LOCUS       sequence-305086-       10492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express soluble His-tagged zebrafish Kitlga in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Oltova J, Svoboda O, Machonova O, Svatonova P, Traver D, Kolar M, 
            Bartunek P
  TITLE     Zebrafish Kit ligands cooperate with erythropoietin to promote 
            erythroid cell expansion.
  JOURNAL   Blood Adv. 2020 Dec 8;4(23):5915-5924. doi: 
            10.1182/bloodadvances.2020001700.
  PUBMED    33259600
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2020-12-8-
            8"; volume: "4"; issue: "23"; pages: "5915-5924"
COMMENT     Sequence Label: pAc-­His-­zfKitlga
FEATURES             Location/Qualifiers
     source          1..10492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3170..3294
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3332..3937)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4001..4092
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4017..4034
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4189..4248
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             4294..4311
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5500..6205
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           5588..5960
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(5617..6205)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(6639..6656)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7873..7889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7873..7889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7886..7908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7897..7913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7921..7927,7928..7945,7946..7951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7966..7987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8104..8121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8275..8863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8355..8374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9034..9825,9826..9894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9657..9676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9895..9999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10067..10085
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10123..10145)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10245..10264
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10458..10480
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10472..10489
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10473..10489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatat gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatcttgga
     4261 tcccgggcca tgggaattct acatatggct agccaccatc accatcacca tggtggatcc
     4321 attgaaatag gaaatcccat tacagatgac atcaagaaga tttctttgtt gaaacagaat
     4381 attccaaaag actacaagat tacagtgcgt tatataccta aagaagtgag tggcatgtgc
     4441 tgggtgaagc taaatgtatt tcacttggag gtgagcttaa aaggcctggc gcagaagttt
     4501 gggaacatat cctccaataa agataatata ggcactttcg tccaaatact gcaagatatg
     4561 cggtatcaca ttggacctgg tttggaggat tcaatgcttg actttgagtg tcactatgta
     4621 gaagagatgt ggcttacggc gaagtatttt gaatttttgg aggatttttt caacactgcc
     4681 aattcatcaa gagatgctga ggactgcgag ccaccgccat gtcccacatc cacaaaaaca
     4741 acaataacaa caactacaac agcgtcgaca acatcagcgc aacatagtac aaatgagtaa
     4801 ggatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg
     4861 aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc
     4921 atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt
     4981 cggaatggac gatgaattcc ggagcggccg ctgcagatct gatcctttcc tgggacccgg
     5041 caagaaccaa aaactcactc tcttcaagga aatccgtaat gttaaacccg acacgatgaa
     5101 gcttgtcgtt ggatggaaag gaaaagagtt ctacagggaa acttggaccc gcttcatgga
     5161 agacagcttc cccattgtta acgaccaaga agtgatggat gttttccttg ttgtcaacat
     5221 gcgtcccact agacccaacc gttgttacaa attcctggcc caacacgctc tgcgttgcga
     5281 ccccgactat gtacctcatg acgtgattag gatcgtcgag ccttcatggg tgggcagcaa
     5341 caacgagtac cgcatcagcc tggctaagaa gggcggcggc tgcccaataa tgaaccttca
     5401 ctctgagtac accaactcgt tcgaacagtt catcgatcgt gtcatctggg agaacttcta
     5461 caagcccatc gtttacatcg gtaccgactc tgctgaagag gaggaaattc tccttgaagt
     5521 ttccctggtg ttcaaagtaa aggagtttgc accagacgca cctctgttca ctggtccggc
