcgcg agacctacct gtgctatgtg gtgaagcgga
      541 gagatagcgc cacatctttc agcctggact ttggctacct gcggaacaag aatggctgcc
      601 acgtggagct gctgtttctg agatacatct ccgactggga tctggaccca ggcaggtgtt
      661 atcgcgtgac ctggttcaca tcctggtctc cctgctacga ttgtgcccgg cacgtggccg
      721 actttctgag aggcaaccct aatctgtctc tgaggatctt caccgcccgc ctgtattttt
      781 gcgaggatag gaaggccgag ccagagggac tgaggcgcct gcacagggcc ggcgtgcaga
      841 tcgccatcat gacattcaag gactactttt attgttggaa caccttcgtg gagaatcacg
      901 agcggacatt taaggcctgg gagggactgc acgagaactc cgtgcggctg tctagacagc
      961 tgcggagaat cctgctgcca agcggctccg agaccccagg cacatctgag agcgccaccc
     1021 ctgagagcga caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg
     1081 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
     1141 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
     1201 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
     1261 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
     1321 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
     1381 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
     1441 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     1501 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     1561 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     1621 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     1681 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     1741 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     1801 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     1861 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     1921 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     1981 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     2041 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     2101 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     2161 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc
     2221 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     2281 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     2341 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     2401 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     2461 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     2521 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     2581 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     2641 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     2701 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     2761 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     2821 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     2881 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     2941 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     3001 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     3061 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     3121 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     3181 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     3241 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     3301 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     3361 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     3421 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     3481 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     3541 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     3601 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     3661 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     3721 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     3781 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     3841 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     3901 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     3961 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     4021 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     4081 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     4141 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
     4201 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
     4261 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     4321 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
     4381 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg
     4441 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
     4501 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
     4561 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc
     4621 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg
     4681 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg
     4741 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg
     4801 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga
     4861 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg
     4921 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg
     4981 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca
     5041 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac
     5101 ggatcgacct gtctcagctg ggaggcgaca gcggcgggag cggcgggagc ggggggagca
     5161 ctaatctgag cgacatcatt gagaaggaga ctgggaaaca gctggtcatt caggagtcca
     5221 tcctgatgct gcctgaggag gtggaggaag tgatcggcaa caagccagag tctgacatcc
     5281 tggtgcacac cgcctacgac gagtccacag atgagaatgt gatgctgctg acctctgacg
     5341 cccccgagta taagccttgg gccctggtca tccaggattc taacggcgag aataagatca
     5401 agatgctgag cggaggatcc ggaggatctg gaggcagcac caacctgtct gacatcatcg
     5461 agaaggagac aggcaagcag ctggtcatcc aggagagcat cctgatgctg cccgaagaag
     5521 tcgaagaagt gatcggaaac aagcctgaga gcgatatcct ggtccatacc gcctacgacg
     5581 agagtaccga cgaaaatgtg atgctgctga catccgacgc cccagagtat aagccctggg
     5641 ctctggtcat ccaggattcc aacggagaga acaaaatcaa aatgctgtct ggcggctcaa
     5701 aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc ggatcctaag
     5761 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5821 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5881 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5941 ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg gatgcggtgg
     6001 gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct
     6061 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga
     6181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8221 aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc
     8281 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     8341 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     8401 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     8461 ccagatatac gcgtggatcc ctgcagttaa ttaaagaggg cctatttccc atgattcctt
     8521 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     8581 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     8641 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     8701 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct tcgagaagac
     8761 ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     8821 gtggcaccga gtcggtgctt tttttatgca tggtaccgac attgattatt gactagttat
     8881 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     8941 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     9001 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     9061 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatc
//
