LOCUS       sequence-305001-       10915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic 
            H3F3A G34R, PDGFRA D842V, and TRP53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Kim GB, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, 
            Dutra-Clarke M, Levy R, Watkins A, Park H, Abbasi Akhtar A, Linesch 
            PW, Kobritz N, Chandra SS, Grausam K, Ayala-Sarmiento A, Molina J, 
            Sedivakova K, Hoang K, Tsyporin J, Gareau DS, Filbin MG, Bannykh S, 
            Santiskulvong C, Wang Y, Tang J, Suva ML, Chen B, Danielpour M, 
            Breunig JJ
  TITLE     Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, 
            Stably Integrated Transgenic Elements.
  JOURNAL   Cell. 2019 Sep 19;179(1):251-267.e24. doi: 
            10.1016/j.cell.2019.08.013.
  PUBMED    31539496
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08"; journalName: "Cell"; date: "2019-09-19- 
            19"; volume: "179"; issue: "1"; pages: "251-267.e24"
COMMENT     Sequence Label: MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V ...
FEATURES             Location/Qualifiers
     source          1..10915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    32..493
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    641..775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    895..1029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1149..1283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1312..1345)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1380..1396)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1381..1397)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     regulatory      1413..1422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1845..1847,1848..2558)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1845..1847,1848..2558)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1887..1908)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2148..2167)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2495..2516
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2601..2657
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2991..3002
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5943..5999
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6372..6383)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7179..7220
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7233..7821
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7286..7306)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7704..7715)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7906..7925)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7971..8026
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8025..8044)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    8366..8399
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8418..8434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8418..8434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8431..8453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8442..8458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8466..8472,8473..8490,8491..8496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8511..8532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8591..8786
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8637..8772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8792..8926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8994..9011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9165..9753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9245..9264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9924..10715,10716..10784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10547..10566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10785..10889)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagtggatcc gctgtaagtc tgcagaaatt gatgatctat
       61 taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg
      121 tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg
      181 tgggattaga taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg
      241 tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc
      301 tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt
      361 cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa
      421 cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct
      481 aattccatca gaagcttgca gatctgcgac tctagaggat ctgcgactct agaggatcat
      541 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      601 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      721 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgcga
      781 ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      841 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1021 gtctggatct gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac
