LOCUS       sequence-30504-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, 
            Skarnes WC, Reiter JF
  TITLE     Floxin, a resource for genetically engineering mouse ESCs.
  JOURNAL   Nat Methods. 2010 Jan . 7(1):50-2.
  PUBMED    19966808
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Jan . 7(1):50-2."
COMMENT     Sequence Label: pFloxin-IRES-Ofd1-Myc
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..71)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    89..223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(113..132)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     167..186
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(567..596)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 catgattacg aattcgcgtc gacgaagttc ctatactttc tagagaatag gaacttcgga
       61 ataggaactt ctcgacgcgg tacccccgga tctagacatg ataagataca ttgatgagtt
      121 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      181 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      241 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      301 ctacagatgt gatatggctg attatgatca ttacttatct agccccccct cgaggtcgct
      361 ctagagcgac cccactgggc tgtaggtggt ggatccgcta gcgatctcac attgccaaaa
      421 gacggcaata tggtggaaaa taacatatag acaaacgcac accggcctta ttccaagcgg
      481 cttcggccag taacgttagg ggggggggag ggagaggggc ggatccacta gtaacggccg
      541 ccagtgtgct ggaattcgcc cttttacaag tcctcttcag aaatgagctt ttgctcccac
      601 atgtcatctg gttcttcatg agaaaatcct ggagaacttt catccttgtc actaggcatc
      661 atcatgtcca ctagtgagca ttctttgccc gattttttgg agctcttatc tgcatttgat
      721 tcttcctgtc tctgctggat aattttcatg tatttttcta aagtattttc acaatgagct
      781 gatttgtctt tctggtcttg aacactcttt tctaattctt cttccatctc catcttcagt
      841 g
//
