LOCUS       sequence-304862-        5820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc 
            Transferase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pENTR1A FKBP12F36V-OGT
FEATURES             Location/Qualifiers
     source          1..5820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(488..490,491..811)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             818..844
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             818..844
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             848..874
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    890..914
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4049..4148)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4260..4279)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4271..5080
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4338..4357)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5170..5758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5659..5678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccatg ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa
      541 gcgcggccag acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaagttga
      601 ttcctcccgg gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg
      661 aggctgggaa gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc
      721 tccagattat gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct
      781 cgtcttcgat gtggagcttc taaaactgga aggcggctac ccctacgacg tgcccgacta
      841 cgccggctat ccgtatgatg tcccggacta tgcaggcatc gatagatcaa caagtttgta
      901 caaaaaagca ggctttgcgt cttccgtggg caacgtggcc gacagcacag ggttagctga
      961 gttggcacat cgagaatatc aggcaggaga ttttgaggca gctgagagac actgcatgca
     1021 gctctggaga caagagccag acaatactgg tgtgctttta ttactttcat ctatacactt
     1081 ccagtgtcga aggctggaca gatctgctca ctttagcact ctggcaatta aacagaaccc
     1141 ccttctggca gaagcttatt cgaatttggg gaatgtgtac aaggaaagag ggcagttgca
     1201 ggaggcaatt gagcattatc gacatgcatt gcgtctcaaa cctgatttca tcgatggtta
     1261 tattaacctg gcagccgcct tggtagcagc gggtgacatg gaaggggcag tacaagctta
     1321 cgtctctgct cttcagtaca atcctgattt gtactgtgtt cgcagtgacc tggggaacct
     1381 gctcaaagcc ctgggtcgct tggaagaagc caaggcatgt tatttgaaag caattgagac
     1441 gcaaccgaac tttgcagtag cttggagtaa tcttggctgt gttttcaatg cacaagggga
     1501 aatttggctt gcaattcatc actttgaaaa ggctgtcacc cttgacccaa actttctgga
     1561 tgcttatatc aatttaggaa atgtcttgaa agaggcacgc atttttgaca gagctgtggc
     1621 agcttatctt cgtgccctaa gtttgagtcc aaatcacgca gtggtgcacg gcaacctggc
     1681 ttgtgtatac tatgagcaag gcctgataga tctggcaata gacacctaca ggcgggctat
     1741 cgaactacaa ccacatttcc ctgatgctta ctgcaaccta gccaatgctc tcaaagagaa
     1801 gggcagtgtt gctgaagcag aagattgtta taatacagct ctccgtctgt gtcccaccca
     1861 tgcagactct ctgaataacc tagccaatat caaacgagaa cagggaaaca ttgaagaggc
     1921 agttcgcttg tatcgtaaag cattagaagt cttcccagag tttgctgctg cccattcaaa
     1981 tttagcaagt gtactgcagc agcagggaaa actgcaggaa gctctgatgc attataagga
     2041 ggctattcga atcagtccta cctttgctga tgcctactct aatatgggaa acactctaaa
     2101 ggagatgcag gatgttcagg gagccttgca gtgttatacg cgtgccatcc aaattaatcc
     2161 tgcatttgca gatgcacata gcaatctggc ttccattcat aaggattcag ggaatattcc
     2221 agaagccata gcttcttacc gcacggctct gaaacttaag cctgattttc ctgatgctta
     2281 ttgtaacttg gctcattgcc tgcagattgt ctgtgattgg acagactatg atgagcgaat
     2341 gaagaagttg gtcagtattg tggctgacca gttagagaag aataggttgc cttctgtgca
     2401 tcctcatcat agtatgctat atcctctttc tcatggcttc aggaaggcta ttgctgagag
     2461 gcacggcaac ctgtgcttag ataagattaa tgttcttcat aaaccaccat atgaacatcc
     2521 aaaagacttg aagctcagtg atggtcggct gcgtgtagga tatgtgagtt ccgactttgg
     2581 gaatcatcct acttctcacc ttatgcagtc tattccaggc atgcacaatc ctgataaatt
     2641 tgaggtgttc tgttatgccc tgagcccaga cgatggcaca aacttccgag tgaaggtgat
     2701 ggcagaagcc aatcatttca ttgatctttc tcagattcca tgcaatggaa aagcagctga
     2761 tcgcatccat caggatggaa ttcatatcct tgtaaatatg aatggctata ctaagggcgc
     2821 tcgaaatgag ctttttgctc tcaggccagc tcctattcag gcaatgtggc tgggataccc
     2881 tgggacgagt ggtgcgcttt tcatggatta tattatcact gatcaggaaa cttcgccagc
     2941 tgaagttgct gagcagtatt ccgagaaatt ggcttatatg ccccacactt tttttattgg
     3001 tgatcatgct aatatgttcc ctcacctgaa gaaaaaagca gtcatcgatt ttaagtccaa
     3061 tgggcacatt tatgacaatc ggatagttct gaatggcatc gacctcaaag catttcttga
     3121 tagtctacca gatgtgaaaa ttgtcaagat gaagtgtcct gatggaggag acaatgcaga
     3181 tagcagtaac acagctctta atatgcctgt tattcctatg aatactattg cagaagcagt
     3241 tattgaaatg attaaccgag gacagattca aataacaatt aatggattca gtattagcaa
     3301 tggactggca actactcaga tcaacaataa ggctgcaact ggagaggagg ttccccgtac
     3361 cattattgta accacccgtt ctcagtacgg gttaccagaa gatgccatcg tatactgtaa
     3421 ctttaatcag ttgtataaaa ttgacccttc tactttgcag atgtgggcaa acattctgaa
     3481 gcgtgttccc aatagtgtac tctggctgtt gcgttttcca gcagtaggag aacctaatat
     3541 tcaacagtat gcacaaaaca tgggcctgcc ccagaaccgt atcatttttt cacctgttgc
     3601 tcctaaagag gaacacgtca ggagaggcca gctggctgat gtctgcttgg acactccact
     3661 ctgtaatggg cacaccacag ggatggatgt cctctgggca gggaccccca tggtgactat
     3721 gccaggagag actcttgctt ctcgagttgc agcatcccag ctcacttgct taggttgtct
     3781 tgagcttatt gctaaaaaca gacaagaata tgaagacata gctgtgaagc tgggaactga
     3841 tctagaatac ctgaagaaag ttcgtggcaa agtctggaag caaagaatat ctagccctct
     3901 gttcaacacc aaacaataca caatggaact agagcggctc tatctacaga tgtgggagca
     3961 ttatgcagct ggcaacaaac ctgaccacat gattaagcct gttgaagtca ctgagtcagc
     4021 ataacgcggc cgcactcgag atatctagac ccagctttct tgtacaaagt tggcattata
     4081 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     4141 attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     4201 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     4261 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     4321 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     4381 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     4441 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     4501 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     4561 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     4621 tattgttgat gcgctggcag tgtccctgcg ccggttgcat tcgattcctg tttgtaattg
     4681 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     4741 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     4801 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     4861 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     4921 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     4981 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     5041 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     5101 gtaacattat tcagattggg ccccgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5281 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
