LOCUS       sequence-305035-        7746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutisite Gateway mediated vector construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, 
            Nakamura M, Sasaki E, Shiozawa S, Okano H
  TITLE     A versatile toolbox for knock-in gene targeting based on the 
            Multisite Gateway technology.
  JOURNAL   PLoS One. 2019 Aug 27;14(8):e0221164. doi: 
            10.1371/journal.pone.0221164. eCollection 2019.
  PUBMED    31454364
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-27"; volume: "14(8):e0221164. doi"; pages: " 
            10.1371/journal.pone.0221164. eCollection 2019"
COMMENT     Sequence Label: pENTR-L3-Csy4-FokI-dCas9-L2
FEATURES             Location/Qualifiers
     source          1..7746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(201..220)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             212..1021
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(279..298)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1164..1752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1653..1672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1887..1914)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2006..2092)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    2146..2241
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      2270..2279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2840..2893
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2897..2917
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2924..3511
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             join(3527..6925,6926..6928,6929..7525,7526,7527..7529,
                     7530..7531,7532..7534,7535..7627)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3527..3550,3551..3553,3554..6040,6041..6043,
                     6044..7627)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7634..7654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7661..7726
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(join(7736..7746,1..89))
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca
      121 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      181 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
      241 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
      301 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
      361 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
      421 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      481 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
      541 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
      601 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
      661 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
      721 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
      781 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
      841 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
      901 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
      961 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     1021 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     1081 cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct
     1861 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     1921 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     1981 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     2041 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     2101 cagttcccta ctctcgcgtt aacgctagca tggatctcgg gccccaaata atgattttat
