LOCUS       sequence-305110-       11751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BE-P(AP)3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Tan J, Zhang F, Karcher D, Bock R
  TITLE     Engineering of high-precision base editors for site-specific single 
            nucleotide replacement.
  JOURNAL   Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.
  PUBMED    30683865
REFERENCE   2  (bases 1 to 11751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-25"; volume: "10(1):439. doi"; pages: " 
            10.1038/s41467-018-08034-8"
COMMENT     Sequence Label: pJT43_GalL_BE-P(AP)3
FEATURES             Location/Qualifiers
     source          1..11751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1439..1462
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     1483..1499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1483..1499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1513..2199
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(2221..2247,2248..2250,2251..6324)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2221..6324
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6337..6585
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6598..6618
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6634..6881
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6650..6668)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6899..6918)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6900..6918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6928..6945)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6928..6944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6937..6959)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7085..7540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7172..7191)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7382..7403
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7841..8248
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8249..9343
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9837..9856)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9956..9978
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10016..10034)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    10076..10579
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10616..10720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10721..10789,10790..11581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10939..10958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcgt gacagccctc cgaaggaaga ctctcctccg
     1081 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc
     1141 tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca
     1201 gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa
     1261 tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga
     1321 tctattaacg gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca
     1381 ttttcggttt gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa
     1441 tatacctcta tactttaacg tcaaggagaa aaaaccccgg attctagaac tagtggatcc
     1501 cccgggaaaa aaatgagttc cgagacaggc cctgtagctg ttgatcccac tctgaggaga
     1561 agaattgagc cccacgagtt tgaagtcttc tttgaccccc gggaacttcg gaaagagacc
     1621 tgtctgctgt atgagatcaa ctggggagga aggcacagca tctggcgaca cacgagccaa
     1681 aacaccaaca aacacgttga agtcaatttc atagaaaaat ttactacaga aagatacttt
     1741 tgtccaaaca ccagatgctc cattacctgg ttcctgtcct ggagtccctg tggggagtgc
     1801 tccagggcca ttacagaatt tttgagccga tacccccatg taactctgtt tatttatata
     1861 gcacggcttt atcaccacgc agatcctcga aatcggcaag gactcaggga ccttattagc
     1921 agcggtgtta ctatccagat catgacggag caagagtctg gctactgctg gaggaatttt
     1981 gtcaactact ccccttcgaa tgaagctcat tggccaaggt acccccatct gtgggtgagg
     2041 ctgtacgtac tggaactcta ctgcatcatt ttaggacttc caccctgttt aaatatttta
     2101 agaagaaaac aacctcaact cacgtttttc acgattgctc ttcaaagctg ccattaccaa
     2161 aggctaccac cccacatcct gtgggccaca gggttgaaac cagctccagc accagcccct
     2221 atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ttgggccgtc
     2281 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc
     2341 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa
     2401 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc
     2461 tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg
     2521 ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc
     2581 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag
     2641 aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat
     2701 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat
     2761 gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg
     2821 atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg
     2881 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat
     2941 cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa
     3001 gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc
     3061 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt
     3121 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt
     3181 atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga
     3241 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc
     3301 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg
     3361 gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc
     3421 aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac
     3481 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt
     3541 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc
     3601 agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa
     3661 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa
     3721 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt
     3781 tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg
     3841 tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc
     3901 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc
     3961 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc
     4021 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc
     4081 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct
     4141 catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg
     4201 cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg
     4261 gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac
     4321 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt
     4381 cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc
     4441 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt
     4501 atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg
     4561 atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca
     4621 gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg
     4681 gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat
     4741 atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc
     4801 gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa
     4861 aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg
     4921 actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag
     4981 cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac
     5041 accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct
     5101 aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat
     5161 taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa
     5221 tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa
     5281 atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc
