LOCUS       sequence-304847-        8230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OE of HRI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Guo X, Aviles G, Liu Y, Tian R, Unger BA, Lin YT, Wiita AP, Xu K, 
            Correia MA, Kampmann M
  TITLE     Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI 
            pathway.
  JOURNAL   Nature. 2020 Mar;579(7799):427-432. doi: 10.1038/s41586-020-2078-2. 
            Epub 2020 Mar 4.
  PUBMED    32132707
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2078-2"; journalName: "Nature"; date: "2020-03";
            volume: "579"; issue: "7799"; pages: "427-432"
COMMENT     Sequence Label: pXG290
FEATURES             Location/Qualifiers
     source          1..8230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..804
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(806..839)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    846..871
                     /label=SA
                     /note="splice acceptor site"
     primer_bind     complement(894..911)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    900..1124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(1128..1161)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1221..1432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1433..2371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2328..2348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4325..4327,4328..5038)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4367..4388)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4975..4996
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5131..5150)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5196..5251
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5250..5269)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(5600..5633)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5674..6510
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(6558..7146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6638..6657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7317..8108,8109..8177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7940..7959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgctttctct gaccagcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt
       61 ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt
      121 cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc
      181 cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg
      241 tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct
      301 gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct
      361 tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc
      421 gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc
      481 ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt
      541 catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc
      601 ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc
      661 ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct
      721 gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct
      781 gtcccctcca ccccacagtg gggcaataac ttcgtatagc atacattata cgaagttata
      841 agcttctgac ctcttctctt cctcccacag ggcctcgaaa acccgctgat cagcctcgac
      901 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      961 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1021 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1081 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgggtcata acttcgtata
     1141 gcatacatta tacgaagtta ttcgacattg attattgact agtggataac cgtattaccg
     1201 ccatgcatgt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     1261 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     1321 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     1381 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     1441 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     1501 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     1561 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     1621 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     1681 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     1741 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     1801 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     1861 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     1921 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     1981 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     2041 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     2101 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     2161 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     2221 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     2281 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     2341 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gctagcggcc gcgaattcgc
     2401 caccatgcag gggggcaact ccggggtccg caagcgcgaa gaggagggcg acggggctgg
     2461 ggctgtggct gcgccgccag ctatagattt tcccgcggaa ggtccagacc ccgaatacga
