LOCUS       sequence-30502-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, 
            Skarnes WC, Reiter JF
  TITLE     Floxin, a resource for genetically engineering mouse ESCs.
  JOURNAL   Nat Methods. 2010 Jan . 7(1):50-2.
  PUBMED    19966808
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Jan . 7(1):50-2."
COMMENT     Sequence Label: pFloxin-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(23..70)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    88..222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(112..131)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     166..185
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(467..488)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgattacga attcgcgtcg acgaagttcc tatactttct agagaatagg aacttcggaa
       61 taggaacttc tcgacgcggt acccccggat ctagacatga taagatacat tgatgagttt
      121 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      181 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      241 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      301 tacagatgtg atatggctga ttatgatcat tacttatcta gccccccctc gaggtcgctc
      361 tagagcgacc ccactgggct gtaggtggtg gatccgctag cgatggcctc gagcggccgc
      421 tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      481 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      541 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      601 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
      661 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
      721 tgtgccccag gatgttgccg tcctccttga agt
//