     1081 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1141 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1201 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1261 atgtatctta tcatgtctgg atccccatca agctgatccg gaacccttaa tataacttcg
     1321 tatagcatac attatacgaa gttattaggt ccctcgacct gcagcccaag ctagcttatc
     1381 gataccgtcg acctcgaggg ggggaattcg ccgccaccat ggctcgtaca aagcagactg
     1441 cccgcaaatc caccggtggt aaagcaccca ggaaacaact ggctacaaaa gccgctcgca
     1501 agagtgcgcc ctctactgga agggtgaaga aacctcatcg ttacaggcct ggtactgtgg
     1561 ccctccgtga aatcagacgc tatcagaagt ccactgaact tctgatccgc aagctcccct
     1621 ttcagcgtct ggtgcgagaa attgctcagg acttcaaaac agatctgcgc ttccagagtg
     1681 cagctattgg tgctttgcag gaggcaagtg aggcctatct ggttggcctt tttgaagata
     1741 ccaatctgtg tgctatccat gccaaacgtg taacaattat gccaaaagat atccagctag
     1801 cacgccgcat acgcggagaa cgtgcttcga gaccggtagc tactgtgagc aagggcgagg
     1861 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1921 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1981 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2041 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2101 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2161 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2221 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2281 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2341 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2401 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2461 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2521 ccgccgggat cactctcggc atggacgagc tgtacaaggg cggcgatacc tatcggtata
     2581 ttggatccgg aggatccgga gccacgaact tctctctgtt aaagcaagca ggagacgtgg
     2641 aagaaaaccc cggtcctatg gggacctccc accaggtctt tctggtcctc agctgtctcc
     2701 tcacagggcc gggcctcatc tcctgccagc tcttattacc ctctatcctc ccaaacgaga
     2761 atgagaagat tgtgcagctg aattcgtcct tttctctgag atgcgttggg gagagtgaag
     2821 tgagctggca gcaccccatg tctgaagaag acgaccccaa cgtggaaatc agaagtgagg
     2881 agaacaacag tggcctcttt gtcacagtgc tggaagtggt taacgcctcc gcagcccaca
     2941 ccggatggta cacttgctac tacaaccaca ctcagacgga tgagagtgag atcgaaggca
     3001 ggcacattta catctatgta ccagacccag acatggcctt tgtgcctctc gggatgaccg
     3061 attccttagt catcgtggaa gaggatgact ctgccatcat accttgccgc accacagatc
     3121 cggagactca agtaaccttg cacaataacg ggaggctggt gcctgcctcc tatgacagca
     3181 ggcagggctt caacggaacc ttcagcgtgg ggccttacat ctgtgaggcc accgtcaaag
     3241 ggaggacgtt caagaccagc gagtttaatg tttatgcctt gaaagcaacg tcagaactga
     3301 atctggagat ggacgcacgc cagactgtgt ataaggcagg agaaacgatc gtggtgacct
     3361 gtgccgtctt taacaacgag gtcgttgacc tgcagtggac ttaccctgga gaagtgagaa
     3421 acaaaggtat caccatgctg gaggagatca aactcccatc catcaaactg gtgtacactt
     3481 tgaccgtccc caaggccacg gtgaaggaca gtggagagta tgaatgtgct gcccgtcagg
     3541 ccactaaaga ggtcaaggaa atgaagagag tcaccatttc tgtccacgag aaaggcttcg
     3601 tcgagattga gcccaccttt ggccagctgg aagctgtcaa cttgcacgaa gtcagagagt
     3661 tcgtggtgga ggtgcaggcc tacccgacgc ccaggatatc gtggctgaag gacaacttga
     3721 ctctgataga gaatctcacc gagatcacca ctgatgtaca gaagagtcag gaaacaaggt
     3781 atcagagcaa attaaagctg atccgggcta aggaagaaga cagtggccat tacaccatta
     3841 tagttcaaaa tgaggatgac gtgaagagtt acacgtttga gctgtcaacc ctagttcctg
     3901 catccatttt ggatctcgta gatgaccacc atggctctgg cgggggacag actgtgaggt
     3961 gtacagccga aggcacccct cttccagaaa tcgactggat gatctgcaag catattaaga
     4021 aatgtaataa tgacacttcg tggacagttt tggccagcaa tgtctcaaat attatcacag
     4081 agctccctcg ccgtggcagg agtaccgtgg agggacgagt gtccttcgcc aaagtggaag
     4141 agaccatcgc agttcgatgc ctggcaaaga acaacctcag cgttgtggcc cgtgagctga
     4201 agctggtggc tcccactctg cgatctgaac tcacagtggc ggccgcagtg ttggtgctgt
     4261 tggtgattgt cattgtctct ctcatcgtcc tggtggtcat ttggaagcag aaaccacggt
     4321 atgaaattcg ctggagggtt atcgagtcaa tcagccccga tggacatgag tatatctatg
     4381 tggaccccat gcagttgcct tacgactcca gatgggaatt cccgagagat ggcctcgtgc
     4441 ttggtcggat tttgggatcc ggtgcatttg ggaaagtggt cgaaggtaca gcttatggat
     4501 taagccggtc ccaacctgta atgaaggtgg ctgtgaagat gctcaaaccc acagccaggt
     4561 ctagtgaaaa gcaggctctc atgtctgagc tgaagataat gactcacctg gggccacatt
     4621 tgaacattgt gaacttgctg ggagcctgca ccaagtcagg tcccatttac atcatcacag
     4681 aatactgctt ctatggggat ttggtcaact acttgcataa gaacagagat agcttcatga
     4741 gccaacaccc agagaagccg aagaaagacc tggacatctt tggattgaat cctgcagacg
     4801 agagcacaag aagttatgtg attttgtctt ttgaaaataa cggcgactac atggacatga
     4861 agcaagctga taccacacag tatgtcccca tgcttgaaag gaaagaggtt tctaaatact
     4921 ctgacatcca gagatcgctg tacgatcggc cagcctccta caagaagaaa tccatgctag
     4981 actcagaagt caaaaacctc ctttcggacg atgactccga gggtctgact ttgctggatc
     5041 tattgagctt cacctatcaa gttgctcgag gaatggaatt tttggcttcg aaaaattgtg
     5101 tccaccggga cctggctgcc cgcaacgtcc tcctggcgca aggaaaaatt gtgaagatct
