LOCUS       sequence-30507-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF
  TITLE     Ofd1, a human disease gene, regulates the length and distal 
            structure of centrioles.
  JOURNAL   Dev Cell. 2010 Mar 16. 18(3):410-24.
  PUBMED    20230748
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Mar 16. 18(3):410-24."
COMMENT     Sequence Label: pFloxin-IRES-Ofd1-Myc-S75F
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(29..76)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    94..228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(118..137)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     172..191
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(572..601)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tatgacatga ttacgaattc gcgtcgacga agttcctata ctttctagag aataggaact
       61 tcggaatagg aacttctcga cgcggtaccc ccggatctag acatgataag atacattgat
      121 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      181 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      241 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      301 aacctctaca gatgtgatat ggctgattat gatcattact tatctagccc cccctcgagg
      361 tcgctctaga gcgaccccac tgggctgtag gtggtggatc cgctagcgat ctcacattgc
      421 caaaagacgg caatatggtg gaaaataaca tatagacaaa cgcacaccgg ccttattcca
      481 agcggcttcg gccagtaacg ttaggggggg gggagggaga ggggcggatc cactagtaac
      541 ggccgccagt gtgctggaat tcgccctttt acaagtcctc ttcagaaatg agcttttgct
      601 cccacatgtc atctggttct tcatgagaaa atcctggaga actttcatcc ttgtcactag
      661 gcatcatcat gtccactagt gagcattctt tgcccgattt tttggagctc ttatctgcat
      721 ttgattcttc ctgtctctgc tggataattt tcatgtattt ttctaaagta ttttc
//
