LOCUS       sequence-305135-        6141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Flag tagged human FOXP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Moparthi L, Koch S
  TITLE     A uniform expression library for the exploration of FOX 
            transcription factor biology.
  JOURNAL   Differentiation. 2020 Sep - Oct;115:30-36. doi: 
            10.1016/j.diff.2020.08.002. Epub 2020 Aug 20.
  PUBMED    32858261
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Differentiation. 2020 Sep - Oct"; volume: "115"; pages: "30-36. 
            doi: 10.1016/j.diff.2020.08.002. Epub 2020 Aug 20"
COMMENT     Sequence Label: Flag-FOXP1
FEATURES             Location/Qualifiers
     source          1..6141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             916..924
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    2193..2327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2217..2236)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2271..2290
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2440..2460)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2440..2458)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2479..2495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2479..2495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2492..2514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2503..2519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2527..2533,2534..2551,2552..2557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2572..2593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2710..2727)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2881..3469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2961..2980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3640..4431,4432..4500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4263..4282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4501..4605)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4631..5086)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4768..4789)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4980..4999
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5157..6141
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcagccacc atggattaca aggacgacga cgataaggga
      121 tcccaagaat ctgggactga gacaaaaagt aacggttcag ccatccagaa tgggtcgggc
      181 ggcagcaacc acttactaga gtgcggcggt cttcgggagg ggcggtccaa cggagagacg
      241 ccggccgtgg acatcggggc agctgacctc gcccacgccc agcagcagca gcaacaggca
      301 cttcaggtgg caagacagct ccttcttcag cagcaacagc agcagcaagt tagtggatta
      361 aaatctccca agaggaatga caaacaacca gctcttcagg ttcccgtgtc agtggctatg
      421 atgacacctc aagttatcac tccccagcaa atgcagcaga tcctccagca acaagtgctg
      481 agccctcagc agctccaggt tctcctccag cagcagcagg ccctcatgct tcaacagcag
      541 cagcttcaag agttttataa aaaacaacag gaacagttgc agcttcaact tttacaacaa
      601 caacatgctg gaaaacagcc taaagagcaa cagcaggtgg ctacccagca gttggctttt
      661 cagcagcagc ttttacagat gcagcagtta cagcagcagc acctcctgtc tttgcagcgc
      721 caaggccttc tgacaattca gcccgggcag cctgcccttc cccttcaacc tcttgctcaa
      781 ggcatgattc caacagaact gcagcagctc tggaaagaag tgacaagtgc tcatactgca
      841 gaagaaacca caggcaacaa tcacagcagt ttggatctga ccacgacatg tgtctcctcc
      901 tctgcacctt ccaagacctc cttaataatg aacccacatg cctctaccaa tggacagctc
      961 tcagtccaca ctcccaaaag ggaaagtttg tcccatgagg agcaccccca tagccatcct
     1021 ctctatggac atggtgtatg caagtggcca ggctgtgaag cagtgtgcga agatttccaa
     1081 tcatttctaa aacatctcaa cagtgagcat gcgctggacg atagaagtac agcccaatgt
     1141 agagtacaaa tgcaggttgt acagcagtta gagctacagc ttgcaaaaga caaagaacgc
     1201 ctgcaagcca tgatgaccca cctgcatgtg aagtctacag aacccaaagc cgcccctcag
     1261 cccttgaatc tggtatcaag tgtcactctc tccaagtccg catcggaggc ttctccacag
     1321 agcttacctc atactccaac gaccccaacc gcccccctga ctcccgtcac ccaaggcccc
     1381 tctgtcatca caaccaccag catgcacacg gtgggaccca tccgcaggcg gtactcagac
     1441 aaatacaacg tgcccatttc gtcagcagat attgcgcaga accaagaatt ttataagaac
     1501 gcagaagtta gaccaccatt tacatatgca tctttaatta ggcaggccat tctcgaatct
     1561 ccagaaaagc agctaacact aaatgagatc tataactggt tcacacgaat gtttgcttac
     1621 ttccgacgca acgcggccac gtggaagaat gcagtgcgtc ataatcttag tcttcacaag
     1681 tgttttgtgc gagtagaaaa cgttaaaggg gcagtatgga cagtggatga agtagaattc
     1741 caaaaacgaa ggccacaaaa gatcagtggt aacccttccc ttattaaaaa catgcagagc
     1801 agccacgcct actgcacacc tctcaatgca gctttacagg cttcaatggc tgagaatagt
     1861 atacctctat acactaccgc ttccatggga aatcccactc tgggcaactt agccagcgca
     1921 atacgggaag agctgaacgg ggcaatggag cataccaaca gcaacgagag tgacagcagt
     1981 ccaggcagat ctcctatgca agccgtgcat cctgtacacg tcaaagaaga gcccctcgat
     2041 ccagaggaag ctgaagggcc cctgtcctta gtgacaacag ccaaccacag tccagatttt
     2101 gaccatgaca gagattacga agatgaacca gtaaacgagg acatggagtg actatctcga
     2161 gcctctagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
     2221 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2281 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2341 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccg
     2401 cggcgccaat gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
     2461 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2521 tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2641 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2701 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2761 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2821 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2881 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2941 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3001 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3061 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3301 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4621 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     4681 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     4741 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     4801 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     4861 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     4921 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     4981 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     5041 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     5101 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagtcg
     5161 accgccaatt caatatggcg tatatggact catgccaatt caatatggtg gatctggacc
     5221 tgtgccaatt caatatggcg tatatggact cgtgccaatt caatatggtg gatctggacc
     5281 ccagccaatt caatatggcg gacttggcac catgccaatt caatatggcg gacctggcac
     5341 tgtgccaact ggggaggggt ctacttggca cggtgccaag tttgaggagg ggtcttggcc
     5401 ctgtgccaag tccgccatat tgaattggca tggtgccaat aatggcggcc atattggcta
     5461 tatgccagga tcaatatata ggcaatatcc aatatggccc tatgccaata tggctattgg
     5521 ccaggttcaa tactatgtat tggccctatg ccatatagta ttccatatat gggttttcct
     5581 attgacgtag atagcccctc ccaatgggcg gtcccatata ccatatatgg ggcttcctaa
     5641 taccgcccat agccactccc ccattgacgt caatggtctc tatatatggt ctttcctatt
     5701 gacgtcatat gggcggtcct attgacgtat atggcgcctc ccccattgac gtcaattacg
     5761 gtaaatggcc cgcctggctc aatgcccatt gacgtcaata ggaccaccca ccattgacgt
     5821 caatgggatg gctcattgcc cattcatatc cgttctcacg ccccctattg acgtcaatga
     5881 cggtaaatgg cccacttggc agtacatcaa tatctattaa tagtaacttg gcaagtacat
     5941 tactattgga agtacgccag ggtacattgg cagtactccc attgacgtca atggcggtaa
     6001 atggcccgcg atggctgcca agtacatccc cattgacgtc aatggggagg ggcaatgacg
     6061 caaatgggcg ttccattgac gtaaatgggc ggtaggcgtg cctaatggga ggtctatata
     6121 agcaatgctc gtttagggaa c
//
