LOCUS       sequence-304829-       15760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ABE7.10-N-AIDmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15760)
  AUTHORS   Zhang X, Zhu B, Chen L, Xie L, Yu W, Wang Y, Li L, Yin S, Yang L, Hu
            H, Han H, Li Y, Wang L, Chen G, Ma X, Geng H, Huang W, Pang X, Yang 
            Z, Wu Y, Siwko S, Kurita R, Nakamura Y, Yang L, Liu M, Li D
  TITLE     Dual base editor catalyzes both cytosine and adenine base 
            conversions in human cells.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y.
  PUBMED    32483363
REFERENCE   2  (bases 1 to 15760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y."
COMMENT     Sequence Label: Lenti ABE7.10-N-AIDmax
FEATURES             Location/Qualifiers
     source          1..15760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2051..2262
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2328..2348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2904..2924
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4128..4151,4152..4154,4155..8228)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4128..8228
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8229..8276
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8292..8348
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8355..8357,8358..8360,8361..9071)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8355..8357,8358..8360,8361..9071)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8661..8680)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9008..9029
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        9107..9606
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9142..9165)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9539..9558
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9619..10218
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9619..9638)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10115..10135
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10234..10822
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10287..10307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10705..10716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10894..11127
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11153..11170)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11159..11383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11429..11857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11516..11535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11726..11747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11866..11886)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11871..12200
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12051..12186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12113..12132
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12248..12295
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12314..12688
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12818..12939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12855..12874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12909..12928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12988..13004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12988..13004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13001..13023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13012..13028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13036..13042,13043..13060,13061..13066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13081..13102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13219..13236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13390..13978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13470..13489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14149..14940,14941..15009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14772..14791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15010..15114)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15189..15208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        15380..15759
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg aattcgatat cgctagctag gtcttgaaag gagtgggaat tggctccggt
     2041 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     2101 ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     2161 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     2221 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggggcgcgc ctctagagcg
     2281 atcgcgccac catgaagagg accgccgatg gctctgagtt cgagagcccc aagaagaagc
     2341 ggaaggtgga cagcctgctg atgaaccgga gaaagttcct gtatcagttt aagaatgtga
     2401 ggtgggcaaa gggcaggcgc gagacctacc tgtgctatgt ggtgaagcgg agagatagcg
     2461 ccacatcttt cagcctggac tttggctacc tgcggaacaa gaatggctgc cacgtggagc
     2521 tgctgtttct gagatacatc tccgactggg atctggaccc aggcaggtgt tatcgcgtga
     2581 cctggttcac atcctggtct ccctgctacg attgtgcccg gcacgtggcc gactttctga
     2641 gaggcaaccc taatctgtct ctgaggatct tcaccgcccg cctgtatttt tgcgaggata
     2701 ggaaggccga gccagaggga ctgaggcgcc tgcacagggc cggcgtgcag atcgccatca
     2761 tgacattcaa ggactacttt tattgttgga acaccttcgt ggagaatcac gagcggacat
     2821 ttaaggcctg ggagggactg cacgagaact ccgtgcggct gtctagacag ctgcggagaa
     2881 tcctgctgcc aagcggcggc tcccccaaga agaagcggaa agtgggcagc tccggctcca
     2941 gcgaggtgga gttcagccac gagtactgga tgagacacgc cctgaccctg gctaagagag
     3001 cttgggatga gagagaggtg cccgtgggag ctgttctggt tcataacaac agggtgatcg
     3061 gcgagggatg gaacagacct atcgggagac acgacccaac cgctcatgct gaaatcatgg
     3121 ccctgagaca aggagggctg gtgatgcaaa attacagact gatcgacgca accctgtacg
     3181 tgaccctgga gccttgtgtg atgtgcgcag gagcaatgat ccactccaga atcggcagag
     3241 tggtgttcgg agctagagat gccaaaaccg gagccgctgg aagcctgatg gacgttctgc
     3301 atcaccccgg aatgaatcac agagtggaga taaccgaggg cattctggcc gacgagtgtg
     3361 ctgctctgct gtctgatttc ttcagaatga gaaggcagga aatcaaggcc cagaaaaagg
     3421 cccaaagcag caccgacagc ggaggatcta gcggaggatc aagcggaagc gagactcctg
     3481 gaaccagcga aagcgcaacc ccagaaagca gcggaggaag tagcggagga agctcagaag
     3541 tcgagttcag ccatgagtat tggatgagac atgctctgac cctggcaaag agagcaagag
     3601 acgagagaga ggtcccagtg ggagcagttc tggtgctgaa caacagagtg atcggggagg
     3661 ggtggaacag agcaatcgga ctgcacgacc ctacagcaca cgcagaaata atggcactga
     3721 gacaaggggg gctcgtgatg caaaactaca ggctgatcga cgccaccctg tacgtcacat
     3781 ttgagccctg tgtgatgtgt gccggagcca tgattcacag tagaatcggc cgggtggtgt
     3841 tcggtgtgag aaacgctaaa acaggcgccg ccggaagcct gatggatgtt ctgcattacc
     3901 ccggcatgaa tcaccgggtg gagatcacag agggcatcct ggctgacgaa tgtgccgctc
     3961 tgctgtgtta cttcttcaga atgccccgac aagtgttcaa cgcccagaag aaagcccagt
     4021 caagcaccga ctctggcgga tctagcggtg gatctagcgg ctctgagacc cctggaacat
     4081 ccgaatccgc cactccagag agcagcggag gctcttctgg aggatcagac aagaagtaca
     4141 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     4201 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     4261 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     4321 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     4381 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     4441 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     4501 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     4561 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     4621 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     4681 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     4741 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     4801 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     4861 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     4921 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     4981 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     5041 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     5101 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     5161 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     5221 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     5281 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     5341 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     5401 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     5461 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     5521 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     5581 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     5641 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     5701 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     5761 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     5821 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     5881 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     5941 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     6001 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     6061 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     6121 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     6181 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     6241 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     6301 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     6361 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     6421 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     6481 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     6541 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     6601 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     6661 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     6721 