LOCUS       sequence-30493-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Zwaka TP, Thomson JA
  TITLE     Homologous recombination in human embryonic stem cells.
  JOURNAL   Nat Biotechnol. 2003 Mar . 21(3):319-21.
  PUBMED    12577066
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2003 Mar . 21(3):319-21."
COMMENT     Sequence Label: HPRT
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    292..340
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     365..384
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 cgacctcgac ctgcagccaa gcttgggtcg tggacctgac cccacgcaac gcccaaaata
       61 ataaccccca cgaaccataa accattcccc atgggggacc ccgtccctaa cccacggggc
      121 cagtggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
      181 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
      241 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
      301 acccaacacc cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc
      361 ggtattgtct ccttccgtgt ttcagttagc ctcccccatc tcccgggcaa acgtgcgcgc
      421 caggtcgcat atcgtcggta tggagccggg ggtggtgacg tgggtctgga ccatcccgga
      481 ggtaagttgc agcagggcgt cccggcagcc ggcgggcgat tggtcgtaat ccaggataaa
      541 gacgtgcatg gaacggaggc gtttggccaa gacgtccaag gcccaggcaa acacgttatn
      601 naggtcgcgt tgggggncag caactcgggg gcccgaaaca gggtaaaata acgtgtcccc
      661 cgatatgggg tcgtgggccc gcgtttgctc tggggcctcg gcacccctgt gggcgggcac
      721 ggccgtcccc gaaaagcttg gtctcccagt ccctccctnc cacgacccgc ccgcactgca
      781 gataccgcac c
//
