LOCUS       sequence-305054-        1728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BRET biosensor for detection of G-alpha-i-GTP in mammalian 
            expression vector pcDNA3.1(+).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pcDNA3.1-G-alpha-i*-BERKY1
FEATURES             Location/Qualifiers
     source          1..1728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..588
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             613..633
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1195..1214)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1354..1605
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1542..1563
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1696..1725
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggatgta taaaatcaaa agggaaagac agcaagcttg gatcaggcgg tagcggggga
       61 tcaggcggta gcgggatggt cttcacactc gaagatttcg ttggggactg gcgacagaca
      121 gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat
      181 ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag
      241 atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc
      301 gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac
      361 tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg
      421 tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac
      481 ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta
      541 accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgga attcggatct
      601 ggtggttcag gcgaaaacct gtattttcag ggcggaagcg gagaagagga ggaaaagaaa
      661 aaacaacagg aggaggaggc ggagaggctg aggaggatac aagaggaaat ggaaaaagaa
      721 agaaagcggc gagaggaaga tgagcagcga cgccgcaagg aggaggaaga gagaaggatg
      781 aaattggaga tggaggcaaa acgcaaacaa gaagaggaag aacggaagaa acgggaggat
      841 gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg gcgcgcccat ggtttcaaag
      901 ggagaagagt tgttcacagg ggttgtaccg attcttgtag aacttgacgg agatgtcaac
      961 gggcacaagt ttagtgtaag tggagaaggc gagggcgatg cgacctatgg aaaactgacc
     1021 cttaaattta tttgtactac gggtaaattg cccgttccct ggccgacatt ggtaactaca
     1081 ttcggatatg gtttgatgtg ctttgcaagg tatccggatc acatgaagca gcatgatttc
     1141 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1201 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1261 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1321 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1381 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1441 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     1501 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1561 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggatc cggtggcacc
     1621 ggtggatcag gctcttctcg aggttactac catggtattt gggtgggtga agaaggtcga
     1681 ctttctcgag gtggagaaca aaaacttatt tctgaagaag atctgtga
//
