LOCUS       sequence-305074-         480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutant version of OlyA that senses sphingomyelin without or with 
            cholesterol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Endapally S, Frias D, Grzemska M, Gay A, Tomchick DR, Radhakrishnan 
            A
  TITLE     Molecular Discrimination between Two Conformations of Sphingomyelin 
            in Plasma Membranes.
  JOURNAL   Cell. 2019 Feb 21;176(5):1040-1053.e17. doi: 
            10.1016/j.cell.2018.12.042. Epub 2019 Jan 31.
  PUBMED    30712872
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12.042"; journalName: "Cell"; date: 
            "2019-02-21- 21"; volume: "176"; issue: "5"; pages: "1040-1053.e17"
COMMENT     Sequence Label: pOlyA(E69A)
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             415..432
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             460..477
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggcgtacg cccaatgggt catcattatc atccacaacg tcggatctca agacgtgaag
       61 atcaagaacc tcaaggcttc ctgggggaag cttcacgctg acggtgacaa agacgctgaa
      121 gtctcagcaa gcaactacga aggaaagatc gtcaagcctg acgagaagct ccagatcaac
      181 gcctctggca gatcagatgc cgccgcgggc acgaccggaa ccttcgatct tgtcgaccct
      241 gccgatggcg acaagcaggt ccgccacttc tactgggact ctccctgggg cagcaaaacc
      301 aacacttgga ccgttagcgg ctccaacacc aagtggatga tcgagtactc tgggcagaac
      361 ttggacagcg gtgccctcgg caccatcacc gtcgacacct tgaagaaggg gaacctggtt
      421 ccgcgcggca gcgaaggcaa aagcggctgc ggcctcgagc accaccacca ccaccactga
//