     2161 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc
     2221 caactttgta taataaagtt gggtaccgcg ggcccgggat ccaccggtcg ccaccatggg
     2281 tgatcattat ctggatattc ggctgaggcc tgatccagag ttcccacctg cgcagctgat
     2341 gtctgtcctt tttggcaaac ttcatcaggc cctggttgcc cagggcggag atcggatagg
     2401 ggtaagcttt ccagacctcg acgaaagccg gagccgcctg ggagaacgcc tgcggatcca
     2461 cgcttctgcc gacgatctga gagccttgct ggcaaggcca tggcttgagg ggctccggga
     2521 tcacctgcag tttggcgaac ccgccgttgt tccccaccca accccttatc ggcaggtgtc
     2581 tagagtgcag gccaaatcta atccagaacg gctgcgacgg cgactcatgc ggcgacatga
     2641 tcttagcgag gaagaggccc gaaaaagaat ccctgatacc gtggcccgcg cccttgactt
     2701 gccttttgtc acactgcggt cccagagtac ggggcagcat ttcagacttt tcattcgaca
     2761 cgggccactg caagttaccg ccgaagaagg aggctttact tgttatggac tctccaaggg
     2821 aggtttcgtg ccctggtttg agggcagagg aagtctgtta acatgcggtg acgtcgagga
     2881 gaatcctggc ccaatgccta agaagaagcg gaaggtgagc agccaacttg tgaagtctga
     2941 actcgaggag aaaaaatcag agttgagaca caagttgaag tacgtgccac acgaatacat
     3001 cgagcttatc gagatcgcca gaaacagtac ccaggatagg atccttgaga tgaaagtcat
     3061 ggagttcttt atgaaggtct acggttatag aggaaagcac cttggcggta gcagaaagcc
     3121 cgatggcgcc atctatactg tcggatctcc tatcgattat ggggtgatcg tggataccaa
     3181 agcttactca ggcgggtaca acttgcccat aggacaagcc gacgagatgc agcggtatgt
     3241 cgaagagaac cagacgcgca acaagcacat caaccccaat gaatggtgga aagtgtaccc
     3301 aagtagtgtg actgagttca agttcctgtt tgtctccggc cactttaagg gcaattataa
     3361 agctcagctc actagactca atcacatcac aaactgcaac ggagctgtgt tgtcagtgga
     3421 ggagctcctg attggaggcg agatgatcaa agccggcacc cttacactgg aggaggtgcg
     3481 gcggaagttc aacaatggag agatcaactt cggtggcggt ggatccgata aaaagtattc
     3541 tattggttta gccatcggca ctaattccgt tggatgggct gtcataaccg atgaatacaa
     3601 agtaccttca aagaaattta aggtgttggg gaacacagac cgtcattcga ttaaaaagaa
     3661 tcttatcggt gccctcctat tcgatagtgg cgaaacggca gaggcgactc gcctgaaacg
     3721 aaccgctcgg agaaggtata cacgtcgcaa gaaccgaata tgttacttac aagaaatttt
     3781 tagcaatgag atggccaaag ttgacgattc tttctttcac cgtttggaag agtccttcct
     3841 tgtcgaagag gacaagaaac atgaacggca ccccatcttt ggaaacatag tagatgaggt
     3901 ggcatatcat gaaaagtacc caacgattta tcacctcaga aaaaagctag ttgactcaac
     3961 tgataaagcg gacctgaggt taatctactt ggctcttgcc catatgataa agttccgtgg
     4021 gcactttctc attgagggtg atctaaatcc ggacaactcg gatgtcgaca aactgttcat
     4081 ccagttagta caaacctata atcagttgtt tgaagagaac cctataaatg caagtggcgt
     4141 ggatgcgaag gctattctta gcgcccgcct ctctaaatcc cgacggctag aaaacctgat
     4201 cgcacaatta cccggagaga agaaaaatgg gttgttcggt aaccttatag cgctctcact
     4261 aggcctgaca ccaaatttta agtcgaactt cgacttagct gaagatgcca aattgcagct
     4321 tagtaaggac acgtacgatg acgatctcga caatctactg gcacaaattg gagatcagta
     4381 tgcggactta tttttggctg ccaaaaacct tagcgatgca atcctcctat ctgacatact
     4441 gagagttaat actgagatta ccaaggcgcc gttatccgct tcaatgatca aaaggtacga
     4501 tgaacatcac caagacttga cacttctcaa ggccctagtc cgtcagcaac tgcctgagaa
     4561 atataaggaa atattctttg atcagtcgaa aaacgggtac gcaggttata ttgacggcgg
     4621 agcgagtcaa gaggaattct acaagtttat caaacccata ttagagaaga tggatgggac
     4681 ggaagagttg cttgtaaaac tcaatcgcga agatctactg cgaaagcagc ggactttcga
     4741 caacggtagc attccacatc aaatccactt aggcgaattg catgctatac ttagaaggca
     4801 ggaggatttt tatccgttcc tcaaagacaa tcgtgaaaag attgagaaaa tcctaacctt
     4861 tcgcatacct tactatgtgg gacccctggc ccgagggaac tctcggttcg catggatgac
     4921 aagaaagtcc gaagaaacga ttactccatg gaattttgag gaagttgtcg ataaaggtgc