     5581 gtattaaaac acgatacatt gttattagta catttattaa gcgctagatt ctgtgcgttg
     5641 ttgatttaca gacaattgtt gtacgtattt taataattca ttaaatttat aatctttagg
     5701 gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct ttatatctga atttaaatat
     5761 taaatcctca atagatttgt aaaataggtt tcgattagtt tcaaacaagg gttgtttttc
     5821 cgaaccgatg gctggactat ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc
     5881 ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc
     5941 gacgtcgttc aaaatattat gcgcttttgt atttctttca tcactgtcgt tagtgtacaa
     6001 ttgactcgac gtaaacacgt taaataaagc ttggacatat ttaacatcgg gcgtgttagc
     6061 tttattaggc cgattatcgt cgtcgtccca accctcgtcg ttagaagttg cttccgaaga
     6121 cgattttgcc atagccacac gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa
     6181 atttgtagtt gagctttttg gaattatttc tgattgcggg cgtttttggg cgggtttcaa
     6241 tctaactgtg cccgatttta attcagacaa cacgttagaa agcgatggtg caggcggtgg
     6301 taacatttca gacggcaaat ctactaatgg cggcggtggt ggagctgatg ataaatctac
     6361 catcggtgga ggcgcaggcg gggctggcgg cggaggcgga ggcggaggtg gtggcggtga
     6421 tgcagacggc ggtttaggct caaatgtctc tttaggcaac acagtcggca cctcaactat
     6481 tgtactggtt tcgggcgccg tttttggttt gaccggtctg agacgagtgc gatttttttc
     6541 gtttctaata gcttccaaca attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc
     6601 cgtcggcatt ggtggagcgg gcggcaattc agacatcgat ggtggtggtg gtggtggagg
     6661 cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt gccgccggta taatttgttc
     6721 tggtttagtt tgttcgcgca cgattgtggg caccggcgca ggcgccgctg gctgcacaac
     6781 ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc aattcaatat tataattgga
     6841 atacaaatcg taaaaatctg ctataagcat tgtaatttcg ctatcgttta ccgtgccgat
     6901 atttaacaac cgctcaatgt aagcaattgt attgtaaaga gattgtctca agctcgcacg
     6961 ccgataacaa gccttttcat ttttactaca gcattgtagt ggcgagacac ttcgctgtcg
     7021 tcgacgtaca tgtatgcttt gttgtcaaaa acgtcgttgg caagctttaa aatatttaaa
     7081 agaacatctc tgttcagcac cactgtgttg tcgtaaatgt tgtttttgat aatttgcgct
     7141 tccgcagtat cgacacgttc aaaaaattga tgcgcatcaa ttttgttgtt cctattattg
     7201 aataaataag attgtacaga ttcatatcta cgattcgtca tggccaccac aaatgctacg
     7261 ctgcaaacgc tggtacaatt ttacgaaaac tgcaaaaacg tcaaaactcg gtataaaata
     7321 atcaacgggc gctttggcaa aatatctatt ttatcgcaca agcccactag caaattgtat
     7381 ttgcagaaaa caatttcggc gcacaatttt aacgctgacg aaataaaagt tcaccagtta
     7441 atgagcgacc acccaaattt tataaaaatc tattttaatc acggttccat caacaaccaa
     7501 gtgatcgtga tggactacat tgactgtccc gatttatttg aaacactaca aattaaaggc
     7561 gagctttcgt accaacttgt tagcaatatt attagacagc tgtgtgaagc gctcaacgat
     7621 ttgcacaagc acaatttcat acacaacgac ataaaactcg aaaatgtctt atatttcgaa
     7681 gcacttgatc gcgtgtatgt ttgcgattac ggattgtgca aacacgaaaa ctcacttagc
     7741 gtgcacgacg gcacgttgga gtattttagt ccggaaaaaa ttcgacacca caactatgca
     7801 cgttcgtttg actggtacgc cgtcggcgtg ttaacataca agttgctaac cggcggccaa
     7861 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7921 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7981 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8041 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8101 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8161 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8221 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8281 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8341 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8401 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8461 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8521 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8581 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8641 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8701 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8881 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10021 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    10081 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    10141 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    10201 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    10261 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    10321 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    10381 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    10441 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg cc
//