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     6781 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     6841 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     6901 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     6961 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     7021 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     7081 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     7141 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     7201 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     7261 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     7321 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     7381 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     7441 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     7501 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     7561 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     7621 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     7681 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     7741 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     7801 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     7861 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     7921 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     7981 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     8041 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     8101 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     8161 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     8221 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat
     8281 ccggcagcgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc
     8341 ctggaccgct cgagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     8401 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     8461 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     8521 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     8581 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     8641 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     8701 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     8761 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     8821 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     8881 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     8941 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     9001 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     9061 agctgtacaa gtactcagat ctggagctca agtgagctag cctaccgggt aggggaggcg
     9121 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     9181 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     9241 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     9301 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     9361 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     9421 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     9481 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     9541 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     9601 ctttcggaat tcgccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     9661 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     9721 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     9781 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     9841 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     9901 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     9961 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc gcccgaccac
    10021 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
    10081 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
    10141 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
    10201 cgcaagcccg gtgcctgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
    10261 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
    10321 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
    10381 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    10441 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    10501 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
    10561 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    10621 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
    10681 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    10741 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    10801 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
    10861 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
    10921 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10981 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    11041 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    11101 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
    11161 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    11221 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    11281 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    11341 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
    11401 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
    11461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    11521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    11581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    11641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    11701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    11761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    11821 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
    11881 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    11941 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    12001 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    12061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    12121 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
    12181 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
    12241 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
    12301 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
    12361 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
    12421 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
    12481 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
    12541 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
    12601 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
    12661 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
    12721 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    12781 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    12841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    12901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    12961 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    13021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    13081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    13141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    13201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    13261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    13321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    13381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    13441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    13501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    13561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    13621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    13681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    13741 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    13801 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    13861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    13921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    13981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    14041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    14101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    14161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    14221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    14281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    14341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    14401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    14461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    14521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    14581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    14641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    14701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    14761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    14821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    14881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    14941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    15001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    15061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    15121 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    15181 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    15241 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    15301 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
    15361 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
    15421 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
    15481 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
    15541 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
    15601 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
    15661 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
    15721 tggcagtaca tctacgtatt agtcatcgct attaccatgg
//
