LOCUS       sequence-305018-        8870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutisite Gateway mediated vector construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, 
            Nakamura M, Sasaki E, Shiozawa S, Okano H
  TITLE     A versatile toolbox for knock-in gene targeting based on the 
            Multisite Gateway technology.
  JOURNAL   PLoS One. 2019 Aug 27;14(8):e0221164. doi: 
            10.1371/journal.pone.0221164. eCollection 2019.
  PUBMED    31454364
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-27"; volume: "14(8):e0221164. doi"; pages: " 
            10.1371/journal.pone.0221164. eCollection 2019"
COMMENT     Sequence Label: pPB-DEST-R5R4-pHygTK
FEATURES             Location/Qualifiers
     source          1..8870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1021..1144
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1573..1878)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1675..1694)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2198..2881)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     2796..2815
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2882..2984)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(3162..3285)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3472..3971
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3507..3530)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3904..3923
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5075..6070
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     repeat_region   6596..6658
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6682..6700)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6721..6737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6721..6737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6734..6756)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6745..6761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6769..6775,6776..6793,6794..6799))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6814..6835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6952..6969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7123..7711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7203..7222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7882..8673,8674..8742))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8505..8524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8743..8847)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gaggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatgatcc actagtaacg gccgccagtg tgctggaatt cgcggccgct agagtcgggg
     1021 caactttgta tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     1081 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     1141 tatgaatcaa ctacttagat ggtattagtg acctgtagtc gaccgacagc cttccaaatg
     1201 ttcttcgggt gatgctgcca acttagtcga ccgacagcct tccaaatgtt cttctcaaac
     1261 ggaatcgtcg tatccagcct actcgctatt gtcctcaatg ccgtattaaa tcataaaaag
     1321 aaataagaaa aagaggtgcg agcctctttt ttgtgtgaca aaataaaaac atctacctat
     1381 tcatatacgc tagtgtcata gtcctgaaaa tcatctgcat caagaacaat ttcacaactc
     1441 ttatactttt ctcttacaag tcgttcggct tcatctggat tttcagcctc tatacttact
     1501 aaacgtgata aagtttctgt aatttctact gtatcgacct gcagactggc tgtgtataag
     1561 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc
     1621 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc
     1681 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga
     1741 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt
     1801 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt
     1861 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt
     1921 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac
     1981 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat
     2041 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat
     2101 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa
     2161 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccacgcggc
     2221 gtttacgccc cccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2281 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2341 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2401 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2461 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2521 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2581 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2641 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     2701 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2761 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2821 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2881 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2941 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     3001 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     3061 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     3121 tagtcgacta caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt
     3181 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     3241 ttatatcatt ttacgtttct cgttcaactt ttctatacaa agttgccccg acctgcaggc
     3301 atgcaagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
     3361 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
     3421 cataggcttg gttatgccgg tactgccggg cctcttgcgg ggaattctac cgggtagggg
     3481 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     3541 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     3601 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     3661 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     3721 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     3781 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     3841 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     3901 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     3961 cgggcctttc gaccgatcat caagcttgat cctcatgaaa aagcctgaac tcaccgcgac
     4021 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     4081 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     4141 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     4201 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     4261 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     4321 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     4381 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     4441 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     4501 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     4561 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     4621 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     4681 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     4741 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     4801 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     4861 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     4921 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtcgcgt ctgcgttcga
     4981 ccaggctgcg cgttctcgcg gccatagcaa ccgacgtacg gcgttgcgcc ctcgccggca
     5041 gcaagaagcc acggaagtcc gcccggagca gaaaatgccc acgctactgc gggtttatat
     5101 agacggtccc cacgggatgg ggaaaaccac caccacgcaa ctgctggtgg ccctgggttc
     5161 gcgcgacgat atcgtctacg tacccgagcc gatgacttac tggcgggtgc tgggggcttc
     5221 cgagacaatc gcgaacatct acaccacaca acaccgcctc gaccagggtg agatatcggc
     5281 cggggacgcg gcggtggtaa tgacaagcgc ccagataaca atgggcatgc cttatgccgt
     5341 gaccgacgcc gttctggctc ctcatatcgg gggggaggct gggagctcac atgccccgcc
     5401 cccggccctc accctcatct tcgaccgcca tcccatcgcc gccctcctgt gctacccggc
     5461 cgcgcggtac cttatgggca gcatgacccc ccaggccgtg ctggcgttcg tggccctcat
     5521 cccgccgacc ttgcccggca ccaacatcgt gcttggggcc cttccggagg acagacacat
     5581 cgaccgcctg gccaaacgcc agcgccccgg cgagcggctg gacctggcta tgctggctgc
     5641 gattcgccgc gtttacgggc tacttgccaa tacggtgcgg tatctgcagt gcggcgggtc
     5701 gtggcgggag gactggggac agctttcggg gacggccgtg ccgccccagg gtgccgagcc
     5761 ccagagcaac gcgggcccac gaccccatat cggggacacg ttatttaccc tgtttcgggc
     5821 ccccgagttg ctggccccca acggcgacct gtataacgtg tttgcctggg ccttggacgt
     5881 cttggccaaa cgcctccgtt ccatgcacgt ctttatcctg gattacgacc aatcgcccgc
     5941 cggctgccgg gacgccctgc tgcaacttac ctccgggatg gtccagaccc acgtcaccac
     6001 ccccggctcc ataccgacga tatgcgacct ggcgcgcacg tttgcccggg agatggggga
     6061 ggctaactga gctctagctt atcgactcgc cgatagtgga aaccgacgcc ccagcactcg
     6121 tccgagggca aaggaatagt cgagaaattg atgatctatt aaacaataaa gatgtccaca
     6181 tggaagtttt tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga
     6241 atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat gcctgctctt
     6301 tactgaaggc tctttactat tgctttatga taatgtttca tagttggata tcataattta
     6361 aacaagcaaa accaaattaa gggccagctc attcctccca ctcatgatct atagatccat
     6421 cagatccttt gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa
     6481 taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa
     6541 acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc
     6601 gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt
     6661 aaagagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
     6721 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6781 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6841 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6901 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7561 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acgggaaggc aaaatgccgc
     8701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
