LOCUS       sequence-304874-        8798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, 
            Nakamura M, Sasaki E, Shiozawa S, Okano H
  TITLE     A versatile toolbox for knock-in gene targeting based on the 
            Multisite Gateway technology.
  JOURNAL   PLoS One. 2019 Aug 27;14(8):e0221164. doi: 
            10.1371/journal.pone.0221164. eCollection 2019.
  PUBMED    31454364
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-27"; volume: "14(8):e0221164. doi"; pages: " 
            10.1371/journal.pone.0221164. eCollection 2019"
COMMENT     Sequence Label: pUC-DEST-hPROX1-tdTomato-PuroTK
FEATURES             Location/Qualifiers
     source          1..8798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..23
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15..32
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16..32
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             815..871
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             872..2296
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
     repeat_region   2306..2368
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    2539..2563
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2570..3069
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2605..2628)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3002..3021
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3093..3689
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3093..3112)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3589..3609
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3696..4691
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    complement(5057..5081)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   5355..5389
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(6133..6153)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6214..6230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6214..6230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6227..6249)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6238..6254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6262..6268,6269..6286,6287..6292))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6307..6328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6445..6462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6616..7204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6696..6715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7375..8166,8167..8235))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7998..8017
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8236..8340)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8408..8426
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8464..8486)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8586..8605
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcggga
       61 tatctgaatt atcaactttg tataataaag ttgttgccac catgggcgca tctgagtcaa
      121 caatcctctg agacaaacag ataactgaga ttttagaaga ttttcttcat ttaaagcttg
      181 ggtttaattt ataaagaagc ccaactattt gttattctat tttgagaacg tattttgttt
      241 tcatcatggc aatcaaaaag aaataggatt caaattctga aaaaataatt ggagactttc
      301 ttctggatag cacttattta ataaagtgag gaatcccaaa agtcacatcc catattccta
      361 tcctaatatc cacaatgaaa tcccagtttt tcaataggtc tgcgttggat ctttcataca
      421 ctcttcttaa aacaaagctg tcaaccccac atcacaatgc ttctatatat aatgacttta
      481 cattaaaaga atagaagcca gctattttta gaaaatgcag gtgccatgta agcccctttc
      541 tgcaagaatg atcttagctc agtttccttg gaataactgt agacttgaaa ctgaaaactt
      601 tattaatgcc attgtctcct tgtatcagca ggttccagag agattcctgg aagttgctca
      661 gatcacatta cgggagtttt tcaatgccat tatcgcaggc aaagatgttg atccttcctg
      721 gaagaaggcc atatacaagg tcatctgcaa gctggatagt gaagtccctg agattttcaa
      781 atccccgaac tgcctacaag agctgcttca tgaggccacg aacttctctc tgttaaagca
      841 agcaggagac gtggaagaaa accccggtcc tgttagcaag ggcgaggagg tcatcaaaga
      901 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
      961 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1021 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     1081 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     1141 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     1201 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     1261 ccccgacggc cccgtaatgc agaaaaagac catgggctgg gaggcctcca ccgagcgcct
     1321 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     1381 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     1441 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     1501 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
     1561 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
     1621 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     1681 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     1741 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     1801 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     1861 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     1921 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     1981 cttccccccc gacggccccg taatgcagaa aaagaccatg ggctgggagg cctccaccga
     2041 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     2101 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     2161 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     2221 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     2281 ggacgagctg tacaaggaat tttaacccta gaaagataat catattgtga cgtacgttaa
     2341 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtcccgtg
     2401 aggcgtttga atagatatta agttttatta tatttacact tacatactaa taataaattc
