LOCUS       sequence-305043-        7977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, 
            Nakamura M, Sasaki E, Shiozawa S, Okano H
  TITLE     A versatile toolbox for knock-in gene targeting based on the 
            Multisite Gateway technology.
  JOURNAL   PLoS One. 2019 Aug 27;14(8):e0221164. doi: 
            10.1371/journal.pone.0221164. eCollection 2019.
  PUBMED    31454364
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-08-27"; volume: "14(8):e0221164. doi"; pages: " 
            10.1371/journal.pone.0221164. eCollection 2019"
COMMENT     Sequence Label: pUC-DEST-hSOX2-EGFP-PuroTK
FEATURES             Location/Qualifiers
     source          1..7977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..23
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15..32
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16..32
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             821..877
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(878..880,881..1591)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(878..880,881..1591)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(920..941)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1181..1200)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     repeat_region   1601..1663
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1834..1858
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1865..2364
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1900..1923)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2297..2316
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2388..2984
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2388..2407)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2884..2904
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2991..3986
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    complement(4352..4376)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   4650..4684
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(5312..5332)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5393..5409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5393..5409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5406..5428)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5417..5433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5441..5447,5448..5465,5466..5471))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5486..5507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5624..5641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5795..6383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5875..5894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6554..7345,7346..7414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7177..7196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7415..7519)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7587..7605
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7643..7665)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7765..7784
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcggga
       61 tatctgaatt atcaactttg tataataaag ttgttgccac catgggcaac ttttgtcgga
      121 gacggagaag cggccgttca tcgacgaggc taagcggctg cgagcgctgc acatgaagga
      181 gcacccggat tataaatacc ggccccggcg gaaaaccaag acgctcatga agaaggataa
      241 gtacacgctg cccggcgggc tgctggcccc cggcggcaat agcatggcga gcggggtcgg
      301 ggtgggcgcc ggcctgggcg cgggcgtgaa ccagcgcatg gacagttacg cgcacatgaa
      361 cggctggagc aacggcagct acagcatgat gcaggaccag ctgggctacc cgcagcaccc
      421 gggcctcaat gcgcacggcg cagcgcagat gcagcccatg caccgctacg acgtgagcgc
      481 cctgcagtac aactccatga ccagctcgca gacctacatg aacggctcgc ccacctacag
      541 catgtcctac tcgcagcagg gcacccctgg catggctctt ggctccatgg gttcggtggt
      601 caagtccgag gccagctcca gcccccctgt ggttacctct tcctcccact ccagggcgcc
      661 ctgccaggcc ggggacctcc gggacatgat cagcatgtat ctccccggcg ccgaggtgcc
      721 ggaacccgcc gcccccagca gacttcacat gtcccagcac taccagagcg gcccggtgcc
      781 cggcacggcc attaacggca cactgcccct ctcacacatg gccacgaact tctctctgtt
      841 aaagcaagca ggagacgtgg aagaaaaccc cggtcctgtt agcaagggcg aggagctgtt
      901 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      961 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1021 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1081 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1141 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1201 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1261 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1321 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1381 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1441 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1501 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1561 gatcactctc ggcatggacg agctgtacaa ggaattttaa ccctagaaag ataatcatat
     1621 tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta tcggtctgta
     1681 tatcgaggtc ccgtgaggcg tttgaataga tattaagttt tattatattt acacttacat
     1741 actaataata aattcaacaa acaattcttt tacgtgactt ttaagattta actcaaacaa
