LOCUS       sequence-305022-        9396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutisite Gateway mediated vector construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, 
            Nakamura M, Sasaki E, Shiozawa S, Okano H
  TITLE     A versatile toolbox for knock-in gene targeting based on the 
            Multisite Gateway technology.
  JOURNAL   PLoS One. 2019 Aug 27;14(8):e0221164. doi: 
            10.1371/journal.pone.0221164. eCollection 2019.
  PUBMED    31454364
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-27"; volume: "14(8):e0221164. doi"; pages: " 
            10.1371/journal.pone.0221164. eCollection 2019"
COMMENT     Sequence Label: CSIV-DEST
FEATURES             Location/Qualifiers
     source          1..9396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             152..332
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(358..375)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    364..588
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      634..1062
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(721..740)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     931..952
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1071..1091)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1076..1405
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1256..1391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1318..1337
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1453..1500
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1519..1893
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2023..2144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2060..2079)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2114..2133
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2193..2209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2193..2209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2206..2228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2217..2233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2241..2247,2248..2265,2266..2271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2286..2307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2424..2441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2595..3183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2675..2694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3354..4145,4146..4214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3977..3996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4215..4319)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4394..4413)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4585..4964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4966..5164
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5119..5139
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5182..5362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5409..5534
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6027..6260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6445..6489
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6763..6880
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6928..7052
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7077..7082,7083..7100,7101..7107)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7161..7820
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7227..7246)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8162..8467
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8346..8365
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8508..8632)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8676..9264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8729..8749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9147..9158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
       61 ggggactgga agggctaatt cactcccaac gaagacaaga tatcataact tcgtatagca
      121 tacattatac gaagttattc cggactgtac tgggtctctc tggttagacc agatctgagc
      181 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      241 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      301 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
      361 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      421 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      481 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      541 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
      601 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
      661 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      721 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      781 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      841 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      901 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      961 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     1021 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     1081 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     1141 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     1201 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     1261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     1321 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     1381 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     1441 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     1501 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     1561 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     1621 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     1681 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     1741 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     1801 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     1861 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     1921 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     1981 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     2041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     2161 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4321 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     4381 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     4441 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     4501 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     4561 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     4621 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     4681 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     4741 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     4801 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     4861 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     4921 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     4981 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5041 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5101 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     5161 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5221 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5281 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5341 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     5401 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     5461 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     5521 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     5581 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     5641 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     5701 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     5761 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     5821 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     5881 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     5941 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6001 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6061 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6121 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6181 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     6241 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     6301 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     6361 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     6421 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     6481 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     6541 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     6601 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     6661 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     6721 cgattagtga acggatctac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     6781 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     6841 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     6901 cagaaattca ctttgaattc tagcatcaca agtttgtaca aaaaagctga acgagaaacg
     6961 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     7021 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
     7081 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
     7141 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     7201 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     7261 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     7321 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     7381 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     7441 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     7501 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     7561 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     7621 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     7681 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     7741 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     7801 agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg
     7861 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     7921 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     7981 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     8041 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     8101 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
     8161 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     8221 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
     8281 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     8341 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     8401 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     8461 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
     8521 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     8581 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgctac
     8641 cggtctcgaa acatcgaggg atcaagctta tcgataatca acctctggat tacaaaattt
     8701 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     8761 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     8821 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     8881 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     8941 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     9001 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     9061 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     9121 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     9181 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     9241 tctccctttg ggccgcctcc ccgcatcgat accgtcgaga cctagaaaaa catggagcaa
     9301 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     9361 aggaggaggt gggttttcca gtcacacctc aggtac
//
