LOCUS       sequence-305090-       11122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of Progerin with a GFP fusion protein, under human 
            synapsin promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Miller JD, Ganat YM, Kishinevsky S, Bowman RL, Liu B, Tu EY, Mandal 
            PK, Vera E, Shim JW, Kriks S, Taldone T, Fusaki N, Tomishima MJ, 
            Krainc D, Milner TA, Rossi DJ, Studer L
  TITLE     Human iPSC-based modeling of late-onset disease via progerin-induced
            aging.
  JOURNAL   Cell Stem Cell. 2013 Dec 5;13(6):691-705. doi: 
            10.1016/j.stem.2013.11.006.
  PUBMED    24315443
REFERENCE   2  (bases 1 to 11122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11"; journalName: "Cell Stem Cell"; date: 
            "2013-12-5- 5"; volume: "13"; issue: "6"; pages: "691-705"
COMMENT     Sequence Label: pLenti-hSyn-PROGERIN
FEATURES             Location/Qualifiers
     source          1..11122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        77..181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(182..250,251..1042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(400..419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1213..1801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1702..1721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1955..1972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2089..2110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2125..2130,2131..2148,2149..2155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2163..2179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2168..2190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2187..2203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2187..2203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2252..2373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2263..2282)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2317..2336
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2503..2877)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2896..2943)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2991..3320)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3005..3140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3059..3078)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3305..3325
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3334..3762)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3444..3465)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3656..3675
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3808..4032
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4021..4038
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4064..4244
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4749..4765
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4750..4766
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4769..5357
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4875..4886
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5284..5304
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7332..8048)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        complement(8062..8509)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    8584..8701
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8999..9043)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9228..9461
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9954..10079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10126..10306
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10324..10522)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        10524..10903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     11075..11094
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
       61 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      301 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      421 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      721 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2041 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2101 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2161 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     2221 ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc
     2281 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2341 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
     2401 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     2461 ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc
     2521 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
     2581 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
     2641 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc
     2701 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
     2761 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
     2821 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt
     2881 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
     2941 aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag
     3001 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc
     3061 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     3121 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     3181 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     3241 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     3301 ccacacccta actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca
     3361 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     3421 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     3481 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     3541 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3601 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3661 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3721 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
     3781 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     3841 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     3901 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     3961 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     4021 tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctgctaga gattttccac
     4081 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     4141 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     4201 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca gtccggatgc
     4261 agctctcggg ccatgtgatg aaatgctagg cggctgtcaa acctccactc taatacttct
     4321 ctctccgggt catccatccc atgcaggctc acagggtgta acaagcgggt gttctctcct
     4381 tcattggctt cttctacctt ctcttgctca actggtacta gcttgtagca ccatccaaag
     4441 gtcagtggat atctgatccc tggccctggt gtgtagttct gccaatcagg gaagtagcct
     4501 tgtgtgtggt agatccacag atcaaggata tcttgtcttc gttgggagtg aattagccct
     4561 tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt
     4621 ggtcttaaag gtacctgagg tgtgactgga aaacccacct cctcctcctc ttgtgcttct
     4681 agccaggcac aatcagcatt ggtagctgct gtattgctac ttgtgattgc tccatgtttt
     4741 tctaggtctc gaggtcgacg gtatcgatgc ggggaggcgg cccaaaggga gatccgactc
     4801 gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa
     4861 ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc
     4921 caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg
     4981 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
     5041 agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg
     5101 gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt
     5161 tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag
     5221 gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat
     5281 ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg
     5341 taatccagag gttgattatc gataagcttg atatcgaatt cttacatgat gctgcagttc
     5401 tgggggctct gggctcctga gccgctggca gatgccttgt cggcaggctg cccgcaggtc
     5461 ccgcacagca cggtgcgcga gcgcaggttg tactcagcgg ggtcccccga gctgctgcag
     5521 tgggagccat ggtggtgatg gagcaggtca tctccatcct catcctcgtc gtcctcaacc
     5581 acagtcactg agcgcaccag cttgcgcatg gccacttctt ccccagtgga gttgatgaga
     5641 gccgtacgca ggctgttccc gcagccccag gtgttctgtg ccttccacac caggtcggta
     5701 ggggggctgt gggtggcccc agctcctgca gcccagatcg tcaccacctg cccagccttc
     5761 agggtgaact ttggtgggaa ccggtaagtc agcaagggat catctccatt ctggcgcttg
     5821 atctgccaat tgcccatgga ctggtcctca ttggacttgt tgcgcagccg gacaaacttg
     5881 ccctcctcat ccacctcctc cacggccacg cgcccgctag tgcgtgcgtg ctgtgagaag
     5941 ctgctgcggc tctcagtgga ctccagtttg cgctttttgg tgacgctgcc cccaccctgt
     6001 gtctgggatg agtgagagga agcacggcca cggctgcgct gcgaggtagg gctgggggac
     6061 aggcgtagcc tctcctcctc gccctccaag agcttgcggt aggcgtggat ctccatgtcc
     6121 agggccagct tgatgtccag aagctcctgg tactcgtcca gctgctgctg catccttgcc
     6181 cgcatctcgg ccatctcccg ctccttttcc gccagcagcc gccggctggt gtcccgctca
     6241 cgggccagtg agtcctccag gtctcgaagc ttcgcctcct tggctgccag ctgcttctgg
     6301 agctggctga gctgggcaga gaggctgtcg atgcggatgc gcgactgctg cagctcctcg
     6361 tgggcagccc ccaccaggtt gctgttcctc tcagcagact gcctggcatt gtccagcttg
     6421 gcagaataag tcttctccag ctccttctta tactgctcca cctggtcctc atgctgggcc
     6481 cgcagttcct gcagcgcatc cgccagccgg ctctcaaact cacgctgctt cccattgtca
     6541 atctccacca gtcgggtctc atgacggcgc ttggtctcac gcagctcctc actgtagatg
     6601 ttcttctgga agtccagttc ctccttcatg gtctgcagcc tgttctcagc atccacccgc
     6661 cgcagcatct catcctgaag ttgcttcttg gcctcaccta gggctgcctc aagcttggcc
     6721 acctggcccc gcagatcatg cagctcgccc tccagcgtgc gcttctcact gagagcagtg
     6781 ctcagtgcgg cctccttgga gttcagcaga gcctccaggt ccttcagccg agcctgagca
     6841 gctatcaggt caccctcctt cttggtattg cgcgctttca gctccttaaa ctcctcacgc
     6901 actttgctca gctccagctg caggcgggcg cgctccttgg ctactgagtc aagggtcttg
     6961 cgggcatccc cgagctcggc ctcgtaggcg gccttgatgc cggacacctc gcggctgacc
     7021 acctcttcag actcggtgat gcgaaggcgc agccctgcgt tctccgtttc cagcgagcgc
     7081 acacggtcga tgtagaccgc caagcgatca ttgagctcct gcaggtcctc cttctcctgc
     7141 agccgggtga tgcgggtggg cgacagcgga gtggagctgg cctgcgcccc gctgcgggtg
     7201 gcgcgccgct gggacggggt ctccatggcc ggcaggttgg cagcgctgcc cgcggggcag
     7261 gggtcccgga aagggcgcgg ggctcgggtc gaaggacaga gactgctgtc gacagaggcc
     7321 ttaagtccgg acttgtacag ctcatccatg ccgtgggtga tggcggcggc ggtcacgaag
     7381 ccgaagtaga tcatgtgatc gcgcttctcg ttggggtcct tggacagggc gctctgggtg
     7441 gacaggtagt ggttatcggg cagcagcaca gggccatcgc cgatgggggt attctgctgg
     7501 tagtggtcgg ccagctgcac gctgccatcc tcgatgttgt ggcggatctt gaagttcacc
     7561 ttgatgccat tcttggcctt gtcggtcatg atgtacacat tgtgggcgtt gtagttgtac
     7621 tccatcttat tgcccaggat gttgccatcc tccttgaaat cggtgccggt cagctcgatg
     7681 cgattcacca gggtatcgcc ctcgaacttc acctcggcgc gcgacttgta gttgccgtca
     7741 tcctcgaaga agatggtgcg ctcctggatg tagccctcag gcatggcgct cttgaagaag
     7801 tcgtgctgct tcatgtgatc ggggtagcgt gagaagcact gcacgccgta gctcagggtg
     7861 gtcaccaggg tgggccaggg cacaggcagc ttgccggtgg tgcagatgaa cttcagggtc
     7921 agcttgccgt aggtggcatc gccctcgccc tcgccgctca cgctgaactt gtggccattc
     7981 acatcgccat tcagctcgat caggatgggc acgatgccgg tgaacagctc ggcgcccttg
     8041 ctcaccatac cggtttctcg actgcgctct caggcacgac acgactcctc cgctgcccac
     8101 cgcagactga ggcagcgctg agtcgccggc gccgcagcgc agatggtcgc gcccgtgccc
     8161 ccctatctcg cgcctcgcgt ggtgcggtcc ggctgggccg gcggcggcgc ggacgcgacc
     8221 aaggtggccg ggaaggggag tttgcggggg accggcgagt gacgtcagcg cgccttcagt
     8281 gctgaggcgg cggtggcgcg cgccgccagg cgggggcgaa ggcactgtcc gcggtgctga
     8341 agctggcagt gcgcacgcgc ctcgccgcat cctgtttccc ctccccctct ctgatagggg
     8401 atgcgcaatt tggggaatgg gggttgggtg cttgtccagt gggtcggggt cggtcgtcag
     8461 gtaggcaccc ccaccccgcc tcatcctggt cctaaaaccc acttgcactc atacgcaggg
     8521 ccctctgcag tctagacact taattaacca aactggatct ctgctgtccc tgtaataaac
     8581 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     8641 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     8701 attgtggatg aatactgcca tttgtctgca gaattggcgc acgcagtgcc gatccgttca
     8761 ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc
     8821 tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc
     8881 taactctatt cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta
     8941 ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca
     9001 aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg
     9061 attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca
     9121 tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag
     9181 gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat
     9241 cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg
     9301 tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca
     9361 ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag
     9421 tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt
     9481 ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta
     9541 ctactttata tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc
     9601 tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc
     9661 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt
     9721 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc
     9781 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat
     9841 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
     9901 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
     9961 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
    10021 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc
    10081 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc
    10141 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
    10201 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
    10261 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaac
    10321 gcgctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
    10381 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
    10441 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
    10501 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
    10561 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
    10621 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
    10681 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
    10741 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
    10801 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
    10861 cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc
    10921 cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg
    10981 gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac
    11041 acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga
    11101 gtgcaccata ggggatcggg ag
//