     5161 gtgattttgg cctggccaga gtcatcatgc acgattccaa ctacgtgtca aaaggcagta
     5221 ctttcctgcc tgtgaagtgg atggcacccg agagcatctt cgacaacctc tacaccacgc
     5281 tgagtgacgt ctggtcctat ggcattctgc tctgggaaat cttttccctt ggtggcacac
     5341 cctatcctgg catgatggtc gattctactt tctacaataa gatcaagagt ggataccgga
     5401 tggccaaacc tgaccatgcc accagtgaag tctatgagat catggtgcag tgctggaaca
     5461 gtgagcccga gaagagaccc tccttctacc acctcagcga gatagtggag aacctgttgc
     5521 cgggacaata caagaagagt tatgaaaaga ttcacctgga cttcctaaag agtgaccatc
     5581 cggccgtggc acgcatgcgg gtggactctg ataatgcgta catcggtgtc acttacaaaa
     5641 atgaagagga taagctgaag gactgggaag gtggcctgga cgaacagaga ctgagcgctg
     5701 acagtggcta catcatcccc ctgccagaca ttgaccctgt tccagaggag gaagacctgg
     5761 gcaagaggaa cagacacagc tcacagactt cggaagagag tgccatcgag acaggttcca
     5821 gtagttccac cttcatcaag agagaggacg agaccatcga ggacatcgac atgatggatg
     5881 acattggtat agattcctcg gacctggtgg aggacagctt cctgggcagt ggagggagtg
     5941 gagcaaccaa tttttcactc ctgaagcaag ctggggatgt agaggagaat cctgggccta
     6001 tgactgccat ggaggagtca cagtcggata tcagcctcga gctccctctg agccaggaga
     6061 cattttcagg cttatggaaa ctacttcctc cagaagatat cctgccatca cctcactgca
     6121 tggacgatct gttgctgccc caggatgttg aggagttttt tgaaggccca agtgaagccc
     6181 tccgagtgtc aggagctcct gcagcacagg accctgtcac cgagacccct gggccagtgg
     6241 cccctgcccc agccactcca tggcccctgt catcttttgt cccttctcaa aaaacttacc
     6301 agggcaacta tggcttccac ctgggcttcc tgcagtctgg gacagccaag tctgttatgt
     6361 gcacgtactc tcctcccctc aataagctat tctgccagct ggcgaagacg tgccctgtgc
     6421 agttgtgggt cagcgccaca cctccagctg ggagccgtgt ccgcgccatg gccatctaca
     6481 agaagtcaca gcacatgacg gaggtcgtga gacgctgccc ccaccatgag cgctgctccg
     6541 atggtgatgg cctggctcct ccccagcatc ttatccgggt ggaaggaaat ttgtatcccg
     6601 agtatctgga agacaggcag acttttcgcc acagcgtggt ggtaccttat gagccacccg
     6661 aggccggctc tgagtatacc accatccact acaagtacat gtgtaatagc tcctgcatgg
     6721 ggggcatgaa ccgccgacct atccttacca tcatcacact ggaagactcc agtgggaacc
     6781 ttctgggacg ggacagcttt gaggttcatg tttgtgcctg ccctgggaga gaccgccgta
     6841 cagaagaaga aaatttccgc aaaaaggaag tcctttgccc tgaactgccc ccagggagcg
     6901 caaagagagc gctgcccacc tgcacaagcg cctctccccc gcaaaagaaa aaaccacttg
     6961 atggagagta tttcaccctc aagatccgcg ggcgtaaacg cttcgagatg ttccgggagc
     7021 tgaatgaggc cttagagtta aaggatgccc atgctacaga ggagtctgga gacagcaggg
     7081 ctcactccag ctacctgaag accaagaagg gccagtctac ttcccgccat aaaaaaacaa
     7141 tggtcaagaa agtggggcct gactcagacg gtggctcagg taagcctatc cctaaccctc
     7201 tcctcggtct cgattctacg taagcggccg ctaatcaacc tctggattac aaaatttgtg
     7261 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7321 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7381 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7441 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7501 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7561 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7621 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7681 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7741 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7801 ccctttgggc cgcctccccg caggccgcac tcctcaggtg caggctgcct atcagaaggt
     7861 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     7921 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     7981 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     8041 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     8101 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     8161 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     8221 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     8281 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     8341 cttatggaga tccctcgacc tgcaggaagt tcctattctc tagaaagtat aggaacttca
     8401 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8461 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8521 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8581 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8701 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8761 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
     8821 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8881 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
     8941 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9361 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9601 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9901 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9961 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10021 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10081 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10141 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10201 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctg
//