     2461 aacaaacaat tcttttacgt gacttttaag atttaactca aacaaaaact caaaatttct
     2521 tctataaagt aacaaaactc aagtttgtac aaaaaagcag aattctaccg ggtaggggag
     2581 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     2641 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     2701 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     2761 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     2821 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     2881 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     2941 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     3001 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     3061 ggcctttcga ccgatcatca agcttgatcc tcatgaccga gtacaagccc acggtgcgcc
     3121 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     3181 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     3241 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     3301 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     3361 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     3421 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     3481 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     3541 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     3601 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     3661 cctggtgcat gacccgcaag cccggtgccg gatccatgcc cacgctactg cgggtttata
     3721 tagacggtcc ccacgggatg gggaaaacca ccaccacgca actgctggtg gccctgggtt
     3781 cgcgcgacga tatcgtctac gtacccgagc cgatgactta ctggcgggtg ctgggggctt
     3841 ccgagacaat cgcgaacatc tacaccacac aacaccgcct cgaccagggt gagatatcgg
     3901 ccggggacgc ggcggtggta atgacaagcg cccagataac aatgggcatg ccttatgccg
     3961 tgaccgacgc cgttctggct cctcatatcg ggggggaggc tgggagctca catgccccgc
     4021 ccccggccct caccctcatc ttcgaccgcc atcccatcgc cgccctcctg tgctacccgg
     4081 ccgcgcggta ccttatgggc agcatgaccc cccaggccgt gctggcgttc gtggccctca
     4141 tcccgccgac cttgcccggc accaacatcg tgcttggggc ccttccggag gacagacaca
     4201 tcgaccgcct ggccaaacgc cagcgccccg gcgagcggct ggacctggct atgctggctg
     4261 cgattcgccg cgtttacggg ctacttgcca atacggtgcg gtatctgcag tgcggcgggt
     4321 cgtggcggga ggactgggga cagctttcgg ggacggccgt gccgccccag ggtgccgagc
     4381 cccagagcaa cgcgggccca cgaccccata tcggggacac gttatttacc ctgtttcggg
     4441 cccccgagtt gctggccccc aacggcgacc tgtataacgt gtttgcctgg gccttggacg
     4501 tcttggccaa acgcctccgt tccatgcacg tctttatcct ggattacgac caatcgcccg
     4561 ccggctgccg ggacgccctg ctgcaactta cctccgggat ggtccagacc cacgtcacca
     4621 cccccggctc cataccgacg atatgcgacc tggcgcgcac gtttgcccgg gagatggggg
     4681 aggctaactg agctctagct tatcgactcg ccgatagtgg aaaccgacgc cccagcactc
     4741 gtccgagggc aaaggaatag tcgagaaatt gatgatctat taaacaataa agatgtccac
     4801 atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac ctacattttg
     4861 aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa tgcctgctct
     4921 ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat atcataattt
     4981 aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc tatagatcct
     5041 ctagagtcga gggctgcagc agctttcttg tacaaagtgg gatatctata acaagaaaat
     5101 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     5161 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     5221 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     5281 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     5341 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taatagaaat
     5401 ttcaacaact ctttttgaat gtatgaagag tagcagtccc ctttaaatgt ccaagttata
     5461 tgtgtctaga ttttgatttc atatatatgt gtatgggagg catggatatg ttatgaaatc
     5521 agctggtaat tcctcctcat cacgtttctc tcattttctt ttgttttcca ttgcaagggg
     5581 atggttgttt tctttctgcc tttagtttgc ttttgcccaa ggcccttaac atttggacac
     5641 ttaaaatagg gttaattttc agggaaaaag aatgttggcg tgtgtaaagt ctctattagc
     5701 aatgaaggga atttgttaac gatgcatcca cttgattgat gacttattgc aaatggcggt
     5761 tggctgagga aaacccatga cacagcacaa ctctacagac agtgatgtgt ctcttgtttc
     5821 tactgctaag aaggtctgaa aatttaatga aaccacttca tacatttaag tattttgttt
     5881 ggtttgaact caatcagtag cttttcctta catgtttaaa aataattcca atgacagatg
     5941 agcagctcac ttttccaaag taccccaaaa ggccaaatta aaaaagaaaa ataatcactc
     6001 tcaagccttg tctaagaaaa gaggcaaact ctgaaagtcg taccagtttc ttctggaggc
     6061 aaagcaattt tgcacaaaac cagctctctc aagatgagac tagaaattca tacctggtct
     6121 tgtagccacc aacaactttt ctatacaaag ttgatagctt gatatcaata ctctagagga
     6181 tccccgggta ccgagctcga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6241 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6301 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6361 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7501 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7981 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8041 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8101 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8161 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8221 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8281 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8341 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8401 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     8461 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     8521 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     8581 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     8641 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     8701 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     8761 tgctgcaagg cgattaagtt gggtaacgcc agggtttt
//
