LOCUS       sequence-30513-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF
  TITLE     Ofd1, a human disease gene, regulates the length and distal 
            structure of centrioles.
  JOURNAL   Dev Cell. 2010 Mar 16. 18(3):410-24.
  PUBMED    20230748
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Mar 16. 18(3):410-24."
COMMENT     Sequence Label: pFloxin-Ofd1-Myc-S437R
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(48..95)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    113..247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(137..156)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     191..210
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(591..620)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atttcacaca ggaaacagct atgacatgat tacgaattcg cgtcgacgaa gttcctatac
       61 tttctagaga ataggaactt cggaatagga acttctcgac gcggtacccc cggatctaga
      121 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      181 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      241 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      301 ggttttttaa agcaagtaaa acctctacag atgtgatatg gctgattatg atcattactt
      361 atctagcccc ccctcgaggt cgctctagag cgaccccact gggctgtagg tggtggatcc
      421 gctagcgatc tcacattgcc aaaagacggc aatatggtgg aaaataacat atagacaaac
      481 gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg ggagggagag
      541 gggcggatcc actagtaacg gccgccagtg tgctggaatt cgccctttta caagtcctct
      601 tcagaaatga gcttttgctc ccacatgtca tctggttctt catgagaaaa tcctggagaa
      661 ctttcatcct tgtcactagg catcatcatg tccactagtg agcattcttt gcccgatttt
      721 ttggagctct tatctgcatt tgattcttcc tgtctctgct ggataatttt catgtatttt
      781 t
//
