LOCUS       sequence-304930-        8393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutisite Gateway mediated vector construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, 
            Nakamura M, Sasaki E, Shiozawa S, Okano H
  TITLE     A versatile toolbox for knock-in gene targeting based on the 
            Multisite Gateway technology.
  JOURNAL   PLoS One. 2019 Aug 27;14(8):e0221164. doi: 
            10.1371/journal.pone.0221164. eCollection 2019.
  PUBMED    31454364
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-27"; volume: "14(8):e0221164. doi"; pages: " 
            10.1371/journal.pone.0221164. eCollection 2019"
COMMENT     Sequence Label: pPB-DEST-R5R4-pPuroTK
FEATURES             Location/Qualifiers
     source          1..8393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1021..1144
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1573..1878)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1675..1694)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2198..2881)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     2796..2815
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2882..2984)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(3162..3285)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3472..3971
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3507..3530)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3904..3923
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3995..4591
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3995..4014)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4491..4511
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4598..5593
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     repeat_region   6119..6181
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6205..6223)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6244..6260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6244..6260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6257..6279)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6268..6284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6292..6298,6299..6316,6317..6322))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6337..6358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6475..6492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6646..7234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6726..6745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7405..8196,8197..8265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8028..8047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8266..8370)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gaggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatgatcc actagtaacg gccgccagtg tgctggaatt cgcggccgct agagtcgggg
     1021 caactttgta tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     1081 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     1141 tatgaatcaa ctacttagat ggtattagtg acctgtagtc gaccgacagc cttccaaatg
     1201 ttcttcgggt gatgctgcca acttagtcga ccgacagcct tccaaatgtt cttctcaaac
     1261 ggaatcgtcg tatccagcct actcgctatt gtcctcaatg ccgtattaaa tcataaaaag
     1321 aaataagaaa aagaggtgcg agcctctttt ttgtgtgaca aaataaaaac atctacctat
     1381 tcatatacgc tagtgtcata gtcctgaaaa tcatctgcat caagaacaat ttcacaactc
     1441 ttatactttt ctcttacaag tcgttcggct tcatctggat tttcagcctc tatacttact
     1501 aaacgtgata aagtttctgt aatttctact gtatcgacct gcagactggc tgtgtataag
     1561 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc
     1621 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc
     1681 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga
     1741 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt
     1801 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt
     1861 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt
     1921 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac
     1981 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat
     2041 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat
     2101 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa
     2161 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccacgcggc
     2221 gtttacgccc cccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2281 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2341 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2401 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2461 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2521 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2581 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2641 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     2701 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2761 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2821 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2881 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2941 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     3001 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     3061 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     3121 tagtcgacta caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt
     3181 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     3241 ttatatcatt ttacgtttct cgttcaactt ttctatacaa agttgccccg acctgcaggc
     3301 atgcaagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
     3361 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
     3421 cataggcttg gttatgccgg tactgccggg cctcttgcgg ggaattctac cgggtagggg
     3481 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     3541 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     3601 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     3661 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     3721 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     3781 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccc
     3841 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     3901 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     3961 cgggcctttc gaccgatcat caagcttgat cctcatgacc gagtacaagc ccacggtgcg
     4021 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4081 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4141 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4201 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4261 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4321 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4381 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4441 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4501 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4561 cacctggtgc atgacccgca agcccggtgc cggatccatg cccacgctac tgcgggttta
     4621 tatagacggt ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg
     4681 ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcggg tgctgggggc
     4741 ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc
     4801 ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc
     4861 cgtgaccgac gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc
     4921 gcccccggcc ctcaccctca tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc
     4981 ggccgcgcgg taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct
     5041 catcccgccg accttgcccg gcaccaacat cgtgcttggg gcccttccgg aggacagaca
     5101 catcgaccgc ctggccaaac gccagcgccc cggcgagcgg ctggacctgg ctatgctggc
     5161 tgcgattcgc cgcgtttacg ggctacttgc caatacggtg cggtatctgc agtgcggcgg
     5221 gtcgtggcgg gaggactggg gacagctttc ggggacggcc gtgccgcccc agggtgccga
     5281 gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg
     5341 ggcccccgag ttgctggccc ccaacggcga cctgtataac gtgtttgcct gggccttgga
     5401 cgtcttggcc aaacgcctcc gttccatgca cgtctttatc ctggattacg accaatcgcc
     5461 cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga cccacgtcac
     5521 cacccccggc tccataccga cgatatgcga cctggcgcgc acgtttgccc gggagatggg
     5581 ggaggctaac tgagctctag cttatcgact cgccgatagt ggaaaccgac gccccagcac
     5641 tcgtccgagg gcaaaggaat agtcgagaaa ttgatgatct attaaacaat aaagatgtcc
     5701 acatggaagt ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt
     5761 tgaatggaag gattggagct acgggggtgg gggtggggtg ggattagata aatgcctgct
     5821 ctttactgaa ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat
     5881 ttaaacaagc aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc
     5941 catcagatcc tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat
     6001 aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat
     6061 aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca
     6121 tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg
     6181 gttaaagagc tccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
     6241 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6301 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6481 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6541 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6841 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6961 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7021 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7081 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7141 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7201 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7261 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7321 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7381 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7441 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7501 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7561 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7621 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7681 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7741 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7801 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7861 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7921 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7981 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8041 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8101 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8161 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacgggaa ggcaaaatgc
     8221 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8281 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8341 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