     5341 aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga
     5401 ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc
     5461 gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta
     5521 cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc
     5581 gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct
     5641 tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc
     5701 aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac
     5761 tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag
     5821 tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg
     5881 cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc
     5941 cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa
     6001 caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg
     6061 atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag
     6121 cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg
     6181 cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag
     6241 gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc
     6301 gacctctctc agctcggtgg agactctggt ggttcaacta atttatctga tattattgaa
     6361 aaagaaacag gtaaacaatt agttattcaa gaatcaattt taatgttgcc agaagaagtt
     6421 gaagaagtta ttggtaataa accagaatct gatattttag ttcatactgc ttatgatgaa
     6481 tctactgatg aaaatgttat gttattgact tcagatgctc cagaatataa accatgggct
     6541 ttagttattc aagattctaa tggtgaaaat aaaattaaaa tgttgtctgg tggttcaccc
     6601 aagaagaaga ggaaggtgtg atctcttctc gagtcatgta attagttatg tcacgcttac
     6661 attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
     6721 tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc
     6781 aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct
     6841 tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccggtac ccaattcgcc
     6901 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     6961 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     7021 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7081 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7141 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7201 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7261 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7321 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7381 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7441 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7501 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     7561 ttacgcatct gtgcggtatt tcacaccgca tatcgaccgg tcgaggagaa cttctagtat
     7621 atctacatac ctaatattat tgccttatta aaaatggaat cccaacaatt acatcaaaat
     7681 ccacattctc ttcaaaatca attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt
     7741 tatatttata ggataattat actctatttc tcaacaagta attggttgtt tggccgagcg
     7801 gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt aactgtggga atactcaggt
     7861 atcgtaagat gcaagagttc gaatctctta gcaaccatta tttttttcct caacataacg
     7921 agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat tttttgttaa
     7981 tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag aaaaaggaaa
     8041 ggtgagagcg ccggaaccgg cttttcatat agaatagaga agcgttcatg actaaatgct
     8101 tgcatcacaa tacttgaagt tgacaatatt atttaaggac ctattgtttt ttccaatagg
     8161 tggttagcaa tcgtcttact ttctaacttt tcttaccttt tacatttcag caatatatat
     8221 atatatattt caaggatata ccattctaat gtctgcccct aagaagatcg tcgttttgcc
     8281 aggtgaccac gttggtcaag aaatcacagc cgaagccatt aaggttctta aagctatttc
     8341 tgatgttcgt tccaatgtca agttcgattt cgaaaatcat ttaattggtg gtgctgctat
     8401 cgatgctaca ggtgttccac ttccagatga ggcgctggaa gcctccaaga aggctgatgc
     8461 cgttttgtta ggtgctgtgg gtggtcctaa atggggtacc ggtagtgtta gacctgaaca
     8521 aggtttacta aaaatccgta aagaacttca attgtacgcc aacttaagac catgtaactt
     8581 tgcatccgac tctcttttag acttatctcc aatcaagcca caatttgcta aaggtactga
     8641 cttcgttgtt gtcagagaat tagtgggagg tatttacttt ggtaagagaa aggaagacga
     8701 tggtgatggt gtcgcttggg atagtgaaca atacaccgtt ccagaagtgc aaagaatcac
     8761 aagaatggcc gctttcatgg ccctacaaca tgagccacca ttgcctattt ggtccttgga
     8821 taaagctaat gttttggcct cttcaagatt atggagaaaa actgtggagg aaaccatcaa
     8881 gaacgaattc cctacattga aggttcaaca tcaattgatt gattctgccg ccatgatcct
     8941 agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt ttggtgatat
     9001 catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt
     9061 ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc acggttctgc
     9121 tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg ctgcaatgat
     9181 gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa
     9241 ggttttggat gcaggtatca gaactggtga tttaggtggt tccaacagta ccaccgaagt
     9301 cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt ctcttttttt
     9361 atgatatttg tacataaact ttataaatga aattcataat agaaacgaca cgaaattaca
     9421 aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata catttatcaa
     9481 gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact aatggctcaa
     9541 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
     9601 caaaaagtta ggtgtaacag aaaatcatga aactatgatt cctaatttat atattggagg
     9661 attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg
     9721 atggagttta agtcaatacc ttcttgaacc atttcccata atggtgaaag ttccctcaag
     9781 aattttactc tgtcagaaac ggccttaacg acgtagtcga tatggtgcac tctcagtaca
     9841 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     9901 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     9961 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
    10021 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta ggacggatcg
    10081 cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac
    10141 tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta
    10201 gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag
    10261 cgtactttac atatatattt attagacaag aaaagcagat taaatagata tacattcgat
    10321 taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag
    10381 atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg
    10441 ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat
    10501 ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt aaattataat
    10561 tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg
    10621 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10681 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    10741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    10801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    10861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    10921 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    10981 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    11041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    11101 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11161 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    11221 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    11281 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11341 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11401 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    11461 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    11521 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    11581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    11641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    11701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
