LOCUS       sequence-30500-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, 
            Skarnes WC, Reiter JF
  TITLE     Floxin, a resource for genetically engineering mouse ESCs.
  JOURNAL   Nat Methods. 2010 Jan . 7(1):50-2.
  PUBMED    19966808
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Jan . 7(1):50-2."
COMMENT     Sequence Label: pFloxin-TAP
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(10..57)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    75..209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(99..118)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     153..172
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(398..463)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(470..490)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(518..535)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcgtcgacg aagttcctat actttctaga gaataggaac ttcggaatag gaacttctcg
       61 acgcggtacc cccggatcta gacatgataa gatacattga tgagtttgga caaaccacaa
      121 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      181 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      241 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac agatgtgata
      301 tggctgatta tgatcattac ttatctagcc ccccctcgag gtcgctctag agcgacccca
      361 ctgggctgta ggtggtggat ccgctagcga tatcctactt gtcatcgtca tccttgtagt
      421 cgatgtcatg atctttataa tcaccgtcat ggtctttgta gtcgagctca ccctgaaaat
      481 acaaattctc gctagcagta gttggaatat cataatcgtg atggtgatgg tgatgactag
      541 tggcgcgccg catgcaccgg tacctggttg tcatggagga gaaagggcag aggttactgg
      601 ttgctggagt ctagctactt atccacaacc aacgcaccca agctagcttg aggttgcaga
      661 tactgggggt gggggggggg g
//
