LOCUS       sequence-304892-        5218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMP71 expression vector containing Kaede.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Bresser K, Dijkgraaf FE, Pritchard CEJ, Huijbers IJ, Song JY, Rohr 
            JC, Scheeren FA, Schumacher TN
  TITLE     A mouse model that is immunologically tolerant to reporter and 
            modifier proteins.
  JOURNAL   Commun Biol. 2020 May 29;3(1):273. doi: 10.1038/s42003-020-0979-0.
  PUBMED    32472011
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-29"; volume: "3(1):273. doi"; pages: " 
            10.1038/s42003-020-0979-0"
COMMENT     Sequence Label: pMP71-Kaede
FEATURES             Location/Qualifiers
     source          1..5218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1089..1766
                     /codon_start=1
                     /product="Kaede green-to-red photoconvertible fluorescent 
                     protein (Ando et al., 2002)"
                     /label=Kaede
                     /translation="MSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVVK
                     EGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIAT
                     NDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALLL
                     KGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLPD
                     NVK"
     LTR             1959..2549
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2574..2590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2574..2590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2587..2609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2598..2614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2622..2628,2629..2646,2647..2652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2667..2688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2805..2822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2976..3564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3056..3075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3735..4526,4527..4595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4358..4377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4596..4700)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4768..4786
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4824..4846)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4946..4965
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5159..5181
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5173..5190
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5174..5190
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga
       61 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
      121 gctcagggcc aagaacagtt ggaacagcag aatatgggcc aaacaggata tctgtggtaa
      181 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc
      241 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
      301 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
      361 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact
      421 gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg
      481 actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc gggggtcttt
      541 catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga cccccccgcc
      601 gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc gtgtttgtgc
      661 cggcatctaa tgtttgcgcc tgcgtctgta ctagttggct aactagatct gtatctggcg
      721 gtcccgcgga agaactgacg agttcgtatt cccggccgca gcccctggga gacgtcccag
      781 cggcctcggg ggcccgtttt gtggcccatt ctgtatcagt taacctaccc gagtcggact
      841 ttttggagct ccgccactgt ccgaggggta cgtggctttg ttgggggacg agagacagag
      901 acacttcccg cccccgtctg aatttttgct ttcggtttta cgccgaaacc gcgccgcgcg
      961 tcttgtctgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1021 tgaaaattag ctccacaaag ttaagtaata gtccctctct ccaagctcac ttacaggcgg
     1081 ccgccaccat gagtctgatt aaaccagaaa tgaagatcaa gctgcttatg gaaggcaatg
     1141 taaacgggca ccagtttgtt attgagggag atggaaaagg ccatcctttt gagggaaaac
     1201 agagtatgga ccttgtagtc aaagaaggcg cacctctccc ttttgcctac gatatcttga
     1261 caacagcatt ccattatggt aacagggttt ttgctaaata cccagaccat ataccagact
     1321 acttcaagca gtcgtttccc aaagggtttt cttgggagcg aagcctgatg ttcgaggacg
     1381 ggggcgtttg catcgctaca aatgacataa cactgaaagg agacactttt tttaacaaag
     1441 ttcgatttga tggcgtaaac tttcccccaa atggtcctgt tatgcagaag aagactctga
     1501 aatgggaggc atccactgag aaaatgtatt tgcgtgatgg agtgttgacg ggcgatatta
     1561 caatggctct gctgcttaaa ggagatgtcc attaccgatg tgacttcaga actacttaca
     1621 aatctaggca ggagggtgtc aagttgccag gatatcactt tgtcgatcac tgcatcagca
     1681 tattgaggca tgacaaagac tacaacgagg ttaagctgta tgagcacgct gttgcccatt
     1741 ctggattgcc ggacaacgtc aagtaaggat ccaagcttag gcctgctcgc tttcttgctg
     1801 tcccatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact gggggatatt
     1861 atgaagggcc ttgagcatct ggattctgcc tagcgctaag cttaacacga gccatagata
     1921 gaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     1981 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa
     2041 tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat
     2101 atctgtggta agcagttcct gccccggctc agggccaaga acagttggaa cagcagaata
     2161 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     2221 tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg
     2281 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     2341 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     2401 tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtgttct caataaaccc
     2461 tcttgcagtt gcatccgact cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     2521 ttgactgccc acctcggggg tctttcattc tcgagcagct tggcgtaatc atggtcatag
     2581 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2641 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2701 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2881 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2941 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3001 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3061 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3601 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3661 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4681 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4741 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     4801 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4861 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4921 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     4981 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     5041 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     5101 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     5161 cagtcacgac gttgtaaaac gacggccagt gaattagtac tctagcttaa gtaacgcc
//
