LOCUS       sequence-304873-        3177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV2 TEV-ORF7A
FEATURES             Location/Qualifiers
     source          1..3177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(32..59)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(151..237)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     286..308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     300..317
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     301..317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    333..432
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             432..452
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(820..919)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(936..955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(937..955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(960..976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(960..976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1407..2198
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2291..2879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2780..2799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3033..3050
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
       61 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
      121 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
      181 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
      241 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
      301 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
      361 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      421 aaaaagttgg cgaaaacctg tacttccaag gcatgaagat catcctgttc ctggccctga
      481 tcaccctggc tacctgcgag ctgtaccact accaggaatg cgtgcgcggt accaccgtgc
      541 tgctgaagga gccttgcagc tccggcacct acgaaggaaa ctcccccttc caccctctgg
      601 ccgacaacaa gttcgctctg acctgcttct ctactcagtt cgccttcgct tgccctgatg
      661 gtgtgaagca cgtgtaccag ctgcgcgcca ggtctgtgag cccaaagctg ttcatcagac
      721 aggaggaagt gcaggagctg tacagcccca tcttcctgat cgtggccgct atcgtgttca
      781 tcaccctgtg cttcaccctg aagcgtaaga ccgaataata cccaactttc ttgtacaaag
      841 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
      901 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
      961 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1021 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     1081 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     1141 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     1201 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     1261 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     1321 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     1381 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     1441 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     1501 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     1561 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     1621 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     1681 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     1741 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     1801 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     1861 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     1921 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     1981 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2041 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     2101 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     2161 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     2221 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     2281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2401 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3121 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgta
//
