LOCUS       sequence-30509-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF
  TITLE     Ofd1, a human disease gene, regulates the length and distal 
            structure of centrioles.
  JOURNAL   Dev Cell. 2010 Mar 16. 18(3):410-24.
  PUBMED    20230748
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Mar 16. 18(3):410-24."
COMMENT     Sequence Label: pFloxin-IRES-Ofd1-Myc-A80T
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(32..79)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    97..231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(121..140)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     175..194
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(575..604)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agctatgaca tgattacgaa ttcgcgtcga cgaagttcct atactttcta gagaatagga
       61 acttcggaat aggaacttct cgacgcggta cccccggatc tagacatgat aagatacatt
      121 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
      181 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
      241 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
      301 taaaacctct acagatgtga tatggctgat tatgatcatt acttatctag ccccccctcg
      361 aggtcgctct agagcgaccc cactgggctg taggtggtgg atccgctagc gatctcacat
      421 tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt
      481 ccaagcggct tcggccagta acgttagggg ggggggaggg agaggggcgg atccactagt
      541 aacggccgcc agtgtgctgg aattcgccct tttacaagtc ctcttcagaa atgagctttt
      601 gctcccacat gtcatctggt tcttcatgag aaaatcctgg agaactttca tccttgtcac
      661 taggcatcat catgtccact agtgagcatt ctttgcccga ttttttggag ctcttatctg
      721 catttgattc ttcctgtctc tgctggataa ttttcatgta tttttctaaa gtattttcac
      781 aatgagctga t
//