     4981 gtcagctcaa tcgttcatcg agaggatgac caactttgac aagaatttac cgaacgaaaa
     5041 agtattgcct aagcacagtt tactttacga gtatttcaca gtgtacaatg aactcacgaa
     5101 agttaagtat gtcactgagg gcatgcgtaa acccgccttt ctaagcggag aacagaagaa
     5161 agcaatagta gatctgttat tcaagaccaa ccgcaaagtg acagttaagc aattgaaaga
     5221 ggactacttt aagaaaattg aatgcttcga ttctgtcgag atctccgggg tagaagatcg
     5281 atttaatgcg tcacttggta cgtatcatga cctcctaaag ataattaaag ataaggactt
     5341 cctggataac gaagagaatg aagatatctt agaagatata gtgttgactc ttaccctctt
     5401 tgaagatcgg gaaatgattg aggaaagact aaaaacatac gctcacctgt tcgacgataa
     5461 ggttatgaaa cagttaaaga ggcgtcgcta tacgggctgg ggacgattgt cgcggaaact
     5521 tatcaacggg ataagagaca agcaaagtgg taaaactatt ctcgattttc taaagagcga
     5581 cggcttcgcc aataggaact ttatgcagct gatccatgat gactctttaa ccttcaaaga
     5641 ggatatacaa aaggcacagg tttccggaca aggggactca ttgcacgaac atattgcgaa
     5701 tcttgctggt tcgccagcca tcaaaaaggg catactccag acagtcaaag tagtggatga
     5761 gctagttaag gtcatgggac gtcacaaacc ggaaaacatt gtaatcgaga tggcacgcga
     5821 aaatcaaacg actcagaagg ggcaaaaaaa cagtcgagag cggatgaaga gaatagaaga
     5881 gggtattaaa gaactgggca gccagatctt aaaggagcat cctgtggaaa atacccaatt
     5941 gcagaacgag aaactttacc tctattacct acaaaatgga agggacatgt atgttgatca
     6001 ggaactggac ataaaccgtt tatctgatta cgacgtcgat gccattgtac cccaatcctt
     6061 tttgaaggac gattcaatcg acaataaagt gcttacacgc tcggataaga accgagggaa
     6121 aagtgacaat gttccaagcg aggaagtcgt aaagaaaatg aagaactatt ggcggcagct
     6181 cctaaatgcg aaactgataa cgcaaagaaa gttcgataac ttaactaaag ctgagagggg
     6241 tggcttgtct gaacttgaca aggccggatt tattaaacgt cagctcgtgg aaacccgcca
     6301 aatcacaaag catgttgcac agatactaga ttcccgaatg aatacgaaat acgacgagaa
     6361 cgataagctg attcgggaag tcaaagtaat cactttaaag tcaaaattgg tgtcggactt
     6421 cagaaaggat tttcaattct ataaagttag ggagataaat aactaccacc atgcgcacga
     6481 cgcttatctt aatgccgtcg tagggaccgc actcattaag aaatacccga agctagaaag
     6541 tgagtttgtg tatggtgatt acaaagttta tgacgtccgt aagatgatcg cgaaaagcga
     6601 acaggagata ggcaaggcta cagccaaata cttcttttat tctaacatta tgaatttctt
     6661 taagacggaa atcactctgg caaacggaga gatacgcaaa cgacctttaa ttgaaaccaa
     6721 tggggagaca ggtgaaatcg tatgggataa gggccgggac ttcgcgacgg tgagaaaagt
     6781 tttgtccatg ccccaagtca acatagtaaa gaaaactgag gtgcagaccg gagggttttc
     6841 aaaggaatcg attcttccaa aaaggaatag tgataagctc atcgctcgta aaaaggactg
     6901 ggacccgaaa aagtacggtg gcttcgatag ccctacagtt gcctattctg tcctagtagt
     6961 ggcaaaagtt gagaagggaa aatccaagaa actgaagtca gtcaaagaat tattggggat
     7021 aacgattatg gagcgctcgt cttttgaaaa gaaccccatc gacttccttg aggcgaaagg
     7081 ttacaaggaa gtaaaaaagg atctcataat taaactacca aagtatagtc tgtttgagtt
     7141 agaaaatggc cgaaaacgga tgttggctag cgccggagag cttcaaaagg ggaacgaact
     7201 cgcactaccg tctaaatacg tgaatttcct gtatttagcg tcccattacg agaagttgaa
     7261 aggttcacct gaagataacg aacagaagca actttttgtt gagcagcaca aacattatct
     7321 cgacgaaatc atagagcaaa tttcggaatt cagtaagaga gtcatcctag ctgatgccaa
     7381 tctggacaaa gtattaagcg catacaacaa gcacagggat aaacccatac gtgagcaggc
     7441 ggaaaatatt atccatttgt ttactcttac caacctcggc gctccagccg cattcaagta
     7501 ttttgacaca acgatagatc gcaaacgata cacttctacc aaggaggtgc tagacgcgac
     7561 actgattcac caatccatca cgggattata tgaaactcgg atagatttgt cacagcttgg
     7621 gggtgacgga tcccccaaga agaagaggaa agtctcgagc gactacaaag accatgacgg
     7681 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagtgaa gcggccgcca
     7741 gctttc
//
