LOCUS       sequence-305087-        9375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of GFP with a nuclear localization sequence, under 
            the human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Miller JD, Ganat YM, Kishinevsky S, Bowman RL, Liu B, Tu EY, Mandal 
            PK, Vera E, Shim JW, Kriks S, Taldone T, Fusaki N, Tomishima MJ, 
            Krainc D, Milner TA, Rossi DJ, Studer L
  TITLE     Human iPSC-based modeling of late-onset disease via progerin-induced
            aging.
  JOURNAL   Cell Stem Cell. 2013 Dec 5;13(6):691-705. doi: 
            10.1016/j.stem.2013.11.006.
  PUBMED    24315443
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11"; journalName: "Cell Stem Cell"; date: 
            "2013-12-5- 5"; volume: "13"; issue: "6"; pages: "691-705"
COMMENT     Sequence Label: pLenti-hSyn-nucGFP
FEATURES             Location/Qualifiers
     source          1..9375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    207..228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(243..248,249..266,267..273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    281..297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     286..308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     305..321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     305..321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    370..491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(381..400)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     435..454
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(621..995)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1014..1061)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1109..1438)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1123..1258
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1177..1196)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1423..1443
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1452..1880)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1562..1583)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1774..1793
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1926..2150
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2139..2156
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2182..2362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     2867..2883
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2868..2884
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2887..3475
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2993..3004
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3402..3422
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3521..3541)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3545..3565)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3569..3589)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3614..4330)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        complement(4344..4791)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    4866..4983
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5281..5325)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5510..5743
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6236..6361
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6408..6588
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6606..6804)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6631..6651)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6806..7185
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7357..7376
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7451..7555
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7556..7624,7625..8416)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7774..7793)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8587..9175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9076..9095
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
       61 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      121 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      181 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      241 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      301 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgacggtata
      361 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      421 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      481 ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
      541 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
      601 tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac gcagttgccg
      661 gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc ggtcatggcc
      721 ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc gtacagctcg
      781 tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg gtcctggacc
      841 gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag
      901 tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg
      961 gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac cttgtcgtat
     1021 tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga tcagatccga
     1081 aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca aaaaagcctc
     1141 ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca taaataaaaa
     1201 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     1261 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     1321 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     1381 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     1441 tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     1501 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     1561 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     1621 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     1681 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     1741 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     1801 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     1861 tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc
     1921 agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
     1981 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
     2041 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
     2101 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga
     2161 tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     2221 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     2281 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     2341 tctggtctaa ccagagagac ccagtacagt ccggatgcag ctctcgggcc atgtgatgaa
     2401 atgctaggcg gctgtcaaac ctccactcta atacttctct ctccgggtca tccatcccat
     2461 gcaggctcac agggtgtaac aagcgggtgt tctctccttc attggcttct tctaccttct
     2521 cttgctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat ctgatccctg
     2581 gccctggtgt gtagttctgc caatcaggga agtagccttg tgtgtggtag atccacagat
     2641 caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta
     2701 aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt acctgaggtg
     2761 tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa tcagcattgg
     2821 tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga ggtcgacggt
     2881 atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     2941 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     3001 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
     3061 gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
     3121 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     3181 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     3241 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     3301 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     3361 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     3421 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattatcga
     3481 taagcttgat atcgaattct tatctagatc cggtggatcc tacctttctc ttcttttttg
     3541 gatctacctt tctcttcttt tttggatcta cctttctctt cttttttgga tcagctcgag
     3601 atctgagtcc ggacttgtac agctcatcca tgccgtgggt gatggcggcg gcggtcacga
     3661 agccgaagta gatcatgtga tcgcgcttct cgttggggtc cttggacagg gcgctctggg
     3721 tggacaggta gtggttatcg ggcagcagca cagggccatc gccgatgggg gtattctgct
     3781 ggtagtggtc ggccagctgc acgctgccat cctcgatgtt gtggcggatc ttgaagttca
     3841 ccttgatgcc attcttggcc ttgtcggtca tgatgtacac attgtgggcg ttgtagttgt
     3901 actccatctt attgcccagg atgttgccat cctccttgaa atcggtgccg gtcagctcga
     3961 tgcgattcac cagggtatcg ccctcgaact tcacctcggc gcgcgacttg tagttgccgt
     4021 catcctcgaa gaagatggtg cgctcctgga tgtagccctc aggcatggcg ctcttgaaga
     4081 agtcgtgctg cttcatgtga tcggggtagc gtgagaagca ctgcacgccg tagctcaggg
     4141 tggtcaccag ggtgggccag ggcacaggca gcttgccggt ggtgcagatg aacttcaggg
     4201 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgct cacgctgaac ttgtggccat
     4261 tcacatcgcc attcagctcg atcaggatgg gcacgatgcc ggtgaacagc tcggcgccct
     4321 tgctcaccat accggtttct cgactgcgct ctcaggcacg acacgactcc tccgctgccc
     4381 accgcagact gaggcagcgc tgagtcgccg gcgccgcagc gcagatggtc gcgcccgtgc
     4441 ccccctatct cgcgcctcgc gtggtgcggt ccggctgggc cggcggcggc gcggacgcga
     4501 ccaaggtggc cgggaagggg agtttgcggg ggaccggcga gtgacgtcag cgcgccttca
     4561 gtgctgaggc ggcggtggcg cgcgccgcca ggcgggggcg aaggcactgt ccgcggtgct
     4621 gaagctggca gtgcgcacgc gcctcgccgc atcctgtttc ccctccccct ctctgatagg
     4681 ggatgcgcaa tttggggaat gggggttggg tgcttgtcca gtgggtcggg gtcggtcgtc
     4741 aggtaggcac ccccaccccg cctcatcctg gtcctaaaac ccacttgcac tcatacgcag
     4801 ggccctctgc agtctagaca cttaattaac caaactggat ctctgctgtc cctgtaataa
     4861 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     4921 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     4981 aaattgtgga tgaatactgc catttgtctg cagaattggc gcacgcagtg ccgatccgtt
     5041 cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg
     5101 cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
     5161 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
     5221 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
     5281 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     5341 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     5401 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     5461 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     5521 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     5581 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     5641 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     5701 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     5761 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     5821 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg
     5881 tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
     5941 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
     6001 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
     6061 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
     6121 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
     6181 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
     6241 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
     6301 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
     6361 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
     6421 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     6481 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     6541 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa
     6601 acgcgctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     6661 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     6721 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     6781 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     6841 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     6901 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     6961 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     7021 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     7081 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     7141 accccgtaat tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg
     7201 cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt
     7261 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca
     7321 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga
     7381 gagtgcacca taggggatcg ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg
     7441 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7501 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7561 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7621 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7681 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7741 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7801 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7861 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7921 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7981 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8041 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8101 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8161 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8221 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8281 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8341 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8401 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8461 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8521 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8701 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8941 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9001 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9241 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9301 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9361 cccaatacgc aaacc
//
