LOCUS       sequence-30503-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, 
            Skarnes WC, Reiter JF
  TITLE     Floxin, a resource for genetically engineering mouse ESCs.
  JOURNAL   Nat Methods. 2010 Jan . 7(1):50-2.
  PUBMED    19966808
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Jan . 7(1):50-2."
COMMENT     Sequence Label: pFloxin-Ofd1-Myc
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..94)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    112..246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(136..155)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     190..209
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(590..619)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atttcacaca ggaacagcta tgacatgatt acgaattcgc gtcgacgaag ttcctatact
       61 ttctagagaa taggaacttc ggaataggaa cttctcgacg cggtaccccc ggatctagac
      121 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      181 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      241 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      301 gttttttaaa gcaagtaaaa cctctacaga tgtgatatgg ctgattatga tcattactta
      361 tctagccccc cctcgaggtc gctctagagc gaccccactg ggctgtaggt ggtggatccg
      421 ctagcgatct cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg
      481 cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg gagggagagg
      541 ggcggatcca ctagtaacgg ccgccagtgt gctggaattc gcccttttac aagtcctctt
      601 cagaaatgag cttttgctcc cacatgtcat ctggttcttc atgagaaaat cctggagaac
      661 tttcatcctt gtcactaggc atcatcatgt ccactagtga gcattctttg cccgattttt
      721 tggagctctt atctgcattt gattcttcct gtctctgctg gataattttc atgtattttt
      781 ctaaagtatt ttcacaatga gctgatttgt ctttctggtc ttgaacactc ttttctaatt
      841 cttcttccat ctccatcttc agtgactctt caatcatcct ccgctcctg
//