     2521 cgagtccgac gttccagcag aaatccaggt gttaaaagaa cccctacaac agccaacctt
     2581 cccttttgca gttgcaaacc aactcttgct ggtttctttg ctggagcact tgagccacgt
     2641 gcatgaacca aacccacttc gttcaagaca ggtgtttaag ctactttgcc agacgtttat
     2701 caaaatgggg ctgctgtctt ctttcacttg tagtgacgag tttagctcat tgagactaca
     2761 tcacaacaga gctattactc acttaatgag gtctgctaaa gagagagttc gtcaggatcc
     2821 ttgtgaggat atttctcgta tccagaaaat cagatcaagg gaagtagcct tggaagcaca
     2881 aacttcacgt tacttaaatg aatttgaaga acttgccatc ttaggaaaag gtggatacgg
     2941 aagagtatac aaggtcagga ataaattaga tggtcagtat tatgcaataa aaaaaatcct
     3001 gattaagggt gcaactaaaa cagtttgcat gaaggtccta cgggaagtga aggtgctggc
     3061 aggtcttcag caccccaata ttgttggcta tcacaccgcg tggatagaac atgttcatgt
     3121 gattcagcca cgagcagaca gagctgccat tgagttgcca tctctggaag tgctctccga
     3181 ccaggaagag gacagagagc aatgtggtgt taaaaatgat gaaagtagca gctcatccat
     3241 tatctttgct gagcccaccc cagaaaaaga aaaacgcttt ggagaatctg acactgaaaa
     3301 tcagaataac aagtcggtga agtacaccac caatttagtc ataagagaat ctggtgaact
     3361 tgagtcgacc ctggagctcc aggaaaatgg cttggctggt ttgtctgcca gttcaattgt
     3421 ggaacagcag ctgccactca ggcgtaattc ccacctagag gagagtttca catccaccga
     3481 agaatcttcc gaagaaaatg tcaacttttt gggtcagaca gaggcacagt accacctgat
     3541 gctgcacatc cagatgcagc tgtgtgagct ctcgctgtgg gattggatag tcgagagaaa
     3601 caagcggggc cgggagtatg tggacgagtc tgcctgtcct tatgttatgg ccaatgttgc
     3661 aacaaaaatt tttcaagaat tggtagaagg tgtgttttac atacataaca tgggaattgt
     3721 gcaccgagat ctgaagccaa gaaatatttt tcttcatggc cctgatcagc aagtaaaaat
     3781 aggagacttt ggtctggcct gcacagacat cctacagaag aacacagact ggaccaacag
     3841 aaacgggaag agaacaccaa cacatacgtc cagagtgggt acttgtctgt acgcttcacc
     3901 cgaacagttg gaaggatctg agtatgatgc caagtcagat atgtacagct tgggtgtggt
     3961 cctgctagag ctctttcagc cgtttggaac agaaatggag cgagcagaag ttctaacagg
     4021 tttaagaact ggtcagttgc cggaatccct ccgtaaaagg tgtccagtgc aagccaagta
     4081 tatccagcac ttaacgagaa ggaactcatc gcagagacca tctgccattc agctgctgca
     4141 gagtgaactt ttccaaaatt ctggaaatgt taacctcacc ctacagatga agataataga
     4201 gcaagaaaaa gaaattgcag aactaaagaa gcagctaaac ctcctttctc aagacaaagg
     4261 ggtgagggat gacggaaagg atgggggcgt gggatctaga gggatccacc ggtcgccaac
     4321 cgcagtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4381 cggcgacgta aacggccaca agttcagcgt ccgcggcgag ggcgagggcg atgccaccaa
     4441 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     4501 cctcgtgacc accttcggct acggcgtggc ctgcttcagc cgctaccccg accacatgaa
     4561 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctc
     4621 tttcaaggac gacggtacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4681 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4741 caagctggag tacaacttca acagccacta cgtctatatc acggccgaca agcagaagaa
     4801 cagcatcaag gctaacttca agatccgcca caacgttgag gacggcagcg tgcagctcgc
     4861 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4921 ctacctgagc catcagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     4981 cctgctggag ttcgtgaccg ccgccgggat tacacatggc atggacgagc tgtacaagta
     5041 aacgcgtgaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     5101 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     5161 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     5221 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     5281 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     5341 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     5401 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     5461 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     5521 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     5581 cgacctgcag cccaagctta taacttcgta tagcatacat tatacgaagt tatgcggccg
     5641 caccggtatg catttaatta agatatcagc tttactaggg acaggattgg tgacagaaaa
     5701 gccccatcct taggcctcct ccttcctagt ctcctgatat tgggtctaac ccccacctcc
     5761 tgttaggcag attccttatc tggtgacaca cccccatttc ctggagccat ctctctcctt
     5821 gccagaacct ctaaggtttg cttacgatgg agccagagag gatcctggga gggagagctt
     5881 ggcagggggt gggagggaag ggggggatgc gtgacctgcc cggttctcag tggccaccct
     5941 gcgctaccct ctcccagaac ctgagctgct ctgacgcggc tgtctggtgc gtttcactga
     6001 tcctggtgct gcagcttcct tacacttccc aagaggagaa gcagtttgga aaaacaaaat
     6061 cagaataagt tggtcctgag ttctaacttt ggctcttcac ctttctagtc cccaatttat
     6121 attgttcctc cgtgcgtcag ttttacctgt gagataaggc cagtagccag ccccgtcctg
     6181 gcagggctgt ggtgaggagg ggggtgtccg tgtggaaaac tccctttgtg agaatggtgc
     6241 gtcctaggtg ttcaccaggt cgtggccgcc tctactccct ttctctttct ccatccttct
     6301 ttccttaaag agtccccagt gctatctggg acatattcct ccgcccagag cagggtcccg
     6361 cttccctaag gccctgctct gggcttctgg gtttgagtcc ttggcaagcc caggagaggc
     6421 gctcaggctt ccctgtcccc cttcctcgtc caccatctca tgcccctggc tctcctgccc
     6481 cttccctaca ggggttcctg gctctgctct aagggcgaat tcgccaggaa ccgtaaaaag
     6541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttga
     8221 attcgccctt
//
