LOCUS       sequence-304893-        3302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA vaccination plasmid containing SIINFEKL and several CD4+ T cell 
            epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Bresser K, Dijkgraaf FE, Pritchard CEJ, Huijbers IJ, Song JY, Rohr 
            JC, Scheeren FA, Schumacher TN
  TITLE     A mouse model that is immunologically tolerant to reporter and 
            modifier proteins.
  JOURNAL   Commun Biol. 2020 May 29;3(1):273. doi: 10.1038/s42003-020-0979-0.
  PUBMED    32472011
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-29"; volume: "3(1):273. doi"; pages: " 
            10.1038/s42003-020-0979-0"
COMMENT     Sequence Label: pVAX-Help-SIINFEKL
FEATURES             Location/Qualifiers
     source          1..3302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     256..275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        456..835
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        836..1039
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     990..1010
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1084..1103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1084..1102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1547..1564)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1553..1777
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             1950..2744
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1950..2744
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2004..2023)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2614..2633
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      join(3069..3302,1..355)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
       61 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      421 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
      481 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      541 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      601 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      661 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      721 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      781 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      841 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      901 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      961 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     1021 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     1081 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttgcc
     1141 accatgacta gtgccaccgg cagccggacc agcctgctgc tggcctttgg cctgctgtgc
     1201 ctgccctggc tgcaggaagg ctctgccact agtggatccg gccctggccc cggacccggc
     1261 ttcaacaact tcaccgtgtc cttttggctg cgggtgccca aggtgtccgc cagccacctg
     1321 gaaggaccag gacctggacc aggcgccaag tttgtggccg cctggaccct gaaggccgct
     1381 gctggaccag gtcctgggcc cggcgcctgg ctggaagccc aggaagagga agaagtcggc
     1441 ttcgggcctg ggcctggacc tggcgagcag ctggagagca tcatcaactt cgagaagctg
     1501 aaggacgaac tgtaatctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     1561 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     1621 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     1681 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     1741 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctactgggcg gttttatgga
     1801 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
     1861 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagctctg
     1921 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     1981 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     2041 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     2101 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     2161 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     2221 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     2281 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     2341 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     2401 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     2461 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     2521 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     2581 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     2641 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     2701 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattatt aacgcttaca
     2761 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatcaggt
     2821 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2881 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata gcacgtgcta
     2941 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3001 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3061 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3121 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3181 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3241 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3301 gt
//