     1801 aaactcaaaa tttcttctat aaagtaacaa aactcaagtt tgtacaaaaa agcagaattc
     1861 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     1921 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     1981 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     2041 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     2101 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     2161 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     2221 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     2281 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     2341 tcttcctcat ctccgggcct ttcgaccgat catcaagctt gatcctcatg accgagtaca
     2401 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     2461 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     2521 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     2581 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     2641 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     2701 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     2761 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     2821 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     2881 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     2941 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcc atgcccacgc
     3001 tactgcgggt ttatatagac ggtccccacg ggatggggaa aaccaccacc acgcaactgc
     3061 tggtggccct gggttcgcgc gacgatatcg tctacgtacc cgagccgatg acttactggc
     3121 gggtgctggg ggcttccgag acaatcgcga acatctacac cacacaacac cgcctcgacc
     3181 agggtgagat atcggccggg gacgcggcgg tggtaatgac aagcgcccag ataacaatgg
     3241 gcatgcctta tgccgtgacc gacgccgttc tggctcctca tatcgggggg gaggctggga
     3301 gctcacatgc cccgcccccg gccctcaccc tcatcttcga ccgccatccc atcgccgccc
     3361 tcctgtgcta cccggccgcg cggtacctta tgggcagcat gaccccccag gccgtgctgg
     3421 cgttcgtggc cctcatcccg ccgaccttgc ccggcaccaa catcgtgctt ggggcccttc
     3481 cggaggacag acacatcgac cgcctggcca aacgccagcg ccccggcgag cggctggacc
     3541 tggctatgct ggctgcgatt cgccgcgttt acgggctact tgccaatacg gtgcggtatc
     3601 tgcagtgcgg cgggtcgtgg cgggaggact ggggacagct ttcggggacg gccgtgccgc
     3661 cccagggtgc cgagccccag agcaacgcgg gcccacgacc ccatatcggg gacacgttat
     3721 ttaccctgtt tcgggccccc gagttgctgg cccccaacgg cgacctgtat aacgtgtttg
     3781 cctgggcctt ggacgtcttg gccaaacgcc tccgttccat gcacgtcttt atcctggatt
     3841 acgaccaatc gcccgccggc tgccgggacg ccctgctgca acttacctcc gggatggtcc
     3901 agacccacgt caccaccccc ggctccatac cgacgatatg cgacctggcg cgcacgtttg
     3961 cccgggagat gggggaggct aactgagctc tagcttatcg actcgccgat agtggaaacc
     4021 gacgccccag cactcgtccg agggcaaagg aatagtcgag aaattgatga tctattaaac
     4081 aataaagatg tccacatgga agtttttcct gtcatacttt gttaagaagg gtgagaacag
     4141 agtacctaca ttttgaatgg aaggattgga gctacggggg tgggggtggg gtgggattag
     4201 ataaatgcct gctctttact gaaggctctt tactattgct ttatgataat gtttcatagt
     4261 tggatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca
     4321 tgatctatag atcctctaga gtcgagggct gcagcagctt tcttgtacaa agtgggatat
     4381 ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat
     4441 ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg
     4501 actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc
     4561 acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt
     4621 tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct
     4681 agggttaatg agggttggac agcgaactgg aggggggaga aattttcaaa gaaaaacgag
     4741 ggaaatggga ggggtgcaaa agaggagagt aagaaacagc atggagaaaa cccggtacgc
     4801 tcaaaaagaa aaaggaaaaa aaaaaaaatc ccatcaccca cagcaaatga cagctgcaaa
     4861 agagaacacc aatcccatcc acactcacgc aaaaaccgcg atgccgacaa gaaaactttt
     4921 atgagagaga tcctggactt ctttttgggg gactattttt gtacagagaa aacctgggga
     4981 gggtggggag ggcgggggaa tggaccttgt atagatctgg aggaaagaaa gctacgaaaa
     5041 actttttaaa agttctagtg gtacggtagg agctttgcag gaagtttgca aaagtcttta
     5101 ccaataatat ttagagctag tctccaagcg acgaaaaaaa tgttttaata tttgcaagca
     5161 acttttgtac agtatttatc gagataaaca tggcaatcaa aatgtccatt gtttataagc
     5221 tgagaatttg ccaatatttt tcaaggagag gcttcttgct gaattttgat tctgcagctg
     5281 aaatttagga cagttgcaaa cgtgaaaaga acaacttttc tatacaaagt tgatagcttg
     5341 atatcaatac tctagaggat ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc
     5401 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5461 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5521 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5581 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     5641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     7561 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     7621 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7681 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7741 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7801 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     7861 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     7921 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggtttt
//
