LOCUS       sequence-30515-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF
  TITLE     Ofd1, a human disease gene, regulates the length and distal 
            structure of centrioles.
  JOURNAL   Dev Cell. 2010 Mar 16. 18(3):410-24.
  PUBMED    20230748
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Mar 16. 18(3):410-24."
COMMENT     Sequence Label: pFloxin-Ofd1-Myc-KDD359-361FSY
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5..21
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(44..91)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    109..243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(133..152)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     187..206
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(587..616)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cacacaggaa acagctatga catgattacg aattcgcgtc gacgaagttc ctatactttc
       61 tagagaatag gaacttcgga ataggaactt ctcgacgcgg tacccccgga tctagacatg
      121 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      241 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
      301 ttttaaagca agtaaaacct ctacagatgt gatatggctg attatgatca ttacttatct
      361 agccccccct cgaggtcgct ctagagcgac cccactgggc tgtaggtggt ggatccgcta
      421 gcgatctcac attgccaaaa gacggcaata tggtggaaaa taacatatag acaaacgcac
      481 accggcctta ttccaagcgg cttcggccag taacgttagg ggggggggag ggagaggggc
      541 ggatccacta gtaacggccg ccagtgtgct ggaattcgcc cttttacaag tcctcttcag
      601 aaatgagctt ttgctcccac atgtcatctg gttcttcatg agaaaatcct ggagaacttt
      661 catccttgtc actaggcatc atcatgtcca ctagtgagca ttctttgccc gattttttgg
      721 agctcttatc tgcatttgat tcttcctgtc tctgctggat aattttcatg tatttttcta
      781 aagtattttc
//
