LOCUS       sequence-304853-       10883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HA-tagged (C terminal) human RPN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Liang JR, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, Olzmann 
            JA, Corn JE
  TITLE     A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial 
            Metabolism and ER-Resident UFMylation.
  JOURNAL   Cell. 2020 Mar 19;180(6):1160-1177.e20. doi: 
            10.1016/j.cell.2020.02.017. Epub 2020 Mar 10.
  PUBMED    32160526
REFERENCE   2  (bases 1 to 10883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.02.017"; journalName: "Cell"; date: 
            "2020-03-19- 19"; volume: "180"; issue: "6"; pages: "1160-1177.e20"
COMMENT     Sequence Label: plenti-X1-Neo-RPN1-HA
FEATURES             Location/Qualifiers
     source          1..10883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4170..4194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4325..5503
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4556..5494
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5451..5471
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5520..5538
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             6493..6501
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7431..7457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(7466..7490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7521..8109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7574..7594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7992..8003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8160..8659
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8195..8218)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8592..8611
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8673..9476
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8736..8755)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9346..9365
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9620..9853
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9925..10046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9962..9981)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10016..10035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10086..10221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10242..10260)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10270..10287)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10270..10286)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10279..10301)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10428..10856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10515..10534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10725..10746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctccacca
     4201 tgggaaccga cattgattat tgactaggct tttgcaaaaa gctttgcaaa gatggataaa
     4261 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     4321 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4381 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     4441 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4501 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     4561 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     4621 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     4681 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     4741 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     4801 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     4861 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     4921 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     4981 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5041 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5101 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     5161 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5221 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5281 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5341 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5401 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5461 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact
     5521 aatacgactc actataggga gacccaagct ggctaggtaa gcttggtacc gagctcggat
     5581 ccatggaggc gccagccgcc ggcttgtttc tgctcctgtt gcttgggact tgggccccgg
     5641 cgccgggcag cgcctcctcc gaggcaccgc cgctgatcaa tgaggacgtg aagcgcacag
     5701 tggacctaag cagccacctg gctaaggtga cggccgaggt ggtcctggcg cacctgggcg
     5761 gcggctccac gtcccgagct acctctttcc tgctggcttt ggagcctgag ctcgaggccc
     5821 ggctggcgca cctgggcgtg caggtaaagg gagaagatga ggaagagaac aatttggaag
     5881 tacgtgaaac caaaattaag ggtaaaagtg ggagattctt cacagtcaag ctcccagttg
     5941 ctcttgatcc tggggccaag atttcagtca ttgtggaaac agtctacacc catgtgcttc
     6001 atccatatcc aacccagatc acccagtcag agaaacagtt tgtggtgttt gaggggaacc
     6061 attatttcta ctctccctat ccaacgaaga cacaaaccat gcgtgtgaag cttgcctctc
     6121 gaaatgtgga gagctacacc aagctgggga accccacgcg ctctgaggac ctactggatt
     6181 atgggccttt cagagatgtg cctgcctata gtcaggatac ttttaaagta cattatgaga
     6241 acaacagccc tttcctgacc atcaccagca tgacccgagt cattgaagtc tctcactggg
     6301 gtaatattgc tgtggaagaa aatgtggact taaagcacac aggagctgtg cttaaggggc
     6361 ctttctcacg ctatgattac cagagacagc cagatagtgg aatatcctcc atccgttctt
     6421 ttaagaccat ccttcctgct gctgcccagg atgtttatta ccgggatgag attggcaatg
     6481 tttctaccag ccacctcctt attttggatg actctgtaga gatggaaatc cggcctcgct
     6541 tccctctctt tggcgggtgg aagacccatt acatcgttgg ctacaacctc ccaagctatg
     6601 agtacctcta taatttgggt gaccagtatg cactgaagat gaggtttgtg gaccatgtgt
     6661 ttgatgaaca agtgatagat tctctgactg tgaagatcat cctgcctgaa ggagccaaga
     6721 acattgaaat tgatagtccc tatgaaatca gccgtgcccc agatgagctg cactacacct
     6781 atctggatac atttggccgc cctgtgattg ttgcctacaa gaaaaatctg gtagaacagc
     6841 acattcagga cattgtggtc cactacacgt tcaacaaggt gctcatgctg caggagcccc
     6901 tgctggtggt ggcggccttc tacatcctgt tcttcaccgt tatcatctat gttcggctgg
     6961 acttctccat caccaaggat ccagccgcag aagccaggat gaaggtagcc tgcatcacag
     7021 agcaggtctt gaccctggtc aacaagagaa taggccttta ccgtcacttt gacgagaccg
     7081 tcaataggta caagcaatcc cgggacatct ccaccctcaa cagtggcaag aagagcctgg
     7141 agactgaaca caaggccttg accagtgaga ttgcactgct gcagtccagg ctgaagacag
     7201 agggctctga tctgtgcgac agagtgagcg aaatgcagaa gctggatgca caggtcaagg
     7261 agctggtgct gaagtcggcg gtggaggctg agcgcctggt ggctggcaag ctcaagaaag
     7321 acacgtacat tgagaatgag aagctcatct caggaaagcg ccaggagctg gtcaccaaga
     7381 tcgaccacat cctggatgcc ctgggtggat ctggaggttc tggtggaatg tacccatacg
     7441 atgttccaga ttacgcttga tctagaccca gctttcttgt acaaagtggt tgatatccag
     7501 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     7561 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7621 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7681 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7741 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7801 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7861 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7921 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7981 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8041 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8101 cctccccgcc tggacctgca ggattctgca gatatccgga ctagtgatct aattctaccg
     8161 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     8221 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     8281 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     8341 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     8401 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     8461 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     8521 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     8581 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     8641 ctcatctccg ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat
     8701 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     8761 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     8821 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     8881 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     8941 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     9001 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     9061 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     9121 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     9181 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     9241 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     9301 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     9361 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     9421 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaacgc
     9481 gtcgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc
     9541 gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9601 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt
     9661 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9721 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9781 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9841 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     9901 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     9961 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10021 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
    10081 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    10141 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    10201 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
    10261 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
    10321 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
    10381 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
    10441 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    10501 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    10561 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    10621 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    10681 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10741 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    10801 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
    10861 caaaatatta acgcttacaa ttt
//
