LOCUS       sequence-304845-        7882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OE of DELE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Guo X, Aviles G, Liu Y, Tian R, Unger BA, Lin YT, Wiita AP, Xu K, 
            Correia MA, Kampmann M
  TITLE     Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI 
            pathway.
  JOURNAL   Nature. 2020 Mar;579(7799):427-432. doi: 10.1038/s41586-020-2078-2. 
            Epub 2020 Mar 4.
  PUBMED    32132707
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2078-2"; journalName: "Nature"; date: "2020-03";
            volume: "579"; issue: "7799"; pages: "427-432"
COMMENT     Sequence Label: pXG289
FEATURES             Location/Qualifiers
     source          1..7882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..804
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(806..839)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    846..871
                     /label=SA
                     /note="splice acceptor site"
     primer_bind     complement(894..911)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    900..1124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(1128..1161)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1221..1432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1433..2371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2328..2348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(3977..3979,3980..4690)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4019..4040)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4627..4648
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4783..4802)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4848..4903
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4902..4921)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(5252..5285)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5326..6162
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(6210..6798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6290..6309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6969..7760,7761..7829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7592..7611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgctttctct gaccagcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt
       61 ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt
      121 cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc
      181 cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg
      241 tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct
      301 gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct
      361 tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc
      421 gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc
      481 ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt
      541 catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc
      601 ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc
      661 ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct
      721 gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct
      781 gtcccctcca ccccacagtg gggcaataac ttcgtatagc atacattata cgaagttata
      841 agcttctgac ctcttctctt cctcccacag ggcctcgaaa acccgctgat cagcctcgac
      901 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      961 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1021 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1081 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgggtcata acttcgtata
     1141 gcatacatta tacgaagtta ttcgacattg attattgact agtggataac cgtattaccg
     1201 ccatgcatgt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     1261 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     1321 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     1381 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     1441 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     1501 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     1561 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     1621 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     1681 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     1741 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     1801 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     1861 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     1921 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     1981 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     2041 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     2101 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     2161 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     2221 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     2281 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     2341 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gctagcgaat tcagcgacat
     2401 gtggcgcctc ccgggactcc tgggccgagc tcttccccgt acactgggac ctagcctctg
     2461 gagggtgact cctaagtcca ccagcccaga tgggcctcag actacctcct ccactttgct
     2521 ggttcctgtg cctaacctcg acaggtcagg tccccatggc ccaggcacga gcgggggtcc
     2581 aaggtcccat ggatggaagg atgccttcca atggatgtct tcccgtgtct ccccgaacac
     2641 cctatgggat gccatatctt ggggcactct ggccgtgctg gccctgcagc tggcaaggca
     2701 gatccacttc caggcatccc tgccagcagg acctcagcgg gtagaacact gctcctggca
     2761 cagtcccctg gaccgtttcc tctcatctcc cttgtggcac ccatgctcct cactgcgaca
     2821 acacatcctc cccagccccg atggcccagc tcccaggcac actggcctca gggaacccag
     2881 gcttggccag gaagaagcct cagctcagcc ccggaacttc tcacacaatt ctttgagagg
     2941 agctcgtcct caggacccct ctgaggaagg tcccggtgat tttggcttcc tgcatgccag
     3001 tagtagcatc gagtccgagg caaaaccagc ccagcctcag cccactggtg aaaaggaaca
     3061 agataaatca aaaactcttt cccttgagga ggctgtgact tccattcagc agctcttcca
     3121 gctcagtgtt tccatcgctt tcaacttcct gggaacagag aacatgaaga gtggcgacca
     3181 cacggcagcc ttttcttact tccagaaagc tgcagcccgc ggctacagca aagcgcagta
     3241 caatgcgggc ttgtgtcatg agcatggcag aggcaccccc agggacatta gcaaggcggt
     3301 cctttattat cagttggctg ccagccaggg ccacagcctg gctcagtacc gctatgccag
     3361 gtgcctacta cgagacccag cctcttcgtg gaaccctgag cggcagaggg cagtgtcctt
     3421 gctgaagcag gctgcagact caggcttgag agaggcccaa gctttcctcg gggtgctttt
     3481 caccaaggag ccctacctgg atgagcagag agctgtgaaa tatctttggc ttgcagccaa
     3541 caatggggac tcacagagca ggtaccacct tggaatttgc tatgagaaag gccttggtgt
     3601 gcagaggaat ctgggagagg ccttgagatg ttaccagcag tcagccgctc tgggaaatga
     3661 ggccgcccag gagaggctgc gagccctctt ttccatgggg gctgcagccc cggggcccag
     3721 cgacctgaca gttacaggac tgaagtcttt ctccagcccc tccctctgca gcttgaacac
     3781 cctgctagca ggaacctcac gcctaccaca tgcctcgagc acaggcaacc ttggcctcct
     3841 ctgcagaagt gggcatctcg gagccagcct ggaagcctcc agcagggcta ttcccccaca
     3901 cccctaccca ctggaaagga gtgttgtaag actaggtttt ggcagcggcc gcgggatcca
     3961 ccggtcgcca accgcagtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4021 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
     4081 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4141 gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc
     4201 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4261 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
     4321 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4381 catcctgggg cacaagctgg agtacaactt caacagccac tacgtctata tcacggccga
     4441 caagcagaag aacagcatca aggctaactt caagatccgc cacaacgttg aggacggcag
     4501 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4561 gcccgacaac cactacctga gccatcagtc cgccctgagc aaagacccca acgagaagcg
     4621 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg attacacatg gcatggacga
     4681 gctgtacaag taaacgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     4741 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     4801 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     4861 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     4921 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     4981 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     5041 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     5101 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     5161 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     5221 atggagatcc ctcgacctgc agcccaagct tataacttcg tatagcatac attatacgaa
     5281 gttatgcggc cgcaccggta tgcatttaat taagatatca gctttactag ggacaggatt
     5341 ggtgacagaa aagccccatc cttaggcctc ctccttccta gtctcctgat attgggtcta
     5401 acccccacct cctgttaggc agattcctta tctggtgaca cacccccatt tcctggagcc
     5461 atctctctcc ttgccagaac ctctaaggtt tgcttacgat ggagccagag aggatcctgg
     5521 gagggagagc ttggcagggg gtgggaggga agggggggat gcgtgacctg cccggttctc
     5581 agtggccacc ctgcgctacc ctctcccaga acctgagctg ctctgacgcg gctgtctggt
     5641 gcgtttcact gatcctggtg ctgcagcttc cttacacttc ccaagaggag aagcagtttg
     5701 gaaaaacaaa atcagaataa gttggtcctg agttctaact ttggctcttc acctttctag
     5761 tccccaattt atattgttcc tccgtgcgtc agttttacct gtgagataag gccagtagcc
     5821 agccccgtcc tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa actccctttg
     5881 tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg cctctactcc ctttctcttt
     5941 ctccatcctt ctttccttaa agagtcccca gtgctatctg ggacatattc ctccgcccag
     6001 agcagggtcc cgcttcccta aggccctgct ctgggcttct gggtttgagt ccttggcaag
     6061 cccaggagag gcgctcaggc ttccctgtcc cccttcctcg tccaccatct catgcccctg
     6121 gctctcctgc cccttcccta caggggttcc tggctctgct ctaagggcga attcgccagg
     6181 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6241 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6301 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6361 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6421 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6481 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6541 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6601 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6661 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6721 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6781 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6841 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6901 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6961 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7021 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7081 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7141 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7201 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7261 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7321 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7381 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7441 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7501 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7561 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7621 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7681 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7741 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7801 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7861 tatcagggtt gaattcgccc tt
//
